Superoxide dismutase as a measure of antioxidant status and its application to diabetes

Omoruyi, Felix O.; Sparks, Jean; Stennett, Dewayne; Dilworth, Lowell

doi:10.1016/b978-0-12-815776-3.00041-3

Cited by 6 publications

(4 citation statements)

References 54 publications

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“…According to Afanas'ev (2010), hyperglycemia here increases ROS (superoxide) through NADPH oxidase and mitochondria. The increase in ROS causes a decrease in the expression of the SOD enzyme (Omoruyi et al, 2020). In this study, the pretest SOD level certainly increased.…”

Section: Effect Of Streptozotocin Induction On Sod and Crp Levelssupporting

confidence: 50%

Taro (Colocasia Esculenta (L.) Schott) Extract didn’t Increase Superoxide Dismutase (SOD) but Decrease C-Reactive Protein (CRP) in Male Wistar Rat (Rattus Norvegicus) Diabetes Mellitus Model

Rachelia¹,

Pinatih²,

Dewi³

2022

IJRP

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Backgrounds:In hyperglycemia induced by streptozotocin, free radicals and oxidative stress conditions occur. This also reduced SOD enzymes while increasing CRP. This study aimed to analyze effect of flavonoid-rich taro leaves ethanol extract on SOD levels and serum CRP levels in rats diabetes melitus model induced by streptozotocin. Methods: Experimental study with a pretest-posttest control group design 26 healthy rats were randomly divided into 2 groups. The control group was given 2 ml aquadest and treatment group was given 400mg/kgBW ethanol extract of taro leaves in 2 ml aquadest. Before and after treatment, blood serum was collected for SOD and CRP analysis using the ELISA technique. Results: SOD level in control goup before and after given plasebo were reduce insignificantly (from 5.37±0.69 ng/ml to 5.26±1.50 ng/ml ;p = 0.830). Furthermore, CRP level in control group before and after given plasebo were not signifcantly different (from 3.39±0.24 ng/ml to 3.56 ±0.43 ng/ml ; p = 0.308). SOD level in treatment group before and after given taro extract were increase but not significant (from 5.33 ±1.14 ng/ml to 6.20 ±2.64 ng/mL; p = 0.239). However, CRP level in control group after given plasebo decrease significantly (from 3.56 ± 0.43 ng/mL to 2.93 ± 0.47 nmol/mL ; p = 0.009).Conclusion: ethanol extract of taro leaves did not increase SOD levels but decrease CRP levels in rats model diabetes melitus induced by streptozotocin. Further studies are needed to confirm extract of taro leaves as external antioxidant.

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Section: Effect Of Streptozotocin Induction On Sod and Crp Levelssupporting

confidence: 50%

Taro (Colocasia Esculenta (L.) Schott) Extract didn’t Increase Superoxide Dismutase (SOD) but Decrease C-Reactive Protein (CRP) in Male Wistar Rat (Rattus Norvegicus) Diabetes Mellitus Model

Rachelia¹,

Pinatih²,

Dewi³

2022

IJRP

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“…[16] ChemistrySelect (O 2 *À ). [4,25] Ultimately, the semi-quinone radical gets converted to o-quinone and generates hydrogen peroxide (H 2 O 2 ). [8,26] Thus, the reaction pathway of oxidation of 4-methyl catechol is believed to involve superoxide radical (O 2 *À ) (Figure 2B).…”

Section: Resultsmentioning

confidence: 99%

Nanoparticles Mimicking Oxidase Activity and their Application in Synthesis of Neurodegenerative Therapeutic Drug L‐DOPA

et al. 2023

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Nanozymes are nanomaterials with intrinsic enzyme‐like activity and show high operational stability as compared to native enzymes. They have gathered the attention of researchers due to their unique physico‐chemical properties and diverse applications. In the present study, screening of different nanoparticles (zinc oxide, iron oxide, selenium and titanium oxide) was performed to evaluate their polyphenol oxidase (PPO) activity. PPO is a biotechnologically important enzyme and its application includes wastewater treatment, food processing and synthesis of l‐3,4‐dihydroxyphenylalanine (L‐dopa, a precursor of dopamine neurotransmitter). Out of all nanoparticles used in this study, zinc oxide nanoparticles (ZnO NPs) showed maximum enzyme activity of 75 %. ZnO NPs demonstrated high thermal stability and can be used upto 80°C. The colloidal solution of nanoparticles showed storage stability upto 6 months at room temperature and showed high activity in the pH range of 6–9 as compared to free enzyme. The nanoparticles were reused till 6th cycle retaining more than 80 % oxidase activity. To study its potential as a catalyst, it was used for the synthesis of L‐dopa from L‐tyrosine and a good productivity of 100 mg/L (0.1 g/L) was obtained. Thus, ZnO nanozymes can be used as an alternate to oxidase enzyme for synthesis of this therapeutic drug.

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“…The compound 1,2,3-benzenetriol, among phenolic antioxidants, is one of the most effective in breaking chain reactions of free radicals (Saluja et al, 2016). Additionally, 1,2,3-benzenetriol acts as a pro-oxidant due to its ability to undergo auto-oxidation in aqueous or alkaline environments, forming superoxide radicals, hydrogen peroxide, and hydroxyl radicals (Omoruyi et al, 2020). It was through this mechanism of auto-oxidation of 1,2,3-benzenetriol that a superoxide scavenging assay was developed to analyze certain antioxidants with reliability and low cost (Zhang et al, 2016;Ramasarma et al, 2015;Li, 2012).…”

Section: Discussionmentioning

confidence: 99%

Parkia from Cerrado: phytochemical bioprospection, toxicity and in vitro bioactivities of bark and flower extracts

Fernandes,

Cardoso,

Alves

et al. 2023

Braz. J. Biol.

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Parkia platycephala is the only species of the genus Parkia that is endemic to the brazilian Cerrado and the tree symbol of the state of Tocantins, but there are still few studies regarding its bioprospecting. In this study, we aimed to investigate the phytochemical composition, toxicity and bioactivities of the bark and flower of Parkia platycephala. Hot sequential extractions (Soxhlet) were performed using methanol and hydroethanolic solution (70%), after degreasing the sample (hexane). The presence of flavonoids, tannins, steroids and alkaloids was detected in the preliminary screening. Trilinolein, (Z)-9-octadecenamide, 3-O-methyl-d-glucose were detected by Gas Chromatography coupled to Mass Spectrometry (GC-MS). In the Liquid Chromatography with Diode Array Detector (LC-PDA) analysis, it was detected exclusively ferulic acid (bark) and ellagic acid (flower). The ethanolic extract of the bark (IC50=10.69 ± 0.35 µgmL-1) has an antioxidant potential (DPPH• radical) higher than that of the rutin standard (IC50=15.85 ± 0.08 µgmL-1). All extracts showed excellent anticholinesterase potential (Ellman), with emphasis on the ethanol extract of the flower (IC50 =5.34 ± 0.12 µgmL-1). Regarding toxicity (Artemia salina), the methanolic extract of the bark and the ethanolic extract of the flower presented high and moderate levels, respectively. Such results limit the concentrations of biological activities in this study, however, the antioxidant and anticholinesterase indices fall short of toxicity. The results demonstrated promising antioxidant and anticholinesterase activities of both the bark and the flower of Parkia platycephala.

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Superoxide dismutase as a measure of antioxidant status and its application to diabetes

Cited by 6 publications

References 54 publications

Taro (Colocasia Esculenta (L.) Schott) Extract didn’t Increase Superoxide Dismutase (SOD) but Decrease C-Reactive Protein (CRP) in Male Wistar Rat (Rattus Norvegicus) Diabetes Mellitus Model

Taro (Colocasia Esculenta (L.) Schott) Extract didn’t Increase Superoxide Dismutase (SOD) but Decrease C-Reactive Protein (CRP) in Male Wistar Rat (Rattus Norvegicus) Diabetes Mellitus Model

Nanoparticles Mimicking Oxidase Activity and their Application in Synthesis of Neurodegenerative Therapeutic Drug L‐DOPA

Parkia from Cerrado: phytochemical bioprospection, toxicity and in vitro bioactivities of bark and flower extracts

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