Nature of Gene Action and Efficiency of Molecular Markers for Evaluation of Genetic Polymorphism for Orobanche Tolerance in Faba Bean (Vicia faba L.).

Abstract

The present study was carried out to identify the best cross(es) for Orobanche tolerance using genetic variance components and molecular analyses among four parents of faba bean, their six F 1 crosses and six F 2 populations which were produced via half-diallel mating design under Orobanche infected soil condition. Significant mean squares were recorded for parents and crosses for the studied traits. The GCA/SCA ratio was higher than unity for all studied traits, except for number of Orobanche spikes/plant and 100-seed weight. Highly significant positive and negative correlation coefficients were obtained among almost studied traits. Broad sense heritability ranged from intermediate to high and from low to intermediate for narrow sense. Five RAPD and five SSR primers were used for marker-assisted selection for Orobanche tolerance in parents and their F 2 populations. In the case of RAPD primers, three of them showed higher resolving power (RP) rates and were found to be more suitable for genetic diversity analysis in the genotypes under study. In the case of SSR markers, the average value of resolving power was 5.24 per primer. Moreover, primer GA4 was found to be the most suitable for genetic diversity analysis among genotypes under study while, it recorded the highest RP value (7.64). The average values of polymorphic information content were 0.27 and 0.34 per primer based on RAPD and SSR data, respectively. The molecular distance among four faba bean parents and their F 2 bulks ranged from 0.099 to 0.556 by using RAPD and SSR combined data. Positive genotype-specific markers were recorded in some genotypes with molecular sizes ranged from 180 bp to 1584 bp by using both DNA markers. These positive specific markers may play important roles in Orobanche tolerance in faba bean.

Evaluation of the genetic variability of faba bean (Vicia faba L.) genotypes using agronomic traits and molecular markers

“…This difference may be due to the environmental variants and the genotypes adapting to dissimilar ecological conditions. These results agree with those obtained by Dora et al (2017) and Abdalla et al (2015). Positive and negative correlations occur through diverse morphological and agronomic traits (Singh et al 2017).…”

Genetic diversity between faba bean hybrids in relation to heterosis using molecular and agronomic data

“…2014and Obiadalla-Ali et al 2015, who reported that the percentage of polymorphic bands detected ranged from 33% to 100% with an average of 66.47% and the average of amplified bands was 5.42 polymorphic bands per primer. Fifty four amplified fragments with sizes ranged from 113bp to 1647bp were obtained with an average of 10.8 loci per primer and the polymorphism percentage ranged from 50% to 100% with an average 81% (Dora et al, 2017) and 86.9% polymorphism obtained by Alghamdi (2009). While; 53.57% polymorphism percent and 140 bands with an average 7.77 bands per primer were obtained by Tahir (2015).…”

“…were obtained by Basheer-Salimia et al 2013revealed that the genetic distance ranged from 0.069 to 0.358, with an average 0.213. And Dora et al (2017) reported genetic distance ranged from 0.099 to 0.556 on the basis of RAPD and SSR combined data among four faba bean genotypes and their seven F 2 bulks. The highest value indicates that the two genotypes are distantly related.…”

“…hybridization of faba bean and onion (Tanttawi et al, 2007;Vajire et al, 2017 andDora et al, 2017). RAPD analysis was assisting faba bean breeders as index to developed genotypes with varied genetic background in order to sustainability in faba production in country (Tahir, 2015).…”

“…Obtaining higher heterotic expression in F 1s and broad spectrum of variability in segregating population, were obtained from increasing the genetic diversity between parents (Shekhawat et al, 2001 andJohnson et al, 2015). Significant variations for agro-morphological traits; plant height, branchs number, pods number, seeds number and seed yield/plant of faba genotypes indicated to significant correlation between agro-morphological traits and results of AFLP technique marker of DNA (Ammar et al, 2015) and also for RAPD technique marker (Dora et al, 2017). Seed characters of faba bean are contributed markedly and significantly from their own genes to the genetic variation (Dieckmann & AMAL M. ABD EL-MAGEED Link, 2010).…”