In this study, extract of elaeagnus angistofolia seed was obtained by supercritical carbon dioxide solvent method and measured the amount of quercetin by using HPLC equipped with UV detector. Experimental results were analyzed by using "Design Expert 7.0 (demo)" program. Increasing temperature and pressure at about extraction conditions caused a significant increase of quercetin amount. Particle size and extraction period parameters was studied parametrically at the start of this study and identified these parameters according to maximize amount of quercetin. After parametric experiments, the small particle size 0.5 mm and experiment period 120 min were found suitable for this study. After identification of these parameters, experimental design conditions identified by response surface design method were used to optimize pressure and temperature, we optimized temperature and pressure parameters as 65°C and 311 bar to maximize quercetin amount. Maximum oil amount was measured 48.68 mg/g sample. After checking experiments of optimum conditions provided by design method, quercetin amount was found experimentally 9.44 µg quercetin /100 g sample, it was considerably approximate value and favourably to model for estimated 9.49 µg quercetin /100 g sample by Design Expert 7.0 (demo) program.
In this work, the dependence of the ethanol production using Saccharomyces cerevisiae 251TP(3-2) on calcium, sodium, and magnesium ion concentration and interaction effects were studied with the use of a statistical experimental design. The parameters of the ethanol concentration model proposed on the basis of Box-Wilson experimental design method were evaluated with the use of the experimental data. Comparison of the predicted values from the model with the experimentally observed values showed that the model is a good fit. From the analysis of model equation, it was seen that sodium ion concentration has significant main effects on ethanol production, and there is interactive effect only between calcium and magnesium. With the use of developed model, maximum ethanol concentration of 3.73% (v/v) was obtained when calcium, sodium, and magnesium concentration were 1,515, 930, and 128 mg/L, respectively, for the 10% sugar concentration in synthetic molasses.
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