Berberis Vulgaris Fruit: Determination of Phenolic Compounds in Extracts Obtained by Supercritical CO2 and Soxhlet Methods Using HPLC

Nuralın, Levent; Gürü, Metin

doi:10.1007/s12161-021-02136-8

Cited by 14 publications

(4 citation statements)

References 25 publications

(9 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Wild edible berries have been consumed as nutritional supplements since ancient times (13) and are important sources of polyphenols (e.g., tannins, flavonoids, phenolic acids, and anthocyanins). Recent investigations on important nutraceutical polyphenolic compounds have attracted intense attention, as in the case of Berberis vulgaris, an important source of polyphenolic molecules (14).…”

Section: Mm) (3)mentioning

confidence: 99%

Total phenolic, monomeric anthocyanin content and antioxidant activity of Berberis commutata Eichler fruits

Jiménez

2023

Vitae

View full text Add to dashboard Cite

Background: Berberis commutata Eichler is a berry that grows in the Peruvian Andes and has been consumed in the Andes of South America since ancient times. The edible fruits have an intense purple color and are rich in anthocyanins and phenolic compounds that are available from February until May each year. The color of the fruits is a soft purple dye for natural fibers, and many birds use them as food. Objective: This study quantified the total phenolic, monomeric anthocyanin content and antioxidant activity of Berberis commutata Eichler berries. Methods: The total phenolic content was determined by the Folin-Ciocalteu colorimetric assay. Monomeric anthocyanin content was determined by the pH difference method, and the antioxidant activity was measured using the Brand-Williams method. Results: The total phenolic content was 7,490 ± 0.85 mg GAE/100g, and the monomeric anthocyanin content was 70 ± 0.03 mg/100g. The antioxidant activity of the berries showed a tendency to increase with B. commutata extract concentration; an EC50 of 0.91 mg/mL was calculated, indicating a high antioxidant power. Conclusion: Our results showed that B. commutata E. has both high total phenolic content and monomeric anthocyanins comparable to other superfruits and increased antioxidant activity, which means that it is possible to use this berberis species as a functional food.

show abstract

Section: Mm) (3)mentioning

confidence: 99%

Total phenolic, monomeric anthocyanin content and antioxidant activity of Berberis commutata Eichler fruits

Jiménez

2023

Vitae

View full text Add to dashboard Cite

show abstract

“…A study by Nuralin and Gürü 7 conducted a supercritical carbon dioxide extraction of compounds from the fruit of Berberis vulgaris and identified antioxidants rutin and apigenin. Rutin was suggested to be a good treatment compound for COVID-19.…”

Section: Introductionmentioning

confidence: 99%

An Analysis in Identifying the Mechanism of Action of the Alkaloids Found in Berberis vulgaris on the Treatment and Prevention of SARS-CoV-2

Gildenhuys,

Steenkamp,

Sekele

et al. 2023

Natural Product Communications

View full text Add to dashboard Cite

Introduction Berberis vulgaris extract is a well-known herbal remedy for treating numerous conditions including coronavirus disease 2019 (COVID-19). The HAB4a method of preparing the extract is a common method used in clinical practice and a further aim of this study was to identify the key alkaloid compounds that are present in an alcohol versus a glycerine-based extraction and determine the mechanism by which these prevent and treat COVID-19. Results and Discussion Identified alkaloid compounds included bervulcine, vulracine, berberrubine, lambertine, berbidine, obamegine, berbamine, berberine, and palmatine in the alcohol-based extract. The glycerine-based extract contained all except bervulcine, vulracine, and berbidine. Berberine and palmatine were the most abundant compounds in both extracts but more berberine was present in the alcohol-based extract and more palmatine in the glycerine-based extraction. Databases revealed compound-gene interactions with human targets involved in signalling and gene regulation networks that could synergistically change cell function. The SARS-CoV-2 spike 1 protein purification yielded about 5 mg of highly purified protein (˃95%) from 1 L of bacterial culture. Isothermal titration calorimetry (ITC) analysis of any potential interaction of palmatine and berberine with the purified spike 1 protein was negative. Thus, it is possible the extract components work in a synergistic manner to prevent and treat COVID-19. Conclusions Berberis vulgaris root extract alkaloid components differ when alcohol or glycerine is used. Berberine and palmatine are the most abundant alkaloids but differ in the amount relative to each other in the extracts. The extract components could affect changes in a variety of human genes. Materials and Methods Ultra-High-Performance Liquid Chromatography—High-Definition quadrupole time-of-flight Mass Spectrometry was used to identify alkaloids. Databases were used to determine the genes that interact with the identified compounds. SARS-CoV-2 spike protein was expressed and purified. Purified protein was used in ITC with berberine and palmatine.

show abstract

“…There are different techniques to extract bioactive compounds. However, conventional methods of extraction such Soxhlet and maceration present several disadvantages such as the use of high temperature (>60 • C), high solvent consumption, and longer extraction times (several hours) [12,13]. Currently, these problems are being solved with green techniques which are more sustainable and careful with the environment such as ultrasound-assisted extraction (UAE) [12], microwave-assisted extraction (MAE) [14], supercritical fluid extraction (SFE) [15], and pressurized-assisted extraction (PLE) [16].…”

Section: Introductionmentioning

confidence: 99%

Comparison between Ultrasonic Bath and Sonotrode Extraction of Phenolic Compounds from Mango Peel By-Products

et al. 2022

View full text Add to dashboard Cite

Phenolic compounds present in mango peel byproducts have been reported to have several beneficial health properties. In this study, we carried out an optimization of phenolic compounds using ultrasound-assisted extraction via ultrasonic bath and sonotrode. To optimize the variables of extraction, a Box–Behnken design was used to evaluate the best conditions to obtain high total phenolic compound extraction and high antioxidant activity evaluated by different methods (DPPH, ABTS, and FRAP). The optimal ultrasonic bath conditions were 45% ethanol, 60 min, and 1/450 ratio sample/solvent (w/v) whereas optimal sonotrode conditions were 55% ethanol, 18 min, and 65% amplitude. The extracts obtained at the optimal conditions were characterized by HPLC–ESI-TOF-MS. A total of 35 phenolic compounds were determined and, to our knowledge, several of them were tentatively identified for the first time in mango peel. The samples were composed mainly by phenolic acids derivatives, specifically of galloylglucose and methylgallate, which represented more than 50% of phenolic compounds of mango peel byproducts. In conclusion, sonotrode is a valuable green technology able to produce enriched phenolic compound extracts from mango peel byproducts that could be used for food, nutraceutical, and cosmeceutical applications.

show abstract

Berberis Vulgaris Fruit: Determination of Phenolic Compounds in Extracts Obtained by Supercritical CO2 and Soxhlet Methods Using HPLC

Cited by 14 publications

References 25 publications

Total phenolic, monomeric anthocyanin content and antioxidant activity of Berberis commutata Eichler fruits

Total phenolic, monomeric anthocyanin content and antioxidant activity of Berberis commutata Eichler fruits

An Analysis in Identifying the Mechanism of Action of the Alkaloids Found in Berberis vulgaris on the Treatment and Prevention of SARS-CoV-2

Comparison between Ultrasonic Bath and Sonotrode Extraction of Phenolic Compounds from Mango Peel By-Products

Contact Info

Product

Resources

About