Sulfate-reducing bacteria of human intestine. I. Dissimilatory sulfate reduction

Kushkevych, Ivan

doi:10.30970/sbi.0601.181

Cited by 15 publications

(84 citation statements)

References 81 publications

(182 reference statements)

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“…Sulfatereducing bacteria Desulfovibrio, Desulfomicrobium, Desulfobulbus, Desulfobacter, Desulfomonas and Desulfotomaculum genera make an important component of human intestinal microbiocenosis [2,15,19,21,23]. An increased number of these microorganisms can be found in people with periodontitis, inflammatory bowel diseases, ulcerative colitis, rheumatic diseases and many other diseases [6,8,9,16,18,20,24].…”

Section: Introductionmentioning

confidence: 99%

“…An increased number of these microorganisms can be found in people with periodontitis, inflammatory bowel diseases, ulcerative colitis, rheumatic diseases and many other diseases [6,8,9,16,18,20,24]. Some scientists also suggest that the sulfatereducing bacteria (SRB) might cause of some forms of colon cancer, taking into account that these microorganisms produce hydrogen sulfide affecting the intestinal cells metabolism causing various diseases [6,15,16,22,24,28]. This is the main reason why it is necessary to study SRB in more detail.…”

Section: Introductionmentioning

confidence: 99%

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Identification of sulfate-reducing bacteria strains of human large intestine

Kushkevych

2013

Biol. Stud.

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The sulfate-reducing bacteria have been isolated from human intestine. These bacteria are identified by their morphological, physiological and biochemical characteristics as the Desulfovibrio sp. and Desulfomicrobium sp. according to Bergey's Manual of Determinative Bacteriology. Growth of the isolated strains of bacteria has been studied under various conditions including types of media, effect of oxygen, pH, and tempe rature. Bacterial growth in the modified KravtsovSorokin's medium, and process of dissimilatory sulfate reduction by the selected strains of bacteria have been investiga ted. All obtained bacterial cultures used sulfate and lactate intensively as well as produced hydrogen sulfide and acetate in the medium. The Desulfovibrio sp. strain Vib7 produced the highest concentration (up to 3.23 mM) of hydrogen sulfide, while the bacteria used about 99% of sulfate ions presented in the medium. This strain also produced the highest concentration of acetate ions (up to 15.87 mM) on the fifth day of cultivation. Among the studied Desulfomicrobium sp. strains, the highest concentration of hydrogen sulfide was produced by the Desulfomicrobium sp. strain Rod9, and the highest concentration of acetate ions was produced by the Desulfomicrobium sp. strain Rod4.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Identification of sulfate-reducing bacteria strains of human large intestine

Kushkevych

2013

Biol. Stud.

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“…These data were also consistent to previously obtained results of the qualitative and quantitative changes in the microflora in the rats on the twenty-fifth day. The predominant bacteria of the colon of the first (control) animals group were Bacteroides (N 25 Colonic bacteria produce large quantities of hydrogen sulfide [1,4,8,11]. It is known that sulfide is produced by a group of bacteria, sulfate-reducing bacteria that obtain energy via reactions involving the reduction of sulfate to sulfide [2,3,14,15].…”

Section: Resultsmentioning

confidence: 99%

“…The species of Desulfovibrio and Desulfomicrobium genera oxidize lactate incompletely to acetate [1,5,9]. Lactate oxidation to acetate occurs together with the concurrent reduction of sulfate to sulfide [4,6,10,11]. The presence of lactate and sulfate in the human intestine contributes to the intensive bacteria growth and the accumulation of their final metabolic products, acetate and hydrogen sulfide, which are toxic, mutagenic and cancerogenic to epithelial intestinal cells [2,7,8,13,14].…”

Section: Introductionmentioning

confidence: 99%

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Etiological Role of Sulfate-Reducing Bacteria in the Development of Inflammatory Bowel Diseases and Ulcerative Colitis

Kushkevych¹

2014

AJIDM

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Inflammatory bowel disease and ulcerative colitis are complex multifactorial diseases of unknown etiology. The etiological role of the sulfate-reducing bacteria Desulfovibrio piger Vib-7 and Desulfomicrobium sp. Rod-9 in the development of these inflammatory diseases in rats was studied. Applying sulfate-reducing bacteria, ulcerative colitis in the animals was experimentally induced at the fist time. In total 45 animals in three groups were separated and used in this experiment. Animals in the first group received the standard diet containing a special certified feed for rats and were used as a control. The second group received the standard feed and a dose (1 ml per each day) of modified Kravtsov-Sorokin's liquid medium for the initiation of own potential animal intestinal microflora of sulfate-reducing bacteria. The third group of the animals received the standard feed and dose of a suspension with D. piger Vib-7 and Desulfomicrobium sp. Rod-9 (at a ratio of 1 to 1) in the medium. The changes in the colonic microbiota under these conditions were studied. The bacteria belonging to the normal colonic microbiota were associated with the etiology of inflammatory bowel disease and ulcerative colitis. The concentration of sulfide and acetate in feces from different sections of the large intestine of the rats was determined. The level of ulcerations in the second and third groups of sick animals under the specific conditions was demonstrated. The described experimental results could be particularly useful for the study of inflammatory bowel disease and ulcerative colitis and its therapeutic strategy. These data are also indispensable to apply into mechanistic details that will facilitate better preclinical drug/therapy design to target specific components involved in the disease pathogenesis. Keywords

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