Assessments of Biodiversity Based on Molecular Markers and Morphological Traits among West-Bank, Palestine Fig Genotypes (Ficus carica L.)

Basheer-Salimia, Rezq; Awad, Murad; Ward, Jodie

doi:10.4236/ajps.2012.39150

Cited by 9 publications

(6 citation statements)

References 25 publications

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“…Our results reflected similar findings as reported earlier in studies of the genetic variation in other species at the cultivar level by using RAPD markers (Singh et al, 2012;Kalpanaa et al, 2012;Badfar-Chaleshtori et al, 2012;Basheer-Salimia et al, 2012;Rajesha et al, 2013) and among Palestinian faba bean landraces (Basheer-Salimia et al, 2013).…”

Section: Discussionsupporting

confidence: 92%

Genetic diversity in Tunisian populations of faba bean (Vicia faba L.) based on morphological traits and molecular markers

et al. 2015

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ABSTRACT. Genetic diversity within Vicia faba L. is key to the genetic improvement of this important species. In this study, morphological traits and RAPD molecular markers were used to assess the levels of polymorphism across 12 Tunisian populations, three major and nine minor from different locations. Analysis of morphological traits indicated that the three major populations showed significant differences and the nine minor populations exhibited considerable variation for most traits. The grain yield of the Alia population could be increased by inoculation. Of the seven primers tested, it was clear that the Cs12 primer would be recommend for genetic diversity analysis of V. faba. Within population genetic diversity exhibited 94% of total diversity. Intra-population genetic diversity (H S ) was 0.16, which was clearly higher than between population genetic diversity (D ST = 0.06) UPG-MA showed a high level of genetic variation between major and minor populations of V. faba L. Particularly the minor populations showed a high level of diversity and was divided into two subclusters. Ltaifia was separated from the other populations. In addition to a high grain yield, I.Z. Backouchi et al. 7588©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (3): 7587-7596 (2015) these populations showed the lowest Nei and Shannon indices (H = 0.08 and I = 0.13) justifying their homogeneity. For these reasons, these cultivars can be considered a selected population. However, the Takelsa population showed the highest Nei and Shannon indices (H = 0.13 and I = 0.21), indicating that this population was the most heterogeneous, which is interesting for breeding programs.

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Section: Discussionsupporting

confidence: 92%

Genetic diversity in Tunisian populations of faba bean (Vicia faba L.) based on morphological traits and molecular markers

et al. 2015

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“…The use of Random amplified polymorphic DNA (RAPD) technique offers a simple, fast, efficient and inexpensive method (Basheer-Salimia et al, 2012). Furthermore, it does not need knowledge of marker sequences and can produce abundant polymorphic DNA fragments (Kocsis et al, 2005 andAchtak et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Nature of Gene Action and Efficiency of Molecular Markers for Evaluation of Genetic Polymorphism for Orobanche Tolerance in Faba Bean (Vicia faba L.).

Dora

Rady²,

Abou-Shosha

et al. 2017

Journal of Agricultural Chemistry and Biotechnology

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The present study was carried out to identify the best cross(es) for Orobanche tolerance using genetic variance components and molecular analyses among four parents of faba bean, their six F 1 crosses and six F 2 populations which were produced via half-diallel mating design under Orobanche infected soil condition. Significant mean squares were recorded for parents and crosses for the studied traits. The GCA/SCA ratio was higher than unity for all studied traits, except for number of Orobanche spikes/plant and 100-seed weight. Highly significant positive and negative correlation coefficients were obtained among almost studied traits. Broad sense heritability ranged from intermediate to high and from low to intermediate for narrow sense. Five RAPD and five SSR primers were used for marker-assisted selection for Orobanche tolerance in parents and their F 2 populations. In the case of RAPD primers, three of them showed higher resolving power (RP) rates and were found to be more suitable for genetic diversity analysis in the genotypes under study. In the case of SSR markers, the average value of resolving power was 5.24 per primer. Moreover, primer GA4 was found to be the most suitable for genetic diversity analysis among genotypes under study while, it recorded the highest RP value (7.64). The average values of polymorphic information content were 0.27 and 0.34 per primer based on RAPD and SSR data, respectively. The molecular distance among four faba bean parents and their F 2 bulks ranged from 0.099 to 0.556 by using RAPD and SSR combined data. Positive genotype-specific markers were recorded in some genotypes with molecular sizes ranged from 180 bp to 1584 bp by using both DNA markers. These positive specific markers may play important roles in Orobanche tolerance in faba bean.

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“…The primers BC-261, -322, and -298 were the most useful RAPD primers to assess the genetic diversity since they revealed relatively high Rp rates (5.236, 3.618, and 3.150, respectively). In fact, higher Rp rates also establish the effectiveness of RAPD molecular markers in detecting polymorphism among plant species (Basheer-Salimia et al, 2012).…”

Section: Number/frequency Description 37mentioning

confidence: 99%

“…Compared with other molecular techniques, RAPD is based on random amplification of bases from short primers. The use of the RAPD technique offers a simple, fast, efficient, and inexpensive method (Basheer-Salimia et al, 2012). Furthermore, it does not require prior knowledge of the sequences of the markers and can produce abundant polymorphic fragments (Kocsis et al, 2005;Achtak et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity of Palestine landraces of faba bean (Vicia faba) based on RAPD markers

Basheer-Salimia¹,

Shtaya²,

Awad³

et al. 2013

Genet. Mol. Res.

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ABSTRACT. Until now, neither phenotypic nor molecular approaches have been used to characterize the landraces of Palestine faba beans (Vicia faba). We used PCR-based RAPD markers to determine the genetic diversity and relatedness among 26 Palestinian faba bean landraces (traditional farmers' varieties) from 8 localities in the West Bank, Palestine. In tests with 37 primers, 14 generated no polymorphic bands, 12 exhibited weak and unclear products, and 11 primers produced good amplification products with high intensity and pattern stability. Ninety-four DNA fragments (loci) were detected, with an average of 8.54 loci per primer and size ranging from 160 to 1370 bp. A minimum of 4 and a maximum of 14 DNA fragments were obtained using (OPA-05 and OPA-09) and (BC-261) primers, respectively. The maximum percentage of polymorphic markers was 71.4 (BC-298) and the minimum was 50.0 (OPA-05, -09, -16). The 11 primers exhibited Genetic diversity of faba bean genotypes using RAPD markers relatively high collective resolving power (Rp) values of 26.316, and varied from 0.154 for the OPA-09 primer to 5.236 for the BC-261, with an overall mean of 2.392. The primers BC-261, -322, and -298 were found to be the most useful RAPD primers to assess the genetic diversity of Palestinian faba beans, as they revealed relatively high Rp rates (5.236, 3.618, and 3.150, respectively). Based on the Jaccard coefficient, the genetic distance ranged from 0.358 to 0.069, with a mean of 0.213. We conclude that the RAPD technique is useful for determining genetic diversity and for developing suitable fingerprints for faba bean landraces grown in Palestine.

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Assessments of Biodiversity Based on Molecular Markers and Morphological Traits among West-Bank, Palestine Fig Genotypes (Ficus carica L.)

Cited by 9 publications

References 25 publications

Genetic diversity in Tunisian populations of faba bean (Vicia faba L.) based on morphological traits and molecular markers

Genetic diversity in Tunisian populations of faba bean (Vicia faba L.) based on morphological traits and molecular markers

Nature of Gene Action and Efficiency of Molecular Markers for Evaluation of Genetic Polymorphism for Orobanche Tolerance in Faba Bean (Vicia faba L.).

Genetic diversity of Palestine landraces of faba bean (Vicia faba) based on RAPD markers

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