Sulforaphane Inhibits the Expression of Long Noncoding RNA H19 and Its Target APOBEC3G and Thereby Pancreatic Cancer Progression

Luo, Yiqiao; Yan, Bin; Liu, Li; Yin, Libo; Ji, Huihui; An, Xuefeng; Gladkich, Jury; Qi, Zhimin; Torre, Carolina De La; Herr, Ingrid

doi:10.3390/cancers13040827

Cited by 18 publications

(17 citation statements)

References 67 publications

(102 reference statements)

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“…↓ BRAF-activated long noncoding RNA (BANCR) luteolin thyroid carcinoma [182] long non-coding RNA epigallocatechin-3-gallate lung cancer [183] ↓ lnc RNA LINC00511 gastric cancer [184] ↓ lncRNAs H19 sulforaphane pancreatic ductal adenocarcinoma [185] ↓ lncRNA LUCAT1 vitamin D oral squamous cell carcinoma [186] ↓ lncRNA CCAT2 ovarian cancer [187] ↑ lncRNA MEG3 colorectal cancer [188]…”

Section: Mechanisms Bioactive Component Disorders Referencesmentioning

confidence: 99%

Influence of the Bioactive Diet Components on the Gene Expression Regulation

et al. 2021

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Diet bioactive components, in the concept of nutrigenetics and nutrigenomics, consist of food constituents, which can transfer information from the external environment and influence gene expression in the cell and thus the function of the whole organism. It is crucial to regard food not only as the source of energy and basic nutriments, crucial for living and organism development, but also as the factor influencing health/disease, biochemical mechanisms, and activation of biochemical pathways. Bioactive components of the diet regulate gene expression through changes in the chromatin structure (including DNA methylation and histone modification), non-coding RNA, activation of transcription factors by signalling cascades, or direct ligand binding to the nuclear receptors. Analysis of interactions between diet components and human genome structure and gene activity is a modern approach that will help to better understand these relations and will allow designing dietary guidances, which can help maintain good health.

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Section: Mechanisms Bioactive Component Disorders Referencesmentioning

confidence: 99%

Influence of the Bioactive Diet Components on the Gene Expression Regulation

et al. 2021

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“…Recently, the lncRNA H19 (H19) has been identified as a cancer promotor in different cancer types (6)(7)(8)(9). H19 is highly expressed in PDAC, and it promotes proliferation, migration, and metastasis (6,(10)(11)(12). We identified the innate anti-viral immunity gene APOBEC3G as a major H19 downstream gene (12,13) and demonstrated that the downregulation of H19 or APOBEC3G by siRNA or the bioactive agent sulforaphane prevented H19-mediated PDAC progression features as demonstrated by assays for colony formation, migration, invasion, Smad2 phosphorylation and tumor xenograft growth (12).…”

Section: Introductionmentioning

confidence: 99%

UHMK1 Is a Novel Marker for Personalized Prediction of Pancreatic Cancer Prognosis

et al. 2022

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Pancreatic ductal adenocarcinoma (PDAC) is among the leading causes of cancer mortality, and new therapeutic options are urgently needed. Long noncoding RNA H19 (H19) is known to promote PDAC progression, but the downstream genes of H19 are largely unknown. Five PDAC cell lines, nonmalignant pancreatic cells, TCGA, GEO-derived pancreatic tissues (malignant, n=413; nonmalignant, n=234), a pancreatic tissue array (n=96), and pancreatic tissues from our clinic (malignant, n=20; nonmalignant, n=20) were examined by a gene array, RT-qPCR, Western blotting, MTT, colony formation, wound-healing, siRNA-mediated gene silencing, bioinformatics, xenotransplantation, and immunohistochemistry assays. The cell cycle inhibitor, UHMK1, was identified to have the strongest correlation with H19. UHMK1 expression was enhanced in PDAC, and high UHMK1 expression correlated with tumor stage, and lower overall survival. siRNA-mediated UHMK1 downregulation inhibited progression signaling. siRNA-mediated downregulation of H19 or UHMK1 inhibited tumor proliferation and xenograft growth. Based on the correlation between UHMK1 expression and clinical parameters, we developed a nomogram that reliably predicts patient prognosis and overall survival. Together, we characterized UHMK1 as an H19-induced oncogene and verified it as a novel PDAC prognostic marker for overall survival.

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“…Finally, we verified our concept in vivo by the use of tumor xenotransplantation to fertilized chicken eggs. This avian model is an excellent alternative to experiments performed on mammals, such as mice [ 63 ], and has been evaluated in several of our recent studies [ 64 , 65 ]. One major advantage of the chicken egg model is its natural immunodeficiency because immunity occurs only after hatching [ 66 ].…”

Section: Discussionmentioning

confidence: 99%

Alpha-Lipoic Acid Prevents Side Effects of Therapeutic Nanosilver without Compromising Cytotoxicity in Experimental Pancreatic Cancer

Liu

Schaefer

et al. 2021

Cancers

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Silver nanoparticles (AgNPs) have attracted attention in cancer therapy and might support the treatment of pancreatic ductal adenocarcinoma (PDAC). Silver is in clinical use in wound dressings, catheters, stents and implants. However, the side effects of systemic AgNP treatment due to silver accumulation limit its therapeutic application. We evaluated whether the antioxidant and natural agent α-lipoic acid might prevent these side effects. We synthesized AgNPs using an Ionic-Pulser® Pro silver generator and determined the concentration by inductively coupled plasma–optical emission spectrometry. The effect of α-lipoic acid was examined in four PDAC and two nonmalignant cell lines by MTT, FACS analysis, TEM, xenotransplantation and immunohistochemistry. The viability of PDAC cells was nearly totally abolished by AgNP treatment, whereas nonmalignant cells largely resisted. α-Lipoic acid prevented AgNP-induced cytotoxicity in nonmalignant cells but not in PDAC cells, which might be due to the higher sensitivity of malignant cells to silver-induced cytotoxicity. α-Lipoic acid protected mitochondria from AgNP-induced damage and led to precipitation of AgNPs. AgNPs reduced the growth of tumor xenografts, and cotreatment with α-lipoic acid protected chick embryos from AgNP-induced liver damage. Together, α-lipoic acid strongly reduced AgNP-induced side effects without weakening the therapeutic efficacy.

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Sulforaphane Inhibits the Expression of Long Noncoding RNA H19 and Its Target APOBEC3G and Thereby Pancreatic Cancer Progression

Cited by 18 publications

References 67 publications

Influence of the Bioactive Diet Components on the Gene Expression Regulation

Influence of the Bioactive Diet Components on the Gene Expression Regulation

UHMK1 Is a Novel Marker for Personalized Prediction of Pancreatic Cancer Prognosis

Alpha-Lipoic Acid Prevents Side Effects of Therapeutic Nanosilver without Compromising Cytotoxicity in Experimental Pancreatic Cancer

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