In Vitro Manipulation of Nonhuman Primate Gametes for Embryo Production and Embryo Transfer.

Abstract

Abstract:Since nonhuman primates are closely related to humans and share many physical similarities, they are important for use in research areas such as human infectious diseases, reproduction, physiology, endocrinology, metabolism, neurology and longevity. To develop and maintain these animals, we must establish techniques for in vitro manipulation of spermatozoa and eggs. For a decade my research group has been conducting basic research to establish embryo manipulation techniques and to clarify the reproductive phenomena in nonhuman primates. This article summarizes the past research on in vitro manipulation of nonhuman primate gametes, from collection of reproductive cells and in vitro fertilization to the birth of offspring after embryo transfer, as well as the current status of these research areas. The studies summarized here will directly lead to the development of standard techniques for practical and comprehensive use in nonhuman primates.

Characterization of an immortalized oviduct cell line from the cynomolgus monkey (Macaca fascicularis)

“…In cynomolgus monkeys (the main subjects of our research), embryos can develop to the blastocyst stage in culture [23], and obtaining a baby after embryo transfer is also possible [22]. But, as in humans, cynomolgus monkey embryos are difficult to develop in vitro , so some improvement of culture medium is required.…”