Sulfonamide Resistance Gene in a Transferable R Plasmid of Pasteurella piscicida

Abstract

The sulfonamide resistance (SAr) determinant was cloned from a transferable R plasmid of Pasteurella piscicida, pSP9351, and the sequence was determined. The resistance gene (pp-sul) was localized to an approximately 1-kb region that includes the PstI-EcoRI site in the restriction map. An open reading frame coding a sul II-type gene composed of 810 nucleotides was identified. A direct repeat sequence was shown in the 5' flanking region of pp-sul, and a plasmid recombinational event may have occurred during the construction of pSP9351. In the 3' flanking region of the gene, a sequence homologous to the 5' noncoding sequence of the trimethoprim resistance gene, dhfr IX was found.

Molecular diversity of Photobacterium damselae ssp. piscicida from cultured amberjacks (Seriola spp.) in Japan by pulsed-field gel electrophoresis and plasmid profiles

“…In the previous reports of P. damselae ssp. piscicida, resistance genes derived from transferable R plasmids were reported against the chloramphenicol (Kim and Aoki 1993b;Morii et al 2003), sulfonamide (Kim and Aoki 1996), tetracycline and kanamycin (Kim and Aoki 1994). On the other hand, Kim et al (2005) reported that single mutation in the gyrA gene (at Ser-83) caused a quinolone (oxolinic acid, nalidixic acid and orbifloxacin) resistance in P. damselae ssp.…”

Complete DNA Sequence and Analysis of the Transferable Multiple-Drug Resistance Plasmids (R Plasmids) from Photobacterium damselae subsp. piscicida Isolates Collected in Japan and the United States

“…piscicida, the Km r , Tc r , and Cp r genes were flanked by IS26, making them class I transposons, while the Sa r and Tmp r genes were bordered by transposase genes, suggesting that they are class II transposons. The genetic element of a similar, previously described plasmid, pSP9351 (with the structure IS26-Km r gene-IS26-Tc r gene-IS26-Sa r gene-IS26), is regarded as a complex transposon and exhibits a functional hybrid promoter made up of the transposable element IS26 (6,8,9). Direct evidence shows that IS26 provides part of the promoter of the antibiotic resistance operon (IAB) of pBWH77 and has an active role in the evolution and mobility of antibiotic resistance genes (10).…”

Sequence Analysis of the Florfenicol Resistance Gene Encoded in the Transferable R‐Plasmid of a Fish Pathogen, Pasteurella piscicida

“…1) were identical to the downstream sequence of the sulfonamide resistance gene in the complementary strand of pPS1 (8). Within the 903-nucleotide sequence, a 231-nucleotide sequence between PstI and BamHI sites indicated by Si was observed in the upstream region of the trimethoprim resistance gene, dhfrlX (7) (Figs.…”

“…In the present study, the FF resistance determinations were analyzed by cloning and nucleotide sequencing. We determined the nucleotide sequence of the gene and the regions adjacent to it, and speculated on the insert region of the FF resistance gene in the transferable Rplasmid of P. piscicida (7,8). Sequencing kit with T7 polymerase (Pharmacia, U.S.A.).…”

“…We determined the nucleotide sequence of the gene and the regions adjacent to it, and speculated on the insert region of the FF resistance gene in the transferable Rplasmid of P. piscicida (7,8 Sequencing kit with T7 polymerase (Pharmacia, U.S.A.). The nucleotide sequence and the deduced amino acid sequence of the FF resistance gene were analyzed using the Genetic Bio database software (Genetyx, SDC, Tokyo), and compared with the DNA sequences previously reported and deposited in the DNA database.…”