Sequence Analysis of the Florfenicol Resistance Gene Encoded in the Transferable R‐Plasmid of a Fish Pathogen, <i>Pasteurella piscicida</i>

Kim, Eun-Heui; Aoki, Takashi

doi:10.1111/j.1348-0421.1996.tb01125.x

Cited by 113 publications

(78 citation statements)

References 14 publications

(21 reference statements)

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“…respiratory pasteurellosis and the floR resistance gene has been located on large conjugative plasmids in the fish pathogen Photobacterium damselae subsp. piscicida [46], and in clinical E. coli strains isolated from poultry and cattle [16,45]. These observations argue for an independent rapid spread of florfenicol resistance by horizontal transfer of the conjugative plasmid carrying the floR gene.…”

Section: Clustering Of Resistance Genesmentioning

confidence: 83%

Importance of integrons in the diffusion of resistance

2001

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Summary -Horizontal transfer of resistance genes is a successful mechanism for the transmission and dissemination of multiple drug resistance among bacterial pathogens. The impact of horizontally transmitted genetic determinants in the evolution of resistance is particularly evident when resistance genes are physically associated in clusters and transferred en bloc to the recipient cell. Recent advances in the molecular characterisation of antibiotic resistance mechanisms have highlighted the existence of genetic structures, called integrons, involved in the acquisition of resistance genes. These DNA elements have frequently been reported in multi-drug resistant strains isolated from animals and humans, and are located either on the bacterial chromosome or on broad-host-range plasmids. The role of integrons in the development of multiple resistance relies on their unique capacity to cluster and express drug resistance genes. Moreover, the spread of resistance genes among different replicons and their exchange between plasmid and bacterial chromosome are facilitated by the integration of integrons into transposable elements. The association of a highly efficient gene capture and expression system, together with the capacity for vertical and horizontal transmission of resistance genes represents a powerful weapon used by bacteria to combat the assault of antibiotics. antimicrobial resistance / integron / transposon / horizontal gene transferRésumé -Importance des intégrons dans la diffusion de la résistance. Le transfert horizontal de gènes est un mécanisme efficace pour la transmission et la dissémination, parmi les agents pathogènes bactériens, de résistances multiples aux antibiotiques. L'impact des déterminants génétiques transmis horizontalement dans l'évolution de la résistance est particulièrement évident lorsque les gènes de résistance sont physiquement regroupés, et transférés en bloc à la cellule réceptrice. Des avancées récentes dans la caractérisation moléculaire des mécanismes de résistance aux antibiotiques ont mis en évidence l'existence de structures génétiques, appelées intégrons, impliqués dans l'acquisition de gènes de résistance. Ces éléments d'ADN ont été fréquemment observés dans des souches bactériennes multi-résistantes isolées chez des animaux ou chez des humains, localisés soit sur le chromosome bactérien, soit sur des plasmides à large spectre d'hôte. Le rôle des intégrons dans le développement de la multi-résistance repose sur leur capacité unique à former des clusters de gènes et à exprimer des gènes Vet. Res. 32 (2001) 243-259 243

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Section: Clustering Of Resistance Genesmentioning

confidence: 83%

Importance of integrons in the diffusion of resistance

2001

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“…A floR homolog was first identified on a plasmid in Pasteurella piscicida recently renamed Photobacterium damselae, a fish pathogen [80]. In addition, the class G tetracycline resistance gene associated with the floR gene in the S. Typhimurium DT104 antibiotic resistance gene cluster was first identified in Vibrio anguillarum, also a fish pathogen [157].…”

Section: Origin Of the Sgi1 Antibiotic Resistance Gene Clustermentioning

confidence: 99%

Emergence of Salmonella epidemics: The problems related to Salmonella enterica serotyp Enteritidis and multiple antibiotic resistance in other major serotypes

Cloeckaert²,

2005

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-Two major changes in the epidemiology of salmonellosis occurred in the second half of the 20th century: the emergence of food-borne human infections caused by S. Enteritidis and by multiple-antibiotic resistant strains of Salmonella. This review updates information on the S. Enteritidis pandemic and focuses on the emergence of Salmonella, carrying the SGI1 antibiotic resistance gene cluster, resistant to extended-spectrum cephalosporins, or resistant to fluoroquinolones. The factors responsible for the emergence of these Salmonella strains could be either of human origin or related to bacterial genome evolution. However, our increasing understanding of the molecular fluidity of the genome shows that any attempt to counteract bacteria results in further bacterial evolution or adaptation of other bacteria to take place in the new free ecological niche. In these conditions, we can ask who is faster: humans who want to eliminate bacterial pathogens or bacteria that continuously evolve to gain new niches.

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“…Genes, such as pp-flo and floR -coding for membrane-associated efflux systems which export chloramphenicol and florfenicol -have been detected on a plasmid in Photobacterium damselae subsp. piscicida [33] as part of a chromosomal multiresistance gene cluster in Salmonella enterica serovars Typhimurium [3,8] and Agona [18], but also on multiresistance plasmids of E. coli [17,32,77].…”

Section: Resistance To Chloramphenicol and Florfenicolmentioning

confidence: 99%

Use of antimicrobials in veterinary medicine and mechanisms of resistance

2001

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-This review deals with the application of antimicrobial agents in veterinary medicine and food animal production and the possible consequences arising from the widespread and multipurpose use of antimicrobials. The various mechanisms that bacteria have developed to escape the inhibitory effects of the antimicrobials most frequently used in the veterinary field are reported in detail. Resistance of bacteria to tetracyclines, macrolide-lincosamide-streptogramin antibiotics, β-lactam antibiotics, aminoglycosides, sulfonamides, trimethoprim, fluoroquinolones and chloramphenicol/florfenicol is described with regard to enzymatic inactivation, decreased intracellular drug accumulation and modification/protection/replacement of the target sites. In addition, basic information is given about mobile genetic elements which carry the respective resistance genes, such as plasmids, transposons, and gene cassettes/integrons, and their ways of spreading via conjugation, mobilisation, transduction, and transformation.antibiotic therapy / growth promotion / resistance mechanism / resistance gene / gene transfer Résumé -Utilisation d'agents antimicrobiens en médecine vétérinaire et mécanismes de résis-tance. Cette revue présente les différents buts pour lesquels les agents antimicrobiens sont utilisés en médecine vétérinaire, dans les élevages d'animaux entrant dans la chaîne alimentaire et les possibles conséquences de cette large utilisation. Une synthèse est faite des différents mécanismes de résis-tance développés par les bactéries, comme l'inactivation enzymatique, la diminution de la concentration intracellulaire de l'antibiotique, les modification/protection/déplacement de cible, qui permettent d'échapper à l'action des antibiotiques les plus fréquemment utilisés dans le domaine vétérinaire :

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Sequence Analysis of the Florfenicol Resistance Gene Encoded in the Transferable R‐Plasmid of a Fish Pathogen, Pasteurella piscicida

Cited by 113 publications

References 14 publications

Importance of integrons in the diffusion of resistance

Importance of integrons in the diffusion of resistance

Emergence of Salmonella epidemics: The problems related to Salmonella enterica serotyp Enteritidis and multiple antibiotic resistance in other major serotypes

Use of antimicrobials in veterinary medicine and mechanisms of resistance

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