Microneedle-Based Device for Biological Analysis

Lu, Huiting; Zada, Shah; Yang, Lingzhi; Dong, Haifeng

doi:10.3389/fbioe.2022.851134

Cited by 10 publications

(4 citation statements)

References 133 publications

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“…[7] At present, the implementation of microneedle arrays as interface materials for electrochemical sensing has predominately relied on metallic microneedles (e.g., stainless steel), or metalized coatings consisting of thin metal films (predominately gold and platinum), required to obtain the conductive layer for signal transduction. [10] Although incorporating such materials remain the gold standard due to their well-understood properties, such as chemical stability and electrical consistency, they also lack mechanical flexibility, ease of processibility, and often present poor biocompatibility, in conjunction with loss of functionality over time. [11] Intrinsically conducting polymers such as PPy and PEDOT:PSS can prove impressive alternatives for the development of conductive microneedle surfaces due to their excellent electrical properties even under mechanical stress.…”

Section: Conductive Polymeric Microneedle (Mn) Arrays As Biointerface...mentioning

confidence: 99%

Conductive Polymer‐Coated 3D Printed Microneedles: Biocompatible Platforms for Minimally Invasive Biosensing Interfaces

Keirouz

Mustafa

Turner

et al. 2023

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Conductive polymeric microneedle (MN) arrays as biointerface materials show promise for the minimally invasive monitoring of analytes in biodevices and wearables. There is increasing interest in microneedles as electrodes for biosensing, but efforts have been limited to metallic substrates, which lack biological stability and are associated with high manufacturing costs and laborious fabrication methods, which create translational barriers. In this work, additive manufacturing, which provides the user with design flexibility and upscale manufacturing, is employed to fabricate acrylic‐based microneedle devices. These microneedle devices are used as platforms to produce intrinsically‐conductive, polymer‐based surfaces based on polypyrrole (PPy) and poly(3,4‐ethylenedioxythiophene)‐poly(styrenesulfonate) (PEDOT:PSS). These entirely polymer‐based solid microneedle arrays act as dry conductive electrodes while omitting the requirement of a metallic seed layer. Two distinct coating methods of 3D‐printed solid microneedles, in situ polymerization and drop casting, enable conductive functionality. The microneedle arrays penetrate ex vivo porcine skin grafts without compromising conductivity or microneedle morphology and demonstrate coating durability over multiple penetration cycles. The non‐cytotoxic nature of the conductive microneedles is evaluated using human fibroblast cells. The proposed fabrication strategy offers a compelling approach to manufacturing polymer‐based conductive microneedle surfaces that can be further exploited as platforms for biosensing.

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Section: Conductive Polymeric Microneedle (Mn) Arrays As Biointerface...mentioning

confidence: 99%

Conductive Polymer‐Coated 3D Printed Microneedles: Biocompatible Platforms for Minimally Invasive Biosensing Interfaces

Keirouz

Mustafa

Turner

et al. 2023

Small

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“…A noninvasive myoelectrical control scheme mainly consists of sEMG acquisition [29][30][31][32] and sEMG-based control strategy (figures 1(d) and (e)). Amputees' motion intentions are identified to control hand prostheses voluntarily.…”

Section: Myoelectrical Control Of Bionic Handmentioning

confidence: 99%

Biorealistic hand prosthesis with compliance control and noninvasive somatotopic sensory feedback

Lan

Zhang

et al. 2023

Prog. Biomed. Eng.

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Significant advances have been made to improve control and to provide sensory functions for bionic hands. However, great challenges remain limiting wide acceptance of bionic hands due to inadequate bi-directional neural compatibility with human users. Recent research has brought to light the necessity for matching neuromechanical behaviors between the prosthesis and the sensorimotor system of amputees. A novel approach to achieving greater neural compatibility leverages the technology of biorealistic modeling with real-time computation. These studies have demonstrated a promising outlook that this unique approach may transform the performance of hand prostheses. Simultaneously, a non-invasive technique of somatotopic sensory feedback has been developed based on evoked tactile sensation (ETS) for conveying natural, intuitive and digit-specific tactile information to users. This article reports the recent work in these two important aspects of sensorimotor functions in prosthetic research. A background review is presented first on the state-of-the-art of bionic hand and the various techniques to deliver tactile sensory information to users. Then the progress in developing the novel biorealistic hand prosthesis and the technique of non-invasive ETS feedback is highlighted. Finally, challenges to future development of the biorealistic hand prosthesis and implementing the ETS feedback are discussed with respect to shaping a next-generation hand prosthesis.

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“…1 μL) to provide reliable measurements . Tremendous advances have been achieved in both the design and targets of MN (bio)sensors, with glucose sensors at the forefront of the list. , Yet, there are several challenges to be addressed, including new analytes, proper calibration, reliable validation of on-body data, and the managing of ethical permits, which results in only few investigations reaching true in vivo measurements. − …”

mentioning

confidence: 99%

“…4 Tremendous advances have been achieved in both the design and targets of MN (bio)sensors, with glucose sensors at the forefront of the list. 5,6 Yet, there are several challenges to be addressed, including new analytes, proper calibration, reliable validation of on-body data, and the managing of ethical permits, which results in only few investigations reaching true in vivo measurements. 7−9 Amino acids (AAs) are potential targets of MN biosensors, as the clinical interest in their detection has increased in recent years owing to new discoveris.…”

mentioning

confidence: 99%

Intradermal Glycine Detection with a Wearable Microneedle Biosensor: The First In Vivo Assay

et al. 2022

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Glycine (GLY) is gaining importance in medical diagnoses due to its relationship with multiple physiological functions. Today, GLY is exclusively analyzed using instrumentation centralized in clinical labs, and a tangible point-of-care tool that gathers real-time data from the patient for effective and fast evaluations is lacking. Relevant clinical advances are expected as soon as the rapid provision of both punctual and continuous measurements is possible. In that context, this work presents a microneedle (MN)-based biosensor for intradermal GLY detection in interstitial fluid (ISF). The MN tip is externally tailored to detect GLY levels through the hydrogen peroxide formed in its reaction with a quinoprotein-based GLY oxidase enzyme. The analytical performance of the MN biosensor indicates a fast response time (<7 s); acceptable reversibility, reproducibility, and stability; as well as a wide linear range of response (25–600 μM) that covers the physiological levels of GLY in ISF. The MN biosensor conveniently exhibits high selectivity for GLY over other compounds commonly found in ISF, and the response is not influenced by temperature, pH, or skin insertions. Validated intradermal measurements of GLY were obtained at the in vitro (with pieces of rat skin), ex vivo (on-body tests of euthanized rats) and in vivo (on-body tests of anesthetized rats) levels, demonstrating its ability to produce accurate physiological data. The developed GLY MN biosensor is skin-wearable and provides reliable, real-time intradermal GLY measurements in ISF by means of a minimally invasive approach.

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Microneedle-Based Device for Biological Analysis

Cited by 10 publications

References 133 publications

Conductive Polymer‐Coated 3D Printed Microneedles: Biocompatible Platforms for Minimally Invasive Biosensing Interfaces

Conductive Polymer‐Coated 3D Printed Microneedles: Biocompatible Platforms for Minimally Invasive Biosensing Interfaces

Biorealistic hand prosthesis with compliance control and noninvasive somatotopic sensory feedback

Intradermal Glycine Detection with a Wearable Microneedle Biosensor: The First In Vivo Assay

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