Succinic acid biosynthesis from cane molasses under low pH by Actinobacillus succinogenes immobilized in luffa sponge matrices

Comprehensive investigation of succinic acid production by Actinobacillus succinogenes: a promising native succinic acid producer

“…To realize industrial production and make the bioprocess more economically feasible, the adoption of low-cost feedstocks for succinic acid production offers a promising alternative. Various substrates have been used for succinate production by using A. succinogenes, including sake lees, 45 cane molasses, 46 corn stover, 38 corn fiber, 47 wheat flour, 48 corncob hydrolysates, 49 corn stalk, cotton stalk, 50 and sugarcane bagasse hemicellulose hydrolysate. 51 Among these, waste byproducts, such as glycerol from biodiesel production and waste lignocellulose biomass, including corn stover and corncob, are most promising substrates.…”

“…Then the luffa sponge matrices system was further introduced for cell immobilization to enhance succinic productivity, resulting in 49.6% enhanced productivity compared with repeated batch culture. 46 Simultaneous saccharification and fermentation of corn stover was carried out for succinic acid production by A. succinogenes CGMCC 1593 in a 5 L stirred bioreactor. Results showed that succinic acid yield could be significantly increased from corn stover pretreated with diluted alkali Table 2.…”

From second generation feed-stocks to innovative fermentation and downstream techniques for succinic acid production

“…Reported yields for the former 237 are around 40 wt% (Borges & Pereira, 2011) up to 80 wt% (Chen, Tao, & Zheng, 2016) when multiple enzymatic pretreatment is applied. While for sugar cane molasses SA yields are between nearly 70 wt% (Cao et al, 2018b) to 80 wt% (Liu et al, 2008;Shen et al, 2015), depending on the pretreatment steps and nitrogen sources. For both sugarcane bagasse and molasses A. succinogenes was used as the microbial host.…”

Improved dark fermentation of cane molasses in mesophilic and thermophilic anaerobic fluidized bed reactors by selecting operational conditions

“…3 Thus, molasses is a cheap carbohydrate that can be used as a resource on the fermentative generation of butyrate, 3 butanol, 4 hydrogen, [5][6][7][8][9] and succinate. 10 Succinic acid is generated as an intermediate metabolite in the tricarboxylic metabolite cycle. 11 This chemical has important applications in different industries and is one of the most interesting chemical products of the biorefinery processes, especially because the majority of succinic acid commercialized is produced from fossil sources.…”

“…11 This chemical has important applications in different industries and is one of the most interesting chemical products of the biorefinery processes, especially because the majority of succinic acid commercialized is produced from fossil sources. 10,11 However, some literature studies reported succinic acid production from sugarcane molasses using pure cultures of microorganisms such as Actinobacillus succinogenes 10,12 and Escherichia coli. 13 Although the use of pure cultures results in higher yields, the need for sterilization might make the dark fermentation unfeasible for practical application because of the higher costs associated with a more complex process.…”