Corn poppy (Papaver rhoeas L.) leaf has been extensively used as garniture in salads and drugs in folk medicine. In this study, the possible antioxidant properties of water (WE), ethanol (EE), and acetone (AE) extracts of corn poppy leaves were investigated using different antioxidant tests, including total antioxidant activity in linoleic acid system, DPPH • scavenging activity, reducing power, chelation activity, and hydrogen peroxide scavenging activity. In addition, the amount of total phenolics was also determined. Total antioxidant activities of all extracts were greater than 85% at 400 μg/mL concentration. The scavenging effects of WE and EE on DPPH • radical were found to be 88.46 ± 0.08% and 86.81 ± 0.37% at 800 μg/mL concentration, respectively, which was comparable to standard antioxidants, such as BHA and α-TP. The reducing power of extracts was in the order of WE > EE > AE. The percentage of metal chelating activity of 800 μg/mL concentration of WE was found to be 79.51 ± 4.05%. Our results indicated that the leaves of Papaver rhoeas L. showed the potential to be used as a natural antioxidant.
A simple procedure for the extraction of the lipolytic activity from sunflower seed has been developed. Various conditions of extraction have been optimized in order to obtain maximum yield of lipase. A new lipase enzyme was purified by the fractional salt precipitation from the supernatant, dialysis on a cellulose membrane, and gel column chromatography on Sephadex G-75. The lipase was monomeric, with an apparent Mr of 22 kDa and a pI of 8, with the electrophoretic analysis. Kinetics of the enzyme activity versus substrate concentration showed typical lipase behavior, with Km and Vmax, values of 1.33 mM and 555 U/mg. All triglycerides were efficiently hydrolyzed by the enzyme, but this showed a preference towards triglycerides of natural mono unsaturated fatty acids. The optimum temperature, pH, and incubation time for lipolytic activity were 50 degrees C, 7.5, and 5 min, respectively. The stability of the sunflower lipase was investigated under operational and storage conditions. It was found that this enzyme preserved its lipolytic activity at temperatures between at 35-50 degrees C, alkaline pH, and for a period of about four months.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.