Successful in vitro fertilization and embryo transfer in cynomolgus monkeys

In Vitro Manipulation of Nonhuman Primate Gametes for Embryo Production and Embryo Transfer.

“…Among the several types of media reported, my research group selected TYH medium [103], which was developed for IVF of mouse eggs. Furthermore, by incubation of the spermatozoa in medium that contained caffeine and dibutyryl cyclic AMP, sperm capacitation and hyperactivated motion were induced in spermatozoa of the rhesus monkey (Macaca mulatta) [11], the pig-tailed monkey (Macaca nemestrina) [18], and the cynomolgus monkey [5,56]. Our study also confirmed this finding in cynomolgus monkeys [84], Japanese monkeys [83], and African green monkeys (Cercopithecus aethiops) [78].…”

“…Establishing an efficient ET technique is therefore an important task in this research field. In 1984, 6 years after the first successful ET in man [94], ET in nonhuman primates was successful in the rhesus monkey [7], the baboon [17] and the cynomolgus monkey [5]. My research group also used ET to the oviduct of cynomolgus monkeys and successfully generated offspring (unpublished data) (Fig.…”

Genome Banking for Vertebrates Wildlife Conservation

“…These authors noted that 1.5M Me 2 SO was superior to 1.0M glycerol; the latter causing severe osmotic damage. Relying on success in IVF followed by embryo transfer (Balmaceda et al, 1984), pregnancies resulting from frozen-thawed IVF-produced embryos in cynomolgus monkeys (Macaca fascicularis) were reported (Balmaceda et al, 1986). Fifty-six cynomolgus macaque embryos were cryopreserved at the four-to eight-cell stage using 1.5 M Me 2 SO as cryoprotectant and the slow-freezing technique.…”

In vitro fertilization‐embryo transfer in nonhuman primates: The technique and its applications

“…Abbasi and associates (1987) noted that 50% of the ovulations occurred between 24-48 hours after hCG injection in hMG-treated macaques, another 37% occurred by 72 hours, with the remaining 10% ovulating in 4-5 days. Investigators have routinely aspirated follicles for oocyte collection between 24 (Lopata et al, 1988) and 38 hours (Balmaceda et al, 1984) after hCG injection.…”

“…Potential problems can be overcome by transferring fresh embryos into surrogate mothers during a spontaneous cycle (Balmaceda et al, 1984;Bavister et al, 1984) or cryopreserved-and-thawed embryos into natural or surrogate mothers during a subsequent normal cycle . If these options are unavailable, monitoring and possible hormonal supplementation of the luteal phase should be considered.…”