Super Wide-Field Photoacoustic Microscopy of Animals and Humans In Vivo

Thao

et al. 2020

Sensors

Self Cite

Correct guiding of the catheter is a critical issue in almost all balloon catheter applications, including arterial stenosis expansion, coronary arterial diseases, and gastrointestinal tracking. To achieve safe and precise guiding of the balloon catheter, a novel imaging method with high-resolution, sufficient depth of penetration, and real-time display is required. Here, we present a new balloon catheter guiding method using fast photoacoustic microscopy (PAM) technique for precise balloon catheter tracking and visualization as a feasibility study. We implemented ex vivo and in vivo experiments with three different medium conditions of balloon catheter: no air, air, and water. Acquired cross-sectional, maximum amplitude projection (MAP), and volumetric 3D PAM images demonstrated its capability as a new imaging guiding tool for balloon catheter tracking and visualization.

Section: Introductionmentioning

confidence: 99%

Feasibility Study of Precise Balloon Catheter Tracking and Visualization with Fast Photoacoustic Microscopy

Thao

et al. 2020

Sensors

Self Cite

Journal of Biophotonics

“…Small animals are essential models for basic biomedical research. Thus, in vivo PAM can directly visualize high‐resolution microvascular networks of small animals, for guiding the basic biomedical research, such as, subcutaneous microvasculature imaging , brain functional imaging , and treatment response monitoring . The vascular network of organ usually requires large field‐of‐view (FOV) imaging .…”

Section: Introductionmentioning

confidence: 99%

“…Wide‐field PAM has been used in many diseases, such as, ischemic stroke , myocardial infraction , burn healing , hemorrhagic shock and vascular malformations . The recording of local dynamics usually uses real‐time imaging . However, the large field of view and real‐time imaging are often two conflicting goals, which limit the PAM system to achieve a fast transition from whole to local imaging of small animal organs.…”

Section: Introductionmentioning

confidence: 99%

Wide‐field monitoring and real‐time local recording of microvascular networks on small animals with a dual‐raster‐scanned photoacoustic microscope

Yang

Wang

Zhang

et al. 2020

Abstact Photoacoustic microscopy (PAM) provides a new method for the imaging of small‐animals with high‐contrast and deep‐penetration. However, the established PAM systems have suffered from a limited field‐of‐view or imaging speed, which are difficult to both monitor wide‐field activity of organ and record real‐time change of local tissue. Here, we reported a dual‐raster‐scanned photoacoustic microscope (DRS‐PAM) that integrates a two‐dimensional motorized translation stage for large field‐of‐view imaging and a two‐axis fast galvanometer scanner for real‐time imaging. The DRS‐PAM provides a flexible transition from wide‐field monitoring the vasculature of organs to real‐time imaging of local dynamics. To test the performance of DRS‐PAM, clear characterization of angiogenesis and functional detail was illustrated, hemodynamic activities of vasculature in cerebral cortex of a mouse were investigated. Furthermore, response of tumor to treatment were successfully monitored during treatment. The experimental results demonstrate the DRS‐PAM holds the great potential for biomedical research of basic biology.

“…Previously, works have reported mosaic acquisition methods for conventional photoacoustic systems [18,19]. Shao et al reported a mosaic acquisition method for optical-resolution photoacoustic microscopy with a step size of 1.2 µm.…”

Section: Introductionmentioning

confidence: 99%

Rapid High-Resolution Mosaic Acquisition for Photoacoustic Remote Sensing

Abbasi

Bell

Reza

2020

Sensors

Mechanical stages are routinely used to scan large expanses of biological specimens in photoacoustic imaging. This is primarily due to the limited field of view (FOV) provided by optical scanning. However, stage scanning becomes impractical at higher scanning speeds, or potentially unfeasible with heavier samples. Also, the slow scan-rate of the stages makes high resolution scanning a time-consuming process. Some clinical applications such as microsurgery require submicron resolution in a reflection-mode configuration necessitating a method that can acquire large field of views with a small raster scanning step size. In this study, we describe a method that combines mechanical stages with optical scanning for the rapid acquisition of high-resolution large FOVs. Optical scanning is used to acquire small frames in a two-dimensional grid formed by the mechanical stages. These frames are captured with specific overlap for effective image registration. Using a step size of 200 nm, we demonstrate mosaics of carbon fiber networks with FOVs of 0.8 × 0.8 mm2 captured in under 70 s with 1.2 µm image resolution. Larger mosaics yielding an imaging area of 3 × 3 mm2 are also shown. The method is validated by imaging a 1 × 1 mm2 section of unstained histopathological human tissue.