Sunxiuqinia elliptica gen. nov., sp. nov., a member of the phylum Bacteroidetes isolated from sediment in a sea cucumber farm

Abstract

Sunxiuqinia elliptica gen. nov., sp. nov., a member of the phylum Bacteroidetes isolated from sediment in a sea cucumber farm Three novel aerobic, elliptic bacteria, designated DQHS4 T , DQHS8 and DQHS15, were isolated from sediment of a seashore pond for sea cucumber culture in Jimo, Qingdao, on the east coast of China. Cells were Gram-, oxidase-and catalase-negative. All three strains grew at 15-42 6C, pH 5-9 and NaCl concentrations between 0.5 and 10 %. DNA-DNA hybridization experiments revealed high (.85 %) relatedness among the three novel isolates and suggested that the strains constitute a single species. Comparative 16S rRNA gene sequence analysis indicated that these bacteria had less than 90 % similarity to all described species of the phylum Bacteroidetes; the closest relative of the three isolates was Prolixibacter bellariivorans F2 T , sharing only 89.6 % sequence similarity. The major cellular fatty acids were iso-C 17 : 0 3-OH (19.8-20.0 %), iso-C 15 : 0 (16.9-17.3 %), anteiso-C 17 : 1 B and/or iso-C 17 : 1 I (7.4-8.7 %), C 17 : 0 2-OH (8.4 %), anteiso-C 15 : 0 (8.2-8.6 %) and C 17 : 1 v6c (5.6-6.0 %). The major respiratory quinone was menaquinone-7 (MK-7) and the DNA G+C content was 41.8-43.5 mol%. Based on the distinct phylogenetic position and the combination of genotypic, phenotypic and chemotaxonomic characteristics, these three strains were considered to represent a novel species of a new genus in the phylum Bacteroidetes, for which the name Sunxiuqinia elliptica gen. nov., sp. nov. is proposed. The type strain of Sunxiuqinia elliptica is DQHS4 T (5CGMCC 1.9156 T 5NCCB 100301 T 5LMG 25367 T ).

Mangrovibacterium diazotrophicum gen. nov., sp. nov., a nitrogen-fixing bacterium isolated from a mangrove sediment, and proposal of Prolixibacteraceae fam. nov.

“…T shared less than 93 % similarity with its closest relatives S. elliptica DQHS4 T (Qu et al, 2011) and Sunxiuqinia faeciviva JAM-BA0302…”

“…T . Data were obtained in this study except for the fatty acid profiles of S. elliptica DQHS4 T and P. bellariivorans JCM 13498 T , which were taken from Qu et al (2011) and Holmes et al (2007), respectively. Only fatty acids present at .1 % are shown.…”

Sunxiuqinia rutila sp. nov., a new member of the phylum Bacteroidetes isolated from marine sediment

“…Strains: 1, HG677 T (Sunxiuqinia rutila sp. nov.); 2, Sunxiuqinia elliptica DQHS4 T (Qu et al, 2011); 3, Sunxiuqinia elliptica DQHS8 (Qu et al, 2011); 4, Sunxiuqinia elliptica DQHS15 (Qu et al, 2011); 5, Sunxiuqinia faeciviva JAM-BA0302…”

“…Strains: 1, HG677 T (Sunxiuqinia rutila sp. nov.); 2, Sunxiuqinia elliptica DQHS4T (Qu et al, 2011;Takai et al, 2013); 3, Sunxiuqinia faeciviva JAM-BA0302…”

“…T (Qu et al, 2011;Takai et al, 2013). Symbols: GL, glycolipid; PE, phosphatidylethanolamine; UL, unidentified lipid; UAL, unidentified aminolipid; UPL, unidentified phospholipid; +, positive; , negative; ND, no data available.…”