Sucesso do resfriamento e congelamento de sêmen de pirapitinga Brycon nattereri

Oliveira, Alexmiliano Vogel de; Viveiros, Ana Tereza de Mendonça; Maria, Alexandre Nízio; Freitas, Rilke Tadeu Fonseca de; Izaú, Z. A.

doi:10.1590/s0102-09352007000600025

Cited by 43 publications

(40 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Sperm cells are stained in eosin-nigrosin (Blom 1950) and viewed in a light microscope. This technique can be used to confirm the (2004) B. nattereri 0.25-or 0.5-ml straw Dry-shipper 60°C or 50°C for 8 s Oliveira et al (2007) B. orbignyanus 0.5-ml straw Dry-shipper 37-50°C for 10 s Bedore (1999) 0.5-ml straw Dry-shipper 60°C for 5 s Murgas et al (2001) 0.5-ml straw Dry-shipper 50°C for 10 s Murgas et al (2003) 0.5-ml straw Dry-shipper 60°C for 8 s Maria et al (2006a, b); Viveiros et al (2007) B. orthotaenia 0.5-ml straw Dry-shipper 35°C for 7-10 s Melo and Godinho (2006) L. macrocephalus 0.5-ml straw Dry-shipper 30°C for 6 s Ribeiro and Godinho (2003) L. obtusidens 1-ml tubes 13 cm above LN 2 surface 50- 60°C Coser et al (1987) 0. (Maria et al 2006a).…”

Section: Post-thaw Sperm Evaluationmentioning

confidence: 99%

Sperm quality and cryopreservation of Brazilian freshwater fish species: a review

Viveiros

Godinho

2008

Fish Physiol Biochem

116

View full text Add to dashboard Cite

The Brazilian freshwater fish diversity is the richest in the world. Only 0.7% of all Brazilian species have had any aspect of their sperm biology addressed up to this date. The majority of the fish species described in this review migrate during the spawning season (a phenomenon known as piracema). Urbanization, pollution, hydroelectric dams and deforestation are some of the causes of stock depletion or even local extinction of some of these species. The knowledge concerning sperm quality and minimum sperm:egg ratio is important to maximize the use of males without reducing hatching rates. Furthermore, sperm cryopreservation and gene banking can guarantee the conservation of genetic diversity and development of adequate breeding programs of native fish species. In this review, we present and evaluate the existing information on Brazilian fish species that have been subject to sperm quality and cryopreservation studies. The following parameters were evaluated: volume of extractable sperm, sperm motility, sperm concentration, freezing media, freezing methods, and post-thaw sperm quality. Although the existing protocols yield relatively high post-thaw motility and fertilization rates, the use of cryopreserved sperm in routine hatchery production is still limited in Brazil.

show abstract

Section: Post-thaw Sperm Evaluationmentioning

confidence: 99%

Sperm quality and cryopreservation of Brazilian freshwater fish species: a review

Viveiros

Godinho

2008

Fish Physiol Biochem

116

View full text Add to dashboard Cite

show abstract

“…Only a few migratory Brazilian fish species have already had their semen cryopreserved, for example, Pseudoplatystoma corruscans (Carolsfeld et al 2003); Piaractus mesopotamicus and Leporinus elongates (Carolsfeld et al 2003; Streit Jr, Benites, Moraes, Ribeiro, Sakaguti & Caldieri 2006); Brycon nattereri (Oliveira 2006); and B. orbignyanus (Bedore 1999; Carolsfeld et al 2003; Murgas, Miliorini & Silva 2003; Maria, Viveiros, Freitas & Oliveira 2006).…”

Section: Introductionmentioning

confidence: 99%

A morphological classification proposal for curimba (Prochilodus lineatus) sperm damages after cryopreservation

Miliorini

Murgas

Rosa

et al. 2010

Aquaculture Research

View full text Add to dashboard Cite

The present study evaluated some cryoprotectants and concentrations for curimba (Prochilodus lineatus) semen preservation from a simple methodology of analysis of sperm morphology. Semen of nine males was diluted (1:4) into eight cryosolutions, all of them containing 5% of BTS™ Minitüb, still comprising methanol or dimethyl sulphoxide (DMSO) at four concentrations: 5%, 7.5%, 10% and 12.5%. Morphological sperm analysis was performed on an optical microscope at × 1000 magnification. Damages on the head, midpiece and tail rest (the main and terminal portions) were investigated and classified as primary or secondary, according to the assumed influence on fertilization. There was a linear reduction in the total damages with an increase in the cryoprotectant concentration. Samples with DMSO showed the lowest percentage of fractured tails and tail stumps. The cryopreservation protocol retained the fertilizing potential of the sperm after freezing and both methanol 5% and DMSO 7.5% conferred adequate protection to the curimba sperm cells. Sperm morphology, as pointed out here, must be incorporated into routine fish sperm (cryopreserved or not) analysis once sperm defects influence fertilization directly. To the best of our knowledge, this was the first report on curimba sperm damages after cryopreservation by optical microscopy. The classification model presented in this study can be adequate to tackle fish sperm damages.

show abstract

“…Just as some native species have efficient protocols for freezing semen -Brycon insignis (Viveiros et al, 2011), B. orbignyanus (Maria et al, 2006) and B. nattereri (Oliveira et al, 2007) -the protocol to freeze the semen of C. macropomum was established recently (Maria et al, 2011), but it contains the egg yolk as external cryoprotector, whose substance can bring harm to sperm cells (Tiersch & Green, 2011). The extender solution most used in the protocols is solution of 5% glucose (Ohta & Izawa, 1996;Godinho & Viveiros, 2011), which maintains osmotic pressure (isosmotic) in the sperm cell and thus prevents sperm activation.…”

Section: Introductionmentioning

confidence: 99%

Functional integrity of Colossoma macropomum (Cuvier, 1816) sperm cryopreserved with enriched extender solutions

et al. 2015

View full text Add to dashboard Cite

Cryoprotectant solutions are used to protect the sperm from alterations caused by the low temperature in the cryopreservation process. We evaluated the quality of Colossoma macropomum semen after freezing, using dimethyl sulfoxide (DMSO) as a cryoprotectant, combined with two extender solutions (T1 -Solution 1: Glucose 90.0 g/L, Sodium Citrate 6.0 g/L, EDTA 1.5 g/L, Sodium Bicarbonate 1.5 g/L, Potassium Chloride 0.8 g/L, Gentamycin Sulphate 0.2 g/L, and T2 -Solution 2: Glucose 90.0 g/L, ACP ® -104 10.0 g/L). Motility rate and motility time did not differ between T1 and T2 and were lower than fresh semen. The number of normal sperm was significantly different in treatments T1 (15.1%) and T2 (21.9%), and both showed a reduction in the percentage of normal sperm compared to fresh semen (57.4%). The values found for the rates of fertilization and hatching, mitochondrial functionality and sperm DNA, did not differ between the treatments (T1 and T2). Regarding membrane integrity, there was a higher percentage of spermatozoa with intact membranes in T1 (53.4%) than T2 (43.7%). The extender solutions, combined with 10% DMSO, maintained the sperm DNA intact in almost all the C. macropomum sperm cells, however there was a loss in their functionality.As soluções crioprotetoras são utilizadas para proteger os espermatozoides das alterações causadas por baixas temperaturas durante o processo de criopreservação. Avaliamos a qualidade do sêmen de Colossoma macropomum após o congelamento, utilizando dimetilsulfóxido (DMSO) como crioprotetor, combinado com duas soluções diluidoras (T1 -Solução 1: Glicose 90,0 g/L, Citrato de Sódio 6,0 g/L, EDTA 1,5 g/L, Bicarbonato de Sódio 1,5 g/L, Cloreto de Potássio 0,8 g/L, Sulfato de Gentamicina 0,2 g/L, e T2 -Solução 2: Glicose 90,0 g/L, ACP®-104 10,0 g/L). A taxa de motilidade (%) e o tempo de motilidade (s) não diferiram entre T1 e T2, porém foram mais baixos do que no sêmen fresco. O número de espermatozoides normais foi significativamente diferente nos tratamentos T1 (15,1%) e T2 (21,9%), e ambos mostraram uma redução na porcentagem de espermatozoides normais, comparado ao sêmen fresco (57,4%). Os valores encontrados para as taxas de fertilização e eclosão, funcionalidade mitocondrial e DNA do esperma, não diferiram entre os tratamentos (T1 e T2). Para a integridade da membrana, houve uma porcentagem mais elevada de espermatozóides com a membrana intacta em T1 (53,4%) do que T2 (43,7%). As soluções diluentes combinadas com DMSO a 10% preservaram o DNA espermático intacto em quase todas as células do sêmen de C. macropomum, mas houve perda na funcionalidade dos mesmos.

show abstract

Sucesso do resfriamento e congelamento de sêmen de pirapitinga Brycon nattereri

Cited by 43 publications

References 7 publications

Sperm quality and cryopreservation of Brazilian freshwater fish species: a review

Sperm quality and cryopreservation of Brazilian freshwater fish species: a review

A morphological classification proposal for curimba (Prochilodus lineatus) sperm damages after cryopreservation

Functional integrity of Colossoma macropomum (Cuvier, 1816) sperm cryopreserved with enriched extender solutions

Contact Info

Product

Resources

About