In vitro fertilization‐embryo transfer in nonhuman primates: The technique and its applications

Wolf, Don P.; Thomson, James A.; Zelinski, Mary B.; Stouffer, Richard L.

doi:10.1002/mrd.1080270313

Cited by 105 publications

(56 citation statements)

References 143 publications

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“…Oocytes were examined for the presence of cytoplasmic vesicles and evidence of nuclear maturation. Only oocytes that had extruded a first polar body were considered appropriate for fertilization [4,9]. In this preliminary study, ova were used for a variety of pilot experiments.…”

Section: Discussionmentioning

confidence: 99%

“…Fourteen, 21, 16 and 20 oocytes (n = 71) were collected from 4 animals (average = 17.75/treatment cycle). 91% of the oocytes collected (n = 64) were morphologically normal, all in metaphase 2 and of grade I and II, which are considered appropriate for fertilization [4,9]. 18 out of the 64 oocytes were used in a pilot ICSI experiment and a 38.8% fertilization rate was achieved (7/18).…”

Section: Egg Retrievalmentioning

confidence: 99%

“…PMSG or human /menotropin, urofollitropin/) for assisted reproduction [2]. Follicular aspiration in NHP has been performed by laparotomy or laparoscopy [1,[7][8][9][10]. Limited data are available on the controlled ovarian stimulation (COS) with a GnRH agonist (GnRHa) and highly purified human FSH (hphFSH) and noninvasive ultrasound-guided follicular aspiration in NHP [6][7][8][9].…”

Section: Introductionmentioning

confidence: 99%

“…Follicular aspiration in NHP has been performed by laparotomy or laparoscopy [1,[7][8][9][10]. Limited data are available on the controlled ovarian stimulation (COS) with a GnRH agonist (GnRHa) and highly purified human FSH (hphFSH) and noninvasive ultrasound-guided follicular aspiration in NHP [6][7][8][9]. The data collected will help scientists consider strategies for improving controlled ovarian stimulation protocols for assisted reproduction technology and for animal breeding, conservation and gamete banking.…”

Section: Introductionmentioning

confidence: 99%

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Controlled ovarian stimulation and ultrasound guided follicular aspiration in the baboon (Papio cynocephalus anubis)

et al. 2001

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-The objective of this study was to investigate whether baboon females respond to an ovarian stimulation protocol incorporating pituitary suppression with a GnRH agonist (GnRHa) and highly purified human FSH (hphFSH) with follicular development and oocyte maturation. An ovulation induction protocol was applied to 5 adult female baboons with a history of regular menstrual cycles (33-34 days). A long-acting GnRHa implant containing goserelin acetate was placed s.c. on days 22-24 of their menstrual cycle. Daily hphFSH (75 IU im) treatments were started ~10 days following menses. When the majority of the follicles were ³ 5 mm in diameter and the E2 levels had reached a maximum, hCG (2000 IU i.m.) was administered to induce final maturation of the oocytes and ovulation. 30 to 34 h after hCG administration, transabdominal follicular aspiration was performed using a variable frequency transvaginal transducer with ultrasound. A total of 71 oocytes were collected (average: 17). 91% of the oocytes were morphologically normal indicating that they were appropriate for in vitro insemination. ovarian stimulation / highly purified human FSH / GnRH agonist / ultrasound-guided oocyte retrieval / baboon Résumé -Stimulation ovarienne contrôlée et aspiration folliculaire guidée par ultrason chez le babouin (Papio cynocephalus anubis). L'objectif de cette étude a été d'examiner si une femelle babouin répond au protocole d'une stimulation ovarienne incorporant une suppression hypophysaire grâce à un agoniste de GnRH (GnRHa) et une FSH humaine hautement purifiée (hphFSH) avec développement folliculaire et maturation des oocytes. Un procédé d'induction d'ovulation a été appliqué à 5 babouins femelles adultes ayant des cycles menstruels réguliers (33-34 jours). Un implant de GnRHa à action prolongée contenant de l'acétate de gosereline a été placé en sous cutané les jours 22-24 de leurs cycles menstruels. Les traitements quotidiens de l'hphFSH (75 UI i.m.) ont Reprod. Nutr. Dev. 41 (2001) [531][532][533][534] 531

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Section: Discussionmentioning

confidence: 99%

Section: Egg Retrievalmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Controlled ovarian stimulation and ultrasound guided follicular aspiration in the baboon (Papio cynocephalus anubis)

et al. 2001

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“…An IVF technique has been developed and successfully used for several types of nonhuman primates [20,21,24,27,38,40,42,72,80,112,114,115,117]. For example, we first reported IVF with frozen-thawed spermatozoa from cynomolgus monkeys, in which the embryo obtained developed to the hatched blastocyst stage [84] (Fig.…”

Section: In Vitro Fertilization (Ivf)mentioning

confidence: 99%

In Vitro Manipulation of Nonhuman Primate Gametes for Embryo Production and Embryo Transfer.

Sankai

2000

Exp. Anim.

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Abstract:Since nonhuman primates are closely related to humans and share many physical similarities, they are important for use in research areas such as human infectious diseases, reproduction, physiology, endocrinology, metabolism, neurology and longevity. To develop and maintain these animals, we must establish techniques for in vitro manipulation of spermatozoa and eggs. For a decade my research group has been conducting basic research to establish embryo manipulation techniques and to clarify the reproductive phenomena in nonhuman primates. This article summarizes the past research on in vitro manipulation of nonhuman primate gametes, from collection of reproductive cells and in vitro fertilization to the birth of offspring after embryo transfer, as well as the current status of these research areas. The studies summarized here will directly lead to the development of standard techniques for practical and comprehensive use in nonhuman primates.

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In vitro fertilization and preimplantation embryo development of African green monkeys (Cercopithecus aethiops)

1997

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Ovaries of five adult female African green monkeys were stimulated by repeated administrations of equine chorionic gonadotrophin (eCG), followed by a single administration of human chorionic gonadotrophin (hCG). Oocytes were collected from enlarged follicles 28 h after hCG administration and incubated in vitro for 288 h. Oocytes that had extruded the first polar body were inseminated with spermatozoa that had been incubated for 4 to 6 h in medium with caffeine and dibutyryl cyclic AMP. Of these oocytes, 66% were fertilized and the incidence of polyspermy was 37%. Eighty‐two percent of the fertilized eggs cleaved, with some developing into expanded blastocysts. Am. J. Primatol. 43:43–50, 1997. © 1997 Wiley‐Liss, Inc.

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In vitro fertilization‐embryo transfer in nonhuman primates: The technique and its applications

Cited by 105 publications

References 143 publications

Controlled ovarian stimulation and ultrasound guided follicular aspiration in the baboon (Papio cynocephalus anubis)

Controlled ovarian stimulation and ultrasound guided follicular aspiration in the baboon (Papio cynocephalus anubis)

In Vitro Manipulation of Nonhuman Primate Gametes for Embryo Production and Embryo Transfer.

In vitro fertilization and preimplantation embryo development of African green monkeys (Cercopithecus aethiops)

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