Superoxide dismutase and Glutathione peroxidase in the blood of dogs with Leishmaniasis

Britti, Domenico; Sconza, Sarah; Morittu, Valeria Maria; Santori, Domenico; Boari, Andrea

doi:10.1007/s11259-008-9121-3

Cited by 18 publications

(23 citation statements)

References 15 publications

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“…Our results on SOD are in agreement with the results of the study by Plevnik Kapun et al [26] in which the same assay kit and sample type were used. Surprisingly, our results differ greatly from the results of Britti et al [49], although the same assay kit and sample type were used in both studies. The difference might be due to different biochemical analysers being used, as well as due to differences in pre-analytical considerations and the dogs included in the study.…”

Section: Discussioncontrasting

confidence: 99%

Plasma Total Antioxidant Capacity and Activities of Blood Glutathione Peroxidase and Superoxide Dismutase Determined in Healthy Dogs by Using Commercially Available Kits

et al. 2016

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Data on the values of selected blood antioxidant parameters, i.e. total antioxidant capacity, glutathione peroxidase, and superoxide dismutase in healthy dogs, are lacking. There are no published accepted standard reference methods for their determination. The aim of this study was to determine the values of plasma total antioxidant capacity and the activities of whole blood glutathione peroxidase and erythrocyte superoxide dismutase in 30 healthy client-owned dogs (19 females, 11 males). The effect of age and sex on the measured antioxidant parameters was also investigated. Antioxidant parameters were determined with an automated biochemical analyser, using the commercially available Randox kits. No signifi cant difference in age, weight, and antioxidant parameters was determined between females and males. A signifi cant positive effect of age (p = 0.002, r² = 0.284) on superoxide dismutase activity was confi rmed. There was no effect of sex on any of the antioxidant parameters measured. However, we observed a tendency of the effect of sex (p = 0.063, r² = 0.118), as well as age (p = 0.073, r² = 0.111), on the activity of glutathione peroxidase. Our results are in part comparable with the results of other studies in which the same types of methods and samples were used to determine antioxidant parameters. In conclusion, the sex and age of dogs should be taken into consideration when planning a study on antioxidant status parameters.

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Section: Discussioncontrasting

confidence: 99%

Plasma Total Antioxidant Capacity and Activities of Blood Glutathione Peroxidase and Superoxide Dismutase Determined in Healthy Dogs by Using Commercially Available Kits

et al. 2016

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“…This result agrees with the study of Britti et al [8], which showed a decrease in GPx levels in CanL, although it was not significant. However, these authors found a positive correlation (P < 0.01) between erythrocytes and GPx activity and between hemoglobin and GPx activity in animals with CanL.…”

Section: Discussionsupporting

confidence: 92%

“…Animals with CanL showed lower (P < 0.001) CAT activity compared to control group, and CAT showed positive correlation with the global erythrocyte count, however it was not significant. Other authors observed an increase in CAT activity in dogs with parvovirus infection [28] and babesiosis [8].…”

Section: Discussionmentioning

confidence: 85%

“…Recent studies have suggested the involvement of reactive oxygen species (ROS) on the pathogenesis of various infectious and parasitic diseases in dogs [10,13,18,20,31,34] including CanL [5,7,8,16]. ROS overproduction can induce an imbalance between oxidants and antioxidants at cellular or systemic level, leading to the establishment of oxidative stress.…”

Section: Introductionmentioning

confidence: 99%

“…At CanL it was verified that the intensity of oxidative stress was dependent on the disease stage and it was associated with increased induced production of superoxide and apoptosis [8,11]. Furthermore, symptomatic dogs for CanL had a decrease on total antioxidant status (TAS) that supplied specific effects on the antioxidant defense mechanisms [16].…”

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confidence: 96%

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Catalase and Glutathione Peroxidase in Dogs Naturally Infected by Leishmania infantum

Lopes-Neto

Santos

Lima

et al. 2016

Acta Scientiae. Vet.

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Background: Canine leishmaniasis (CanL) is caused by an obligatory intracellular parasite of Leishmania genus that affects organs and tissues. Several studies evaluate the role of reactive oxygen species (ROS) in the pathogenesis of many diseases. The overproduction of ROS on infectious diseases can induce an imbalance between oxidants and antioxidants at cellular or systemic level. Thus, the aim of this study was to evaluate the activity of antioxidant enzymes in CanL.Materials, Methods & Results: Females (n = 17) and males (n = 10), at different ages and with different weight, were selected for this study. Dogs were divided into two groups according classical clinical signs and sorological test to CanL. Animals were considered infected based on indirect immunofluorescent assay and ELISA titration ≥ 1:40. Group B (n = 15) composed by positive dogs to CanL from Zoonosis Control Center of Fortaleza (Ceará, Brazil) and group A (n = 12) was composed by dogs from private kennel that were serologically negative to L. infantum and had absence of clinical signs to CanL. Blood sample were collected for evaluation of hematological and biochemical parameters and glutathione peroxidase (GPx) and catalase (CAT) enzymatic activity. Data were analyzed by Student’s t-test and Pearson correlation coefficient (P < 0.05). Total proteins (TP, mg/dL) and alkaline phosphatase (ALP, U/L) were increased (P < 0.05) on group B (8.2 ± 1.2; 165.4 ± 46.4) when compared to group A (6.5 ± 1.1; 109.1 ± 38.3), respectively. Hemoglobin (Hb; g/dL) and hematocrit (Hct; %) were decreased (P < 0.05) on Group B (14.7 ± 1.8; 48.2 ± 5.7) when compared to group A (16.5 ± 1.3; 52.1 ± 2.4), respectively. Group B presented CAT (U/g Hb) and GPx (mU/mg Hb) lower (189.4 ± 90.4; 3,609.6 ± 1,569.1) than group A (326.6 ± 104.5; 5,055.6 ±1,569.1), respectively (P < 0.001). Positive correlation was observed between RBC and CAT; however, it was not significant.Discussion: Organisms require a good defense system in order to revert the overproduction of free radicals and consequently the injuries caused by them. This is possible through the production of antioxidant agents, which act on oxidative prevention and on tissue and cellular regeneration, by taking the reduced glutathione (GSH), superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) enzymes in the frontline. Erythrocyte changes promoted by CanL suggest possible correlation between anemia and the appearance of clinical signs, which in many cases is not seen. Erythrocytes contain SOD, CAT and GPx enzymes, thus, changes in these cells will reflect on the activity of these enzymes. In our results only CAT showed positive correlation with erythrocyte count, however it was not significant. GPx activity was lower (P < 0.001) in infected dogs than control group. This result agrees with another study, which showed a decrease in GPx levels in CanL, although it was not significant. However, it was found a positive correlation (P < 0.001) between erythrocytes and GPx activity and between hemoglobin and GPx activity in animals with leishmaniasis. These results suggest that the reduction in detoxification activity can be related to the decrease in erythrocyte count and that the GPx activity depends on the control mechanism of the antioxidant system in CanL. Furthermore, this result could be associated with decrease of blood cell count in animals with CanL, once GPx is an erythrocyte enzyme, which plays an important role in hemoglobin protection against oxidative damage. This study was carried out in naturally infected dogs with L. infantum. In conclusion, CAT and GPx activities are relate to oxidative stress induced by L. infantum infection and can be used as biomarkers on CanL.

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Superoxide Dismutase and Glutathione Peroxidase in Dogs with Leishmaniasis Following Antimoniate Therapy

Britti

Sconza

Massimini³

et al. 2011

Veterinary Science

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Superoxide dismutase and Glutathione peroxidase in the blood of dogs with Leishmaniasis

Cited by 18 publications

References 15 publications

Plasma Total Antioxidant Capacity and Activities of Blood Glutathione Peroxidase and Superoxide Dismutase Determined in Healthy Dogs by Using Commercially Available Kits

Plasma Total Antioxidant Capacity and Activities of Blood Glutathione Peroxidase and Superoxide Dismutase Determined in Healthy Dogs by Using Commercially Available Kits

Catalase and Glutathione Peroxidase in Dogs Naturally Infected by Leishmania infantum

Superoxide Dismutase and Glutathione Peroxidase in Dogs with Leishmaniasis Following Antimoniate Therapy

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