Background: Nickel levels in dental technicians' blood serum are significantly linked to the generation of free radicals and catalyze the development of reactive oxygen species (ROS) (ROS). An increase in ROS causes oxidative stress and a p53 gene mutation. Superoxide dismutases (SODs) are important antioxidant enzymes that help the body fight oxidative stress. Purpose: The goal of this study is to see if dental technician occupational exposure to Ni contributes to the occurrence of p53 mutations and lower SOD activity in dental technicians. Methods: Blood samples were taken for 3 types of tests, namely Ni examination, with Atomic Absorbance Spectrophotometry (AAS), p53 mutant protein examination by ELISA method, and examination of SOD enzyme activity by Assay Method. Result: The concentration of nickel in dental technicians' blood samples is higher than in controls. Dental technician blood samples have lower levels of SOD than controls. Between dental technicians and controllers, there is a major difference. A negative connection exists between Ni and SOD, a positive correlation exists between Ni and p53mutant, and a negative correlation exists between SOD and p53mutant, according to the Spearman correlation test. Conclusion: A high nickel concentration in the blood serum of a dental worker raises the level of p53 mutants and lowers SOD activity, because p53 mutants have a negative dominant effect on wild p53 types, if wild-type p53 levels decline, p53 mutant levels may rise. Trial Registration: No: 149 / HRECC.FODM / VIII / 2017.
BackgroundNickel levels in dental technicians' blood serum are significantly linked to the generation of free radicals and catalyze the development of reactive oxygen species (ROS) (ROS). An increase in ROS causes oxidative stress and a p53 gene mutation. Superoxide dismutases (SODs) are important antioxidant enzymes that help the body fight oxidative stress.PurposeThe goal of this study is to see if dental technician occupational exposure to Ni contributes to the occurrence of p53 mutations and lower SOD activity in dental technicians.MethodsBlood samples were taken for 3 types of tests, namely Ni examination, with Atomic Absorbance Spectrophotometry (AAS), p53 mutant protein examination by ELISA method, and examination of SOD enzyme activity by Assay Method.ResultThe concentration of nickel in dental technicians' blood samples is higher than in controls. Dental technician blood samples have lower levels of SOD than controls. Between dental technicians and controllers, there is a major difference. A negative connection exists between Ni and SOD, a positive correlation exists between Ni and p53mutant, and a negative correlation exists between SOD and p53mutant, according to the Spearman correlation test.ConclusionA high nickel concentration in the blood serum of a dental worker raises the level of p53 mutants and lowers SOD activity, because p53 mutants have a negative dominant effect on wild p53 types, if wild-type p53 levels decline, p53 mutant levels may rise. Trial Registration: No: 149 / HRECC.FODM / VIII / 2017.
Background: COVID-19 is a global pandemic health problem that is getting a lot of attention around the world today. It is a serious illness that can significantly affect the daily lives of patients and their families in terms of mental health problems, such as post-traumatic stress, depression, anxiety, and insomnia, as well as negatively impacting the quality of patients. Purpose: This study aims to determine the impact of long haulers on the quality of life of COVID-19 survivors in East Java, Indonesia. Methods: Researchers conduct detailed the solution to overcome the problem by using educational videos and sports or routine gymnastics to change the attitude of COVID-19 survivors towards long haul healing is done through the distribution of the two facilities via the google drive link. The drafting team has 2 program plans as a solution to the problems carried out. Results: The prevalence of COVID-19 survivors with long haulers was slightly higher than that of non-long haulers. The results of the knowledge and attitude scores on the pre-test and post-test after viewing the media booklet and educational video showed a significant difference and improvement of quality of life in the result. Conclusion: The level of knowledge, attitudes, and quality of life including the physical health of long haul COVID-19 survivors in East Java experienced a significant increase after being given counseling through online booklets andeducational videos.
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