Sugarcane (Saccharum sp. Hybrid) Propagated in Headspace Renovating Systems Shows Autotrophic Characteristics and Develops Improved Anti-oxidative Response

Abstract

Previous results have shown that sugarcane plantlets micropropagated in Temporary Immersion Bioreactors (TIB) demonstrated a better morphology and physiological behaviour when compared to plantlets propagated in Gelled Medium (GM). The present work focuses on the onset of oxidative stress symptoms at transfer to ex vitro and during acclimatization. The specific ROS being produced were identified and tissue-located by infiltrating leaves with specific O 2 −. and H 2 O 2 staining dyes, respectively NBT and DAB. TIB plantlets showed trichomes stained with NBT and DAB, their density decreasing with time. Stomata were coloured with NBT and DAB in GM and, at the end of acclimatization, plantlets from both systems presented the lowest level of staining of both stomata and trichomes. The response of the anti-oxidative system was also analysed through in vitro and in gel enzyme activities and transcription levels of genes for key response enzymes. At the end of the in vitro phase, GM plantlets showed higher activities of APX and MDHAR, while CAT, GR, GT and DHAR activities increased in TIB. At the end of acclimatization SOD and CAT increased mainly in TIB, while GM induced the increase of APX. The immunobloting of peroxiredoxins showed that Prxs were expressed at higher levels in TIB plantlets, some showing polymerization. The transcription of genes coding for key response enzymes was strongly upregulated in GM plantlets. In conclusion and comparing with GM, TIB produced plantlets closer to autotrophy and with improved mobilization of the anti-oxidative response.

Promoting Utilization of Saccharum spp. Genetic Resources through Genetic Diversity Analysis and Core Collection Construction

“…In recent years, sugarcane has gained increasing attention as a biofuel crop due to its high biomass yield potential [4] . As a C4 plant, sugarcane is one of the world's most efficient crops in converting solar energy into chemical energy through photosynthesis and has a favorable energy input/output ratio [5] , [6] . Besides sucrose-based ethanol production, which replaces 30% of the gasoline consumed in Brazil [7] , sugarcane lignocellulosic biomass-based ethanol is an increasingly attractive biofuel to supplement fossil fuels.…”

Morphological and physiological responses of proliferating shoots of teak to temporary immersion and BA treatments

“…Different procedures have been developed to avoid these problems (Ziv, 2005). Among them, the use of temporary immersion systems (TIS) to improve in vitro growth and plant quality in different species (see review Etienne and Berthouly, 2002;Quiala et al 2006;Roels et al 2006;Aragón et al 2009, Pérez-Alonso et al, 2009Yan et al, 2010, Sankar-Thomas andLieberei, 2011). However, few forestry species, Eucalyptus sp (Castro and González, 2002;McAlister et al 2005), Pinus radiata D. Don (AitkenChristie and Jones, 1987) and Crescentia cujete L. (Murch et al 2004) have been successfully propagated by TIS.…”

In Vitro Propagation of Sugarcane for Certified Seed Production

“…However, to reduce the labor required and increase efficiency, temporary immersion systems (TISs) have been successfully used to improve in vitro sugarcane multiplication [12][13][14][15][16]. The principle of these systems is the immersion of explants for a determined time and frequency. )…”