The effects of feeding three natural frozen diets, grass shrimp (Palaemonetes sp.), crayfish (Procambarus clarkii) and fish (Sardina pilchardus) and two semi-humid artificial diets (based on fish or shrimp powder) to the cuttlefish, Sepia officinalis, were analysed. Growth rate and feeding rate [FR; % body weight (BW) day )1 ] and food conversions (FC, %) were determined. Cuttlefish fed shrimp grew larger (3.8% BW day )1 ) and had the highest FC, followed by those fed crayfish, and sardine. The highest FR was obtained for cuttlefish fed crayfish (10.5% BW day )1 ). Although both artificial diets were accepted, none produced growth. Digestive gland-to-body weight ratio (DG/ BW ratio) was calculated for animals fed each diet. A positive correlation (r = 0.94) between cuttlefish ingestion FR and DG weight was obtained. Mortality occurred mainly during the last week, and some cannibalism occurred among cuttlefish fed artificial diets. Finally, lipid composition of diets, DG and mantle of each group were analysed. Sardine diet was characterized by high levels of triacylglycerol (TG), whereas the main difference between shrimp and crayfish was the higher n-3/n-6 ratio found in shrimp. Changes in the lipid composition of DG were related to diet, but did not correlate with growth data. A strong loss of TG in the DG of artificial diets groups was notable. No differences in mantle lipid composition among the natural diets were found, but artificial diet groups showed higher contents of neutral lipids in their mantle respect to natural diets. According to results obtained, crayfish (P. clarkii) could be used as an alternative prey for rearing S. officinalis compared with shrimp. Artificial diets showed the worst effects in growth and mortality as well as the stronger influence on DG and mantle lipid composition of cuttlefish. KEY WORDS
The effects of feeding three natural frozen diets, grass shrimp (Palaemonetes sp.), crayfish (Procambarus clarkii) and fish (Sardina pilchardus) and two semi-humid artificial diets (based on fish powder) to mature cuttlefish, Sepia officinalis, were analysed. Growth and feeding rates (GR and FR, % BW day -1 ), food conversions (FC, %), and total protein and lipid composition of the diets were determined. Digestive gland to body weight ratio and absorption efficiency were calculated for each diet. Cuttlefish fed shrimp and crayfish grew larger (1.5 and 1.1% BW day -1 , respectively) compared to the other diets. Shrimp promoted the highest FC, followed by crayfish, and sardine. The highest FR was obtained for cuttlefish fed crayfish (8.4% BW day -1 ). Although both artificial diets were accepted, none produced growth. A positive correlation (r = 0.96) between cuttlefish ingestion rate and digestive gland weight was obtained. Some cannibalism occurred among cuttlefish fed the artificial diets during the last week of the experiment. According to the results obtained, P. clarkii could be used as an alternative prey to shrimp for rearing adult mature ([50 g) S. officinalis.
INTRODUÇÃOA credibilidade das queixas envolvendo funções cognitivas como a memória encontra-se entre os problemas mais comuns e difíceis de identificar na avaliação neuropsicológica em contexto forense. Neste âmbito, os resultados nos testes são habitualmente considerados como tendo um valor de evidência objectiva da validade dos sintomas cognitivos comunicados (défices ou declínio da memória, atenção, concentração, etc.) e dos seus efeitos funcionais nos comportamentos, desempenhos e actividades da vida diária. Contudo, os baixos resultados nos 209
BackgroundExperimental osteoarthritis entails neuropathic-like changes in dorsal root ganglia (DRG) neurons. Since glial activation has emerged as a key player in nociception, being reported in numerous models of neuropathic pain, we aimed at evaluating if glial cell activation may also occur in the DRG and spinal cord of rats with osteoarthritis induced by intra-articular injection of collagenase.MethodsOsteoarthritis was induced by two injections, separated by three days, of 500 U of type II collagenase into the knee joint of rats. Movement-induced nociception was evaluated by the Knee-Bend and CatWalk tests during the following six weeks. Glial fibrillary acidic protein (GFAP) expression in satellite glial cells of the DRG was assessed by immunofluorescence and Western Blot analysis; the pattern of GFAP and activating transcription factor-3 (ATF-3) expression was also compared through double immunofluorescence analysis. GFAP expression in astrocytes and IBA-1 expression in microglia of the L3–L5 spinal cord segments was assessed by immunohistochemistry and Western Blot analysis. The effect of the intrathecal administration of fluorocitrate, an inhibitor of glial activation, on movement-induced nociception was evaluated six weeks after the first collagenase injection.ResultsGFAP expression in satellite glial cells of collagenase-injected animals was significantly increased six weeks after osteoarthritis induction. Double immunofluorescence showed GFAP upregulation in satellite glial cells surrounding ATF-3-positive neurons. In the spinal cord of collagenase-injected animals, an ipsilateral upregulation of GFAP and IBA-1 was also observed. The inhibition of glial activation with fluorocitrate decreased movement- and loading-induced nociception.ConclusionCollagenase-induced knee osteoarthritis leads to the development of nociception associated with movement of the affected joint and to the activation of glial cells in both the DRG and the spinal cord. Inhibition of glial cell activation by fluorocitrate decreases these osteoarthritis-associated nociceptive behaviours. These results suggest that glial cell activation may play a role in the development of chronic pain in this experimental model of osteoarthritis.
The prevalence of food allergy (FA) has increased too rapidly, possibly due to environmental factors. The guidelines recommend strict allergen avoidance, but FA is still the main cause of anaphylaxis in all age groups. Immunotherapy is the only treatment able to change the course of allergic disease, and oral immunotherapy (OIT) is the more effective route in FA. However, it carries the risk of adverse reactions, including anaphylaxis. To improve OIT safety, adjuvant therapy with the immunoglobulin E (IgE) monoclonal antibody omalizumab has been extensively used. Results suggest particular benefit in patients with high risk of fatal anaphylaxis. An alternative approach is to use omalizumab instead of OIT to prevent severe allergic reactions upon accidental exposure. This paper reviews current evidence regarding IgE‐mediated FA, focusing on natural tolerance and food sensitization acquisition, and on avoidance measures and their limitations.
Left paraduodenal hernia is a rarely found condition in which the small bowel herniates through a congenital peritoneal defect located behind the 4th portion of duodenum. It usually courses with non-specific symptoms and a widely variable clinical presentation which makes preoperative diagnosis very difficult. It has been associated with a high rate of complications, including bowel strangulation and even death. Prompt surgical intervention consisting of intraoperative surgeon's awareness and recognition of this condition are crucial in order to improve postoperative morbidity and mortality. The authors present a case of a 73-year-old man with acute intestinal obstruction as the first presentation of a left paraduodenal hernia.
Summary We report a case of a previously healthy 24-year-old man with a 3-month history of gastrointestinal symptoms during exercise and also few minutes after the ingestion of cow's milk (CMBodybuilding protein supplements and cow's milk allergy in adult Previous presentationThe data reported in this study was presented by Maria João Sousa (co-authors: Ferreira AR, Moreira Silva JP) at the EAACI Congress 2015 in Barcelona (Spain) 6-10 June 2015, as a poster entitled "Adult-onset cow's milk allergy and Bodybuilding: is there a connection?". It was awarded the Poster Prize (Thematic Poster Session 54) and was published in the abstract form in Allergy 2015; 70(Suppl. S101):611; at the 36a Reunião Anual da Sociedade Portuguesa de Alergologia e Imunologia Clínica in Coimbra (Portugal) 9-11 October 2015, as a poster entitled "Alergia às proteínas do leite de vaca: uma apresentação incomum". It was awarded the 1 st Poster Prize (Poster Session IV) and was published in the abstract form in Rev Port Imunoalergologia 2015; 23(Suppl. 1):64.
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