Sunlight mediated green synthesis of silver nanoparticles using Polygonatum graminifolium leaf extract and their antibacterial activity

Rawat, Vanshika; Sharma, Anjna; Bhatt, Vijay Prasad; Singh, Raghvendra Pratap; Maurya, Indresh Kumar

doi:10.1016/j.matpr.2020.05.274

Cited by 29 publications

(19 citation statements)

References 20 publications

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“…Presence of sunlight was also helpful for successful and effective green synthesis of ZnONPs. The phyto-molecules present in C. verrucosa extract are generally photosensitive flavoproteins and thus they enhance the reduction of metal ions for the effective synthesis and capping of NPs [51,52]. The color of the solution was turned from pale yellow to white, confirming ZnO NPs synthesis [46].…”

Section: Zno Nps Synthesis and Its Characterizationmentioning

confidence: 94%

Crotalaria verrucosa Leaf Extract Mediated Synthesis of Zinc Oxide Nanoparticles: Assessment of Antimicrobial and Anticancer Activity

et al. 2020

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In this work, we present an ecofriendly, non-hazardous, green synthesis of zinc oxide nanoparticles (ZnO NPs) by leaf extract of Crotalaria verrucosa (C. verrucosa). Total phenolic content, total flavonoid and total protein contents of C. verrucosa were determined. Further, synthesized ZnO NPs was characterized by UV–visible spectroscopy (UV-vis), X-ray diffractometer (XRD), Fourier transform infra-red (FTIR) Spectra, transmission electron microscope (TEM), and Dynamic light scattering (DLS) analysis. UV-vis shows peak at 375 nm which is unique to ZnO NPs. XRD analysis demonstrates the hexagonal phase structures of ZnO NPs. FTIR spectra demonstrates the molecules and bondings associated with the synthesized ZnO NPs and assures the role of phytochemical compounds of C. verrucosa in reduction and capping of ZnO NPs. TEM image exhibits that the prepared ZnO NPs is hexagonal shaped and in size ranged between 16 to 38 nm which is confirmed by DLS. Thermo-gravimetric analysis (TGA) was performed to determine the thermal stability of biosynthesized nanoparticles during calcination. The prepared ZnO NPs showed significant antibacterial potentiality against Gram-positive (S. aureus) and Gram-negative (Proteus vulgaris, Klebsiella pneumoniae, and Escherichia coli) pathogenic bacteria and SEM image shows the generalized mechanism of action in bacterial cell after NPs internalization. In addition, NPs are also found to be effective against the studied cancer cell lines for which cytotoxicity was assessed using MTT assay and results demonstrate highest growth of inhibition at the concentration of 100 µg/mL with IC50 value at 7.07 µg/mL for HeLa and 6.30 µg/mL for DU145 cell lines, in contrast to positive control (C. verrucosa leaf extract) with IC50 of 22.30 µg/mL on HeLa cells and 15.72 µg/mL on DU145 cells. Also, DAPI staining was performed in order to determine the effect on nuclear material due to ZnO NPs treatment in the studied cell lines taking leaf extract as positive control and untreated negative control for comparison. Cell migration assay was evaluated to determine the direct influence of NPs on metastasis that is potential suppression capacity of NPs to tumor cell migration. Outcome of the synthesized ZnO NPs using C. verrucosa shows antimicrobial activity against studied microbes, also cytotoxicity, apoptotic mediated DNA damage and antiproliferative potentiality in the studied carcinoma cells and hence, can be further used in biomedical, pharmaceutical and food processing industries as an effective antimicrobial and anti-cancerous agent.

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Section: Zno Nps Synthesis and Its Characterizationmentioning

confidence: 94%

Crotalaria verrucosa Leaf Extract Mediated Synthesis of Zinc Oxide Nanoparticles: Assessment of Antimicrobial and Anticancer Activity

et al. 2020

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“…Ekennia, Anthony C., et al reported the formation of spherical silver nanoparticles using Euphorbia sanguinea and its photocatalytic degradation of CR (90% within 1 h) [208]. In the period of 2019-2020, several publications reported Silver nanoparticles synthesized using leaf extract of various plants, e.g., Borago officinalis [209], Tragopogon collinus [210], Melia azedarach [211], Mentha aquatica [212], Ziziphus joazeiro [213], Elytraria acaulis [214], Hyptis suaveolens [215], Caesalpinia pulcherrima [216], Gomphrena globosa [217], Plumbago auriculata [218], Cucumis prophetarum [219], Polygonatum graminifolium [220], Cocos nucifera [221], Mimosa albida [222], Capparis zeylanica [223], Holoptelea integrifolia [224], Annona Reticulatal [225], Combretum erythrophyllum [226], Berberis vulgaris [227], Catharanthus roseus [228], Ganonerion polymorphum [229], Premna integrifolia L [230], and Piper betle [231]. Roots are also well established for the synthesis of silver nanoparticles, as shown in Table 13.…”

Section: Synthesis Of Silver Nanoparticles Using Leaf Extracts: (2019-2020)mentioning

confidence: 99%

Green Synthesis of Metal and Metal Oxide Nanoparticles Using Different Plants’ Parts for Antimicrobial Activity and Anticancer Activity: A Review Article

et al. 2021

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Nanotechnology emerged as a scientific innovation in the 21st century. Metallic nanoparticles (metal or metal oxide nanoparticles) have attained remarkable popularity due to their interesting biological, physical, chemical, magnetic, and optical properties. Metal-based nanoparticles can be prepared by utilizing different biological, physical, and chemical methods. The biological method is preferred as it provides a green, simple, facile, ecofriendly, rapid, and cost-effective route for the green synthesis of nanoparticles. Plants have complex phytochemical constituents such as carbohydrates, amino acids, phenolics, flavonoids, terpenoids, and proteins, which can behave as reducing and stabilizing agents. However, the mechanism of green synthesis by using plants is still highly debatable. In this report, we summarized basic principles or mechanisms of green synthesis especially for metal or metal oxide (i.e., ZnO, Au, Ag, and TiO2, Fe, Fe2O3, Cu, CuO, Co) nanoparticles. Finally, we explored the medical applications of plant-based nanoparticles in terms of antibacterial, antifungal, and anticancer activity.

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“…Like 3D response surface plots, 2D contour plots describe the analogues interpretation on relationship between the factors. Thus indicating the importance of the three parameters i) the silver nitrate, the donor of the silver ions for the synthesis [36], ii) the plant extract which is the biological reducing agent due to the presence of the nitrate reductase in synthesizing the silver nanoparticles [37] and iii) sunlight which act as a photo catalyst in the synthesis of silver nanoparticle [38,39]. This method of synthesis of silver nanoparticles through a green approach proves to be much effective and cheap method as it requires the natural plant extract and sunlight as a catalyst.…”

Section: Box Behnken Design (Bbd)mentioning

confidence: 99%

WITHDRAWN: Optimization of process parameters for the green synthesis of silver nanoparticles using Plackett-Burman and 3-level Box–Behnken Design

Halima¹,

Narula²,

Sivakiran³

2020

Preprint

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Background: The present work describes the synthesis of silver nanoparticles (AgNps) from leaf extracts of Piper betle and Jatropha curcas using a green approach. Green synthesis of nanoparticle is superior over other methods of synthesis as it is ecofriendly and cost effective. The phytochemical components in the leaf extract play a vital role in reducing the AgNO3 and hence synthesizing the silver nanoparticles. During this reduction activity, the several factors which affect the synthesis of nanoparticles were investigated and optimized according to the yield of nanoparticles. The experimental conditions investigated were pH, temperature, pressure, time of reaction, microwave radiation, UV radiation and concentration of plant extract, silver nitrate & sunlight. Satisfactory yields of silver nanoparticles synthesis from plant extract were obtained by using optimum conditions when compared to conventional synthesis of nanoparticles. The optimization parameter was later Result: A mathematical model was formulated to correlate the interactive influence of the parameters and the significant reduction. Plackett-Burman design (PBD) indicated that concentration of plant extract, concentration of silver nitrate and sunlight were the major parameters affecting the synthesis of silver nanoparticle. The mutual interactions of these variables are mapped in the design by 3 Box-Behnken design (BBD). The significant factors and their interactions in the green synthesis were examined by analysis of Variance (ANOVA). The result indicated that the BBD was a good predictive model for the experimental results. Though the plant extracts are different, the characterization of the synthesized nanoparticle after optimization of parameters showed uniform size and shape i.e. spherical shape and size of 41 nm. Conclusion: The silver nanoparticles were synthesized under different optimization parameters were uniformly shaped (spherical) and sized (41 nm) particles. The mathematical models Plackett-Burman and Box-Behnken helped in analyzing the impact of the optimization parameters.

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Sunlight mediated green synthesis of silver nanoparticles using Polygonatum graminifolium leaf extract and their antibacterial activity

Cited by 29 publications

References 20 publications

Crotalaria verrucosa Leaf Extract Mediated Synthesis of Zinc Oxide Nanoparticles: Assessment of Antimicrobial and Anticancer Activity

Crotalaria verrucosa Leaf Extract Mediated Synthesis of Zinc Oxide Nanoparticles: Assessment of Antimicrobial and Anticancer Activity

Green Synthesis of Metal and Metal Oxide Nanoparticles Using Different Plants’ Parts for Antimicrobial Activity and Anticancer Activity: A Review Article

WITHDRAWN: Optimization of process parameters for the green synthesis of silver nanoparticles using Plackett-Burman and 3-level Box–Behnken Design

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