Suitability of isozyme, RAPD and AFLP markers to assess genetic differences and relatedness among fig (Ficus carica L.) clones

Cabrita, Luís; Aksoy, Uygun; Hepaksoy, Serra; Leitão, José

doi:10.1016/s0304-4238(00)00181-3

Cited by 75 publications

(49 citation statements)

References 15 publications

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“…The common fig (2n = 2x = 26) belongs to the order Urticales, family Moraceae, with over 1400 species classified into about 40 genera (Watson & Dallwitz, 2004) Also, more powerful DNA-based methods have been performed and their efficiency has been proven in the description of the polymorphisms within and between species (Cabrita et al, 2001;Salhi-Hannachi et al, 2006;Ikegami et al, 2009). In the present study, RAPD and SSR markers were used to characterize local fig accessions from the Hatay province of Turkey and to provide a molecular database for fig breeding.…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization of autochthonous Turkish fig accessions

Çalışkan

Polat

Celikkol

et al. 2012

Span. j. agric. res.

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Turkey is one of the main genetic centers for fig tree, Ficus carica L. The genetic variabilities of 76 fig accessions from Hatay province of Turkey were evaluated by analysis of 10 simple sequence repeats (SSR) loci. The number of alleles revealed by SSR analysis ranged from 3 to 12 alleles per locus with a mean value of 6.8. A total of 68 alleles were detected by SSR and the average heterozygosity was higher than the expected one. In addition, seven random amplified polymorphic DNA (RAPD) primers detected a total of 68 clear and reproducible bands, 55 of which were polymorphic, so it was possible to effectively characterize these fig accessions with either marker techniques. In both marker systems, Mantel's correlation between similarity scores and cophenetic values was moderately high (0.90 for RAPD and 0.87 for SSR), which demonstrated that the clustering patterns fitted the data well. The clusters obtained using these types of markers were independent. This study indicated that there is great genetic variability among local fig accessions, making them a valuable genetic source for incorporation into potential breeding programs especially for table fig selections.Additional key words: Ficus carica; genetic diversity; RAPD; SSR. Resumen Caracterización molecular de accesiones autóctonas de higo de TurquíaTurquía es uno de los principales centros genéticos de la higuera, Ficus carica L. Se evaluó, mediante análisis de 10 loci de microsatélites o repeticiones de secuencias simples (SSR), la variabilidad genética de 76 accesiones de higuera muestreadas en la provincia Hatay de Turquía. El número de alelos revelados por análisis SSR varió de 3 a 12 por locus con una media de 6,8; se detectaron en total 68 alelos y la heterocigosidad promedio fue mayor que la heterocigosidad esperada. Además, siete cebadores RAPD detectaron un total de 68 bandas claras y reproducibles, de las cuales 55 fueron polimórficas, por lo que estas accesiones de higuera pudieron ser eficazmente caracterizadas utilizando ambas técnicas. Tanto con SSRs como con RAPDs, la similitud y las correlaciones cofenéticas mediante la prueba de Mantel fueron moderadamente altas (0,90 para RAPD y 0,87 para SSR), lo que demuestra que los patrones de agrupamiento se ajustan bien a los datos. Los grupos obtenidos con este tipo de marcadores fueron independientes. Este estudio encontró una gran variabilidad genética entre accesiones autóctonas de higuera, lo que las hace una fuente valiosa para su incorporación potencial en programas de mejora, especialmente para higos de mesa.

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Section: Introductionmentioning

confidence: 99%

Molecular characterization of autochthonous Turkish fig accessions

Çalışkan

Polat

Celikkol

et al. 2012

Span. j. agric. res.

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“…In addition, DNA markers are not confounded by the environment, pleiotropic, and epistatic effects [13]. In figs, assessment of genetic relatedness and diversity has been investigated by using RFLP, AFLP, SSR, ISSR, and RAPD methods [7,[14][15][16][17][18][19][20][21][22]. Compared with other molecular techniques, RAPD is based on random amplification of bases from short primers.…”

Section: Introductionmentioning

confidence: 99%

Assessments of Biodiversity Based on Molecular Markers and Morphological Traits among West-Bank, Palestine Fig Genotypes (Ficus carica L.)

Basheer-Salimia

Awad²,

Ward³

2012

AJPS

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Both morphological characters and PCR-based RAPD approaches were used to determine the genetic diversity and relatedness among nine fig genotypes grown at the northern region of the West-Bank, Palestine. Although we tested 28 primers for the RAPD technique, only 9 produced reasonable amplification products. A total of 57 DNA loci were detected in which 70.2% were polymorphic. DNA fragments presented a minimum of 3 and a maximum of 9 polymorphic bands using primers OPT-10 and OPA-18, respectively. Primers exhibited collective resolving power values (Rp) of 18.826. The Mwazi genotype showed the highest genetic distances among all of the other genotypes. Morphologically, considerable variations were found using 41 quantitative and qualitative traits. Adloni could be a very promising genotype for fresh consumption due to its very late maturation period, extended harvesting period, variable fruit size, and easy skin peeling. In addition, 7 genotypes presented firm fruits, which are a very important criterion for exporting purposes. Dendrogram constructed by UPGMA based on RAPD banding patterns appear somewhat contradictory to the morphological descriptors particularly with Swadi and Biadi genotypes (closed genetically and distanced morphologically), which might be attributed to the phenotypic modifications caused by environmental differences across regions. These preliminary results will make a fundamental contribution to further genetic improvement of fig crops for the region.

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“…Therefore, caprifigs can be used only as a pollen source for pollination and they don't environmental factors. To overcome this limitation, molecular marker based analysis have been carried out using isoenzyme markers (Cabrita et al, 2001); RAPDs (Khadari et al, 1995;Dalkilic et al, 2011); AFLPs (Baraket et al, 2009); ISSR (Guasmi et al, 2006;Ikegami et al, 2009). Microsattelites or SSRs have become the choice of marker due to their high polymorphism, co-dominance and reproducibility in recent genetic diversity studies for fig cultivars (Khadari et al, 2001;2004;Giraldo et al, 2005;Achtak et al, 2009;Çalışkan et al, 2012;Perez-Jiménez et al, 2012;Ganopoulos et al, 2015;Ferrara et al, 2016) However, genetic characterization of caprifig germplasms using molecular markers such as SSR and RAPD is limited to few studies (Dalkilic et al, 2011;Essid et al, 2015).…”

Section: Introductionmentioning

confidence: 99%