Süper kritik CO2 özütlemesi ile iğde çekirdeklerinden quercetin miktarının yüzey cevap metodu ile optimizasyonu

Abstract

In this study, extract of elaeagnus angistofolia seed was obtained by supercritical carbon dioxide solvent method and measured the amount of quercetin by using HPLC equipped with UV detector. Experimental results were analyzed by using "Design Expert 7.0 (demo)" program. Increasing temperature and pressure at about extraction conditions caused a significant increase of quercetin amount. Particle size and extraction period parameters was studied parametrically at the start of this study and identified these parameters according to maximize amount of quercetin. After parametric experiments, the small particle size 0.5 mm and experiment period 120 min were found suitable for this study. After identification of these parameters, experimental design conditions identified by response surface design method were used to optimize pressure and temperature, we optimized temperature and pressure parameters as 65°C and 311 bar to maximize quercetin amount. Maximum oil amount was measured 48.68 mg/g sample. After checking experiments of optimum conditions provided by design method, quercetin amount was found experimentally 9.44 µg quercetin /100 g sample, it was considerably approximate value and favourably to model for estimated 9.49 µg quercetin /100 g sample by Design Expert 7.0 (demo) program.

Extraction and quantification of some valuable flavonoids from pinecone of Pinus brutia via Soxhlet and Supercritical CO2 extraction: a comparison study

“…The optimal temperature and dynamic extraction time of Sc-CO 2 extraction of taxifolin from Pinus nigra bark were identified as 43 °C and 138 min, respectively (Ghoreishi et al 2016a). The optimal CO 2 flow rate and Sc-CO 2 extraction time of quercetin from elaeagnus seeds were identified as 5 l/ min CO 2 and 120 min, respectively (Nuralin et al 2017). The process parameters of Pinus brutia pinecone Sc-CO 2 extraction were selected as pressure, temperature, mean particle size, CO 2 flow rate, co-solvent ratio, and extraction period.…”

“…Similar results have been reported about particle size effect on the extraction yield in the literature. With the effect of the decrease in particle size, 105% oil content and 73.3% quercetin increments were measured in Sc-CO 2 extraction of elaeagnus seeds (Nuralin et al 2017). In this step, the optimum working condition for both the amount of oil and QRK was found as 0.30 mm particle size.…”