Suitability of MDA, 8-OHdG and wild-type p53 as genotoxic biomarkers in metal (Co, Ni and Cr) exposed dental technicians: a cross-sectional study

Abstract

Background: High concentrations of Co, Ni, and Cr in the blood serum of dental technicians are strongly associated with free radical formation. It has highly reactive properties that can cause further oxidation of molecule in the vicinity.Purpose: This study intended to investigate whether the Dental Technician occupational exposure of Co, Ni and Cr, could contribute to the high incidence of cancer. Methods: This was a cross-sectional study to dental technicians, performed after acccepting ethical clearance. Blood was sampled in 3 examinations for Co, Ni, Cr using Atomic Absorbance Spectrophotometry (AAS), MDA was examined with TBARS test, also 8 OHdG and wildtype p53 proteins determined by ELISA method. Results: Comparative statistical analysis, showing a significant difference (p < 0.05) between levels of Co, Ni, and Cr in exposed groups to the control group. But, not all variables was proven to be positively correlated, only with Cr, and Co, and negatively correlated with wild-type p53. Conclusion: MDA,8-OHdG and wildtype p53 can be used as genotoxic biomarkers in the metal exposed group, since they can accurately reflect the degree of Oxidative damage.

Telmisartan Mitigates High-Glucose-Induced Injury in Renal Glomerular Endothelial Cells (rGECs) and Albuminuria in Diabetes Mice

“…The production of MDA and 8-OHdG was detected using the protocols according to the instruction previously described …”

“…The production of MDA and 8-OHdG was detected using the protocols according to the instruction previously described. 14 LDH Release. Treated HrGECs were seeded in a 96-well plate at a density of 5 × 10 4 /well, followed by incubation at 37 °C for 4−6 h. Subsequently, the LDH solution was supplemented with lactic acid, 2p-iodophenyl ester-3-p-nitrobenzotetrazole chloride, and lipoamide dehydrogenase, NAD+, and 1.2% saccharose in the PBS buffer was added into the wells.…”

Perbandingan Kadar Kadmium (cd2+) Dalam Darah Antara Pengguna Rokok Elektrik Metode Direct To Lung dan Metode Mouth To Lung Pada Komunitas Vapor Di Kota Bandar Lampung

“…Semakin besar peparan terhadap zat kimia, semakin besar pula risiko keracunan. (Berniyanti et al, 2020) Beberapa logam memiliki fungsi penting dalam tubuh manusia. Zat besi merupakan komponen penting dari hemoglobin dalam sel darah merah.…”

Superoxide Dismutase Due To Occupational Metals Exposure To Nickel On Mutant P53 Dental Technicians

“…5 The base alloy was chosen to replace the gold (IV) alloy type due to its lower cost. 4,6 This content is also found in endodontic instruments, metal brackets, curved cables, ribbons, springs, and cable ties.…”

“…[1][2][3] Since they are biocompatible, have high strength, are corrosive resistant, and are relatively less expensive than gold, cobalt-chromium (Co-Cr), and nickel-chromium (Ni-Cr) alloys are commonly used in dentistry for removable partial dentures, porcelain-fused-to-metal crowns, and metal frames. 4 Metal alloy exposure, as well as improper working conditions and worker protection, is hazardous to worker health during the production process, notwithstanding the bene ts.…”