Superoxide Production by Digitonin-Stimulated Guinea Pig Granulocytes

Cohen, Harvey J.; Chovaniec, Margaret E.

doi:10.1172/jci109008

Cited by 199 publications

(62 citation statements)

References 19 publications

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“…The local anesthetic lidocaine (14 mM) reduced markedly both cell aggregation and H2O2 production. This may be additional proof for Hoidal et al (8) who used 1-12 mM lidocaine to inhibit the 02-production by human alveolar macrophages and Cohen et al who obtained similar results involving lidocaine (1-10 mM) inhibition of phorbol myristate acetate-stimulated 02-production in guinea pigs and human granu.locytes (4). In some instances, deposits were seen within some intracellular vesicles or vacuoles other than contacted plasma membranes of associated cells.…”

Section: Methodsmentioning

confidence: 66%

Hydrogen peroxide production stimulated by N-formyl-methionyl-leucyl-phenylalanine in guinea pig macrophages and effects of anti-inflammatory drugs and local anesthetics.

Moriguchi

Hirai

1985

Acta Histochem. Cytochem

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Section: Methodsmentioning

confidence: 66%

Hydrogen peroxide production stimulated by N-formyl-methionyl-leucyl-phenylalanine in guinea pig macrophages and effects of anti-inflammatory drugs and local anesthetics.

Moriguchi

Hirai

1985

Acta Histochem. Cytochem

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“…PMN°2 generation was measured as superoxide dismutase inhibitable cytochrome c reduction as described (38). Duplicate reaction mixtures containing 5 X 106/ml PMN and 75 AM ferricytochrome c were incubated for the indicated time intervals at 37°C in the presence or absence of 10 Mg/ml superoxide dismutase.…”

Section: Introductionmentioning

confidence: 99%

Escherichia coli hemolysin is a potent inductor of phosphoinositide hydrolysis and related metabolic responses in human neutrophils.

Grimminger¹,

Sibelius²,

Bhakdi³

et al. 1991

J. Clin. Invest.

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Escherichia coli hemolysin (Hly) is a proteinaceous pore-forming exotoxin that probably represents a significant virulence factor in E. coli infections. We investigated its influence on human polymorphonuclear neutrophils (PMN), previously identified as highly susceptible targets. Hly provoked rapid secretion of elastase and myeloperoxidase, generation of superoxide, and synthesis of platelet-activating factor (PAF) and lyso-PAF. Concomitantly, marked phosphatidylinositol (Ptdlns) hydrolysis with sequential appearance of the inositolphosphates, inositol triphosphate, diphosphate, and monophosphate, respectively, and formation of diacylglycerol, occurred. The metabolic responses displayed distinct bell-shaped dose dependencies, with maximum events noted at low toxin concentrations of 0.1-0.5 hemolytic units per milliliter. Ptdlns hydrolysis and metabolic responses elicited by Hly exceeded those evoked by optimal concentrations of formylmethionyl-leucyl phenylalanine, PAF, leukotriene B4, A23187, or staphylococcal a-toxin. The toxin-induced effects were sensitive toward modulators of PMN stimulus transmission pathways (pertussis toxin, the kinase C inhibitor H7, and phorbol myristate acetate "priming"). We conclude that the marked capacity of low doses of Hly to elicit degranulation, respiratory burst, and lipid mediator generation in human PMN probably envolves signal transduction via PtdIns hydrolysis. (J. Clin. Invest.

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“…Because the production of 02 by neutrophils is a result of two phenomena: the activation of an 0; generating system and the activity of this enzyme system [6], the results of the above experiments could be the results of an effect of TPTCl on the…”

Section: Effects Of Tptcl On Nadph Oxidase Isolated From Pma-stimulatmentioning

confidence: 97%

Triphenyltin chloride inhibits superoxide production by human neutrophils stimulated with a surface active agent

et al. 1983

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Treatment of human neutrophils with triphenyltin chloride (TPTCl)-inhibited superoxide (0%) production stimulated with phorbol myristate acetate (PMA). TPTCl was more potent as inhibitor of 02 production than other phenyltin compounds. The 0; production by the xanthine oxidase-acetaldehyde system was not inhibited by TPTCI. This finding indicates that TPTCl does not itself react with Or. Furthermore, TPTCI did not influence the isolated NADPH oxidase at all, though 05 production of neutrophils stimulated with PMA in the presence of TPTCl was inhibited. These results indicate that TPTCl inhibits the activation process of the 02 generating system. Triphenyltin chloride SuperoxidePhorbol myristate acetate Neutrophil NADPH oxidase

show abstract

Superoxide Production by Digitonin-Stimulated Guinea Pig Granulocytes

Cited by 199 publications

References 19 publications

Hydrogen peroxide production stimulated by N-formyl-methionyl-leucyl-phenylalanine in guinea pig macrophages and effects of anti-inflammatory drugs and local anesthetics.

Hydrogen peroxide production stimulated by N-formyl-methionyl-leucyl-phenylalanine in guinea pig macrophages and effects of anti-inflammatory drugs and local anesthetics.

Escherichia coli hemolysin is a potent inductor of phosphoinositide hydrolysis and related metabolic responses in human neutrophils.

Triphenyltin chloride inhibits superoxide production by human neutrophils stimulated with a surface active agent

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