Sunflower Seed Lipase: Extraction, Purification, and Characterization

Sagiroglu, Ayten; Arabaci, Nilay

doi:10.1081/pb-200041442

Cited by 28 publications

(14 citation statements)

References 14 publications

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“…An optimum pH at 8.0 has been reported earlier for Bacillus megaterium 20‐1 (Jung et al. 2003) and at pH 7.5 for sunflower seed lipase (Sagiroglu and Arabaci 2005).…”

Section: Resultsmentioning

confidence: 58%

AN ESTEROLYTIC ACTIVITY FROM A WILD EDIBLE MUSHROOM,LYCOPERDON PERLATUM

Çolak

Camedan

Faiz

et al. 2009

Journal of Food Biochemistry

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Lycoperdon perlatum Pers. (Lycoperdaceae, Agaricales, Agaricomycetidae, Agaricomycetes, Basidiomycota, Fungi) was evaluated for its esterolytic potential. Native electrophoresis of the crude extracts showed four bands having Rf values of 0.34, 0.39, 0.52 and 0.59. The esterase showed the highest activity toward a short‐chain substrate, p‐nitrophenyl acetate. Optimum reaction conditions for L. perlatum crude extract were attained at pH 8.0 and 40C. Esterolytic activity of enzyme extract was stimulated in the presence of Mn2+, Fe2+, Ca2+ and Zn2+ in the reaction mixture. The enzyme activity was stimulated by incubation at pH 6.0 but retained 77% of its original activity at its optimum pH after 24 h. Thermal inactivation was displayed after incubation for 20 min at various temperatures above 30C. At 1 mM final concentration, 2‐mercaptoethanol, dithiothreitol, ethylenediamine tetraacetic acid and p‐methylphenyl sulfonylfluoride inhibited the esterolytic reaction. These results support that the crude L. perlatum extract possesses an esterolytic activity having properties similar to other esterases. PRACTICAL APPLICATIONS Esterases catalyzing the cleavage and formation of ester bonds are known α/β‐hydrolases (EC 3.1.1.X). Esterases are used for the synthesis of flavor esters for the food industry, modification of triglycerides for fat and oil industry and resolution of racemic mixtures used for the synthesis of fine chemicals for the pharmaceutical industry. Therefore, the search for new enzyme sources is important for the development of new enzymes and applications.

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“…An optimum pH at 8.0 has been reported earlier for Bacillus megaterium 20‐1 (Jung et al. 2003) and at pH 7.5 for sunflower seed lipase (Sagiroglu and Arabaci 2005).…”

Section: Resultsmentioning

confidence: 58%

AN ESTEROLYTIC ACTIVITY FROM A WILD EDIBLE MUSHROOM,LYCOPERDON PERLATUM

Çolak

Camedan

Faiz

et al. 2009

Journal of Food Biochemistry

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“…Because of their chemical composition and nutritional value sunflower seeds are considered to be a great source of lipids and proteins [2] and are largely used in the production of edible oils and animal feed. The protein content of the seeds is approximately 50-60% [3]. Also, sunflower oil has a high quantity of the natural oxidant vitamin E compared to other edible oils [1,4].…”

Section: Introductionmentioning

confidence: 99%

Extraction of sunflower (Heliantus annuus L.) oil with supercritical CO2 and subcritical propane: Experimental and modeling

Nimet¹,

Silva²,

Palú³

et al. 2011

Chemical Engineering Journal

106

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“…Similarly, corn seed lipase was found to have high activity toward tricaprin and trilinolein and the lupin seed lipase was more active on TAGs containing saturated fatty acids than containing unsaturated fatty acids (Huang et al, 1988 ; Sanz and Olias, 1990 ). Sunflower seed lipase have shown preference for triacylglycerols with mono-unsaturated fatty acids (Sagiroglu and Arabaci, 2005 ). Bean lipases showed specificity for short and medium chain fatty acids (Enujiugha et al, 2004 ).…”

Section: Introductionmentioning

confidence: 99%

Reduced Triacylglycerol Mobilization during Seed Germination and Early Seedling Growth in Arabidopsis Containing Nutritionally Important Polyunsaturated Fatty Acids

et al. 2016

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There are now several examples of plant species engineered to synthesize and accumulate nutritionally important polyunsaturated fatty acids in their seed triacylglycerols (TAG). The utilization of TAG in germinating seeds of such transgenic plants was unknown. In this study, we examined the TAG utilization efficiency during seed germination in transgenic Arabidopsis seeds containing several examples of these fatty acids. Seed TAG species with native fatty acids had higher utilization rate than the TAG species containing transgenically produced polyunsaturated fatty acids. Conversely, quantification of the fatty acid components remaining in the total TAG after early stages of seed germination revealed that the undigested TAGs tended to contain elevated levels of the engineered polyunsaturated fatty acids (PUFA). LC-MS analysis further revealed asymmetrical mobilization rates for the individual TAG species. TAGs which contained multiple PUFA fatty acids were mobilized slower than the species containing single PUFA. The mobilized engineered fatty acids were used in de novo membrane lipid synthesis during seedling development.

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Sunflower Seed Lipase: Extraction, Purification, and Characterization

Cited by 28 publications

References 14 publications

AN ESTEROLYTIC ACTIVITY FROM A WILD EDIBLE MUSHROOM,LYCOPERDON PERLATUM

AN ESTEROLYTIC ACTIVITY FROM A WILD EDIBLE MUSHROOM,LYCOPERDON PERLATUM

Extraction of sunflower (Heliantus annuus L.) oil with supercritical CO2 and subcritical propane: Experimental and modeling

Reduced Triacylglycerol Mobilization during Seed Germination and Early Seedling Growth in Arabidopsis Containing Nutritionally Important Polyunsaturated Fatty Acids

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