In Vitro Propagation of Sugarcane for Certified Seed Production

Bello-Bello, Jericó Jabín; Mendoza-Mexicano, Maurilio; Pérez-Sato, Juan Antonio

doi:10.5772/intechopen.74037

Cited by 11 publications

(7 citation statements)

References 19 publications

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“…In this regard, meristem tip culture may be used to remove viruses. Consistent with this proposition, Lal et al (2015) and Bello-Bello et al (2018) have stated that micropropagation (meristem tip culture) enables the renewal of aged and deteriorated varieties as well as the sanitation of diseased varieties.…”

Section: Resultsmentioning

confidence: 82%

Analysis of declining trends in sugarcane yield at Wonji-Shoa Sugar Estate, Central Ethiopia

Dengia¹,

Dechassa

Wogi

et al. 2023

Exp Results

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Yield decline has been the hallmark of Ethiopian sugarcane plantations. However, the extent and causes of the decline have not yet been empirically studied, making it difficult to manage the problem. This study aimed at analyzing the long-term yield data (1954–2022) with respect to variety and soil type. Thus, 8,923 records of yield data were summarized and sorted into decades, varieties, and soil types and then analyzed by applying Mann-Kendall and Tukey’s tests. The fields were classified and mapped using ArcGIS 10.3. The results revealed that 69% of the plantation fields were classified as “yield declining,” and the overall rate of decline has been 8.4 quintals ha−1 year−1 (R2 = 0.76). The rate of decline was higher for older than newer varieties and for vertisols than cambiols. Therefore, the older varieties should be micropropagated or replaced with improved ones, and the vertisols should be amended through practices such as green manuring, improved fallows, etc.

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Section: Resultsmentioning

confidence: 82%

Analysis of declining trends in sugarcane yield at Wonji-Shoa Sugar Estate, Central Ethiopia

Dengia¹,

Dechassa

Wogi

et al. 2023

Exp Results

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“…The plant tissue culture technique allows the establishment, manipulation, and development of cells, tissues, or organs under aseptic conditions [15], while micropropagation is one of the most important global agricultural biotechnology techniques routinely used for the rapid generation of high-quality, uniform, and disease-free plant material [16]. Different in vitro sugarcane micropropagation protocols are available to produce seed canes of high phytosanitary quality (healthy) and with genetic purity (similar to their mother plants) [17].…”

Section: In Vitro Culture Of Apical Meristemsmentioning

confidence: 99%

Biotechnological Strategies Adopted for Sugarcane Disease Management in Tucumán, Argentina

Racedo,

Noguera,

Castagnaro

et al. 2023

Plants

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Sugarcane diseases can be controlled by an integrated management approach where biotechnological tools can successfully contribute. The Obispo Colombres Agroindustrial Experimental Station (EEAOC) in Tucumán (Argentina’s main sugarcane producer) has successfully implemented multiple strategies that greatly enhance the productivity of sugarcane fields. The local breeding program develops resistant varieties by applying molecular markers to reveal the presence of Bru1 gene for brown rust resistance throughout the EEAOC germplasm collection. In addition, SNP alleles linked to novel sources of resistance were identified following a selective genotyping strategy. Another strategy is the implementation of a seed cane sanitation project using hydrothermal therapy, an in vitro culture technique, molecular diagnosis of diseases, and bionanoparticles. As a result, the incidence of systemic diseases has significantly decreased in the production fields. More recently, the use of biological products has shown to be effective for disease control in EEAOC varieties. In summary, several biotechnological strategies including molecular markers associated with resistant sources, in vitro culture of apical meristems, molecular diagnostic techniques, and the use of bioproducts are being successfully used for the sustainable management of sugarcane diseases in Tucumán, Argentina.

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“…The advantages over the traditional techniques are not needing much manpower, large nurseries, logistic storage, transportation, and irrigation [2]. Recently, many commercial plantations have applied micropropagation, especially to produce and propagate their elite sugarcane varieties [3][4][5] Most of the commercially available sugarcanes are complex interspecific hybrids with polyploid genomes, primarily between Saccharum officinarum and Saccharum spontaneum [6]. On one side, polyploidy in sugarcane is considered an advantage for breeding programs as it allows the development of sugarcane varieties with multiple desired traits [7][8][9].…”

Section: Introductionmentioning

confidence: 99%

Callus stage and morphology affect the DNA yield on the DNA extraction from the sugarcane in vitro callus

Aksa,

Setiawati,

Minarsih

et al. 2023

IOP Conf. Ser.: Earth Environ. Sci.

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The main concern with the micropropagation of sugarcane (Saccharum officinarum) with a complex polyploid genome is the frequent occurrence of somaclonal variations. Therefore, genetic stability analysis is often required to ensure the uniformity of the micropropagated sugarcane, as early as in the callus stage. DNA extraction is the initial and important step of the genetic stability analysis. In this experiment, we compared the yield of DNA extraction from the sugarcane embryogenic and non-embryogenic calli. The sugarcane non-embryogenic callus was initiated from immature rolled leaf explants of the 6 months-old plant cane in the callus induction media, that were incubated in the dark room. The embryogenic calli were obtained by routine subcultures of the non-embryogenic calli every six weeks. The results showed that the extraction yield from the non-embryogenic calli was far lower than that from the embryogenic calli, most likely due to the high-water content of the non-embryogenic calli. Furthermore, histological analysis using Safranin and Fast Green staining revealed the low lignin content and nuclei in the non-embryogenic calli cell mass, causing the transparent and watery appearance of the calli and might influence the low DNA extraction yield.

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In Vitro Propagation of Sugarcane for Certified Seed Production

Cited by 11 publications

References 19 publications

Analysis of declining trends in sugarcane yield at Wonji-Shoa Sugar Estate, Central Ethiopia

Analysis of declining trends in sugarcane yield at Wonji-Shoa Sugar Estate, Central Ethiopia

Biotechnological Strategies Adopted for Sugarcane Disease Management in Tucumán, Argentina

Callus stage and morphology affect the DNA yield on the DNA extraction from the sugarcane in vitro callus

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