Comprehensive investigation of succinic acid production by Actinobacillus succinogenes: a promising native succinic acid producer

Abstract

The biological production of succinic acid has attracted a great deal of attention due to the depletion of fossil fuels and concerns regarding environmental issues. As a natural producer of succinic acid, Actinobacillus succinogenes is one of the most promising candidates owning to its high production capability, wide carbon utilization spectrum, and robustness to environmental factors. This review details current achievements attempting to improve the efficiency of succinic acid production, not only in terms of raw materials and metabolic engineering strategies but also in terms of process optimization, such as work on neutralizing agents, redox potential, and the supply of CO2. Strategies to reduce the accumulation of byproduct accumulation and to improve succinic acid synthesis are discussed. © 2019 Society of Chemical Industry and John Wiley & Sons, Ltd.

A Genomic Perspective on the Potential of Wild-Type Rumen Bacterium Enterobacter sp. LU1 as an Industrial Platform for Bio-Based Succinate Production

“…A distinctive capacity to utilize a broad spectrum of carbon sources, including monosaccharides, both pentoses and hexoses, disaccharides, and polyhydroxy alcohols, is one of the most significant features for industrial producers of bulk chemicals [2]. Biochemical tests based on the API 50CHE system revealed that the Enterobacter sp.…”

“…LU1 is a Gram-negative, rod-shaped, wild-type bacterium that has been isolated from goat rumen by bacterial enrichment and selective culture for succinic acid (SA)-producing bacteria [1]. SA, with a molecular formula of C 4 H 6 O 4 , is natural organic acid that exists in animals, plants, and microorganisms [2]. It has been recognized as one of the top 10 most promising C4-chemical building blocks with prospects for bio-based commercial production [3,4].…”

“…However, depletion of fossil fuels and our ever-increasing concern about environmental pollution urge us to establish sustainable processes for bio-based production of high-valuable commodity and specialty chemicals from waste feedstock. Compared with petrochemical synthesis, biotechnological production of SA is characterized by high efficiency, low cost, and renewability of substrates [2]. Therefore, a sustainable, ecofriendly process for microbial production of SA from renewable feedstock has become a focal point of global interest [9][10][11][12].…”

“…Actinobacillus succinogenes and Anaerobiospirillum succiniciproducens are considered to be promising SA biocatalysts for industrial application due to their high production efficiency [2]. Basfia succiniciproducens and Mannheimia succiniciproducens are other microorganisms that have been recognized as native succinate producers [13,14].…”

Production of succinic acid from pineapple peel waste

“…The biotechnological production of succinic acid has been explored extensively using various biomass and agro-residues as precursors (e.g., sake lees, cane molasses, corn stover, corncob, bagasse, cassava root) (Yang et al 2019). However, only few attempts have been made using pineapple waste as the substrate for succinic acid production.…”