Subverting bacterial resistance using high dose, low solubility antibiotics in fibrin

Woolverton, Christopher J.; Huebert, K.; Burkhart, Blanche; MacPhee, Martin

doi:10.1007/bf02565167

Cited by 16 publications

(10 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The antibiotic streptomycin has low aqueous solubility, but when encapsulated by fibrin matrices had enhanced efficacy in preventing host lethality in a rat model of experimental multidrug-resistant S. aureus peritonitis. 70 However, the contribution of fibrinogen to host defense against S. aureus peritonitis goes beyond simple clumping or entrapment of bacteria.…”

Section: Fibrinogen As a Central Mediator Of Host Defense In S Aureumentioning

confidence: 99%

Fibrinogen Is at the Interface of Host Defense and Pathogen Virulence in Staphylococcus aureus Infection

Ko¹,

Flick

2016

Semin Thromb Hemost

View full text Add to dashboard Cite

Fibrinogen not only plays a pivotal role in hemostasis but also serves key roles in antimicrobial host defense. As a rapidly assembled provisional matrix protein, fibrin(ogen) can function as an early line of host protection by limiting bacterial growth, suppressing dissemination of microbes to distant sites, and mediating host bacterial killing. Fibrinogen-mediated host antimicrobial activity occurs predominantly through two general mechanisms, namely, fibrin matrices functioning as a protective barrier and fibrin(ogen) directly or indirectly driving host protective immune function. The potential of fibrin to limit bacterial infection and disease has been countered by numerous bacterial species evolving and maintaining virulence factors that engage hemostatic system components within vertebrate hosts. Bacterial factors have been isolated that simply bind fibrinogen or fibrin, promote fibrin polymer formation, or promote fibrin dissolution. Staphylococcus aureus is an opportunistic gram-positive bacterium, the causative agent of a wide range of human infectious diseases, and a prime example of a pathogen exquisitely sensitive to host fibrinogen. Indeed, current data suggest fibrinogen serves as a context-dependent determinant of host defense or pathogen virulence in Staphylococcus infection whose ultimate contribution is dictated by the expression of S. aureus virulence factors, the path of infection, and the tissue microenvironment.

show abstract

Section: Fibrinogen As a Central Mediator Of Host Defense In S Aureumentioning

confidence: 99%

Fibrinogen Is at the Interface of Host Defense and Pathogen Virulence in Staphylococcus aureus Infection

Ko¹,

Flick

2016

Semin Thromb Hemost

View full text Add to dashboard Cite

show abstract

“…When poorly soluble antibiotics are entrapped in a fibrin matrix at concentrations greater than their solubility, they are deposited as hetero geneously sized crystals and released into the medium gradually [42]. In a more recent study, it has been shown that the release of entrapped molecules is also hampered by their binding to the matrix proteins or those entrapped within the matrix during the formation of fibrin net works [5,43].…”

Section: Discussionmentioning

confidence: 99%

Beaded Plasma Clot: a Potent sustained-release, drug-delivery System

Ahmad

Fatima

Owais³

et al. 2011

Therapeutic Delivery

View full text Add to dashboard Cite

Aim: The aim of the study was to prepare a drug-entrapped, beaded form of blood plasma for possible sustained drug delivery. Method: Blood plasma mixed with various drugs was enriched with CaCl2 and transferred in the form of small droplets on to a glass slide covered with parafilm. Clot formation was induced by incubation at 37°C. Results: Plasma-bead entrapped tetracycline, amphotericin B and daunorubicin were released gradually in vitro. Crosslinking of the beads with glutaraldehyde decreased the release rate of drugs remarkably. The plasma bead-entrapped cefotaxime administered subcutaneously in mice was released in a slow and sustained fashion and remained in circulation for a longer duration than the antibiotic administered in the free form. Conclusion: The plasma beads have potential for the sustained delivery of drugs in vivo, since their preparation does not require additional thrombin or other proteins and can be readily accomplished by using autologous plasma, thereby minimizing the risk of immunological complications.

show abstract

“…Many papers demonstrated that FG combined with antibiotics inhibits bacterial growth in vitro and in vivo (Harry et al ., 1991; Woolverton et al ., 1999). Here, we aimed to investigate the possible role of FG as the conditioned media for bacterial growth.…”

Section: Discussionmentioning

confidence: 99%

Bacteriological study of autologous cryoprecipitate‐derived fibrin glue as the operative sealant

Kinoshita

Udagawa

Tsutsumi

et al. 2005

Transfusion Medicine

View full text Add to dashboard Cite

Fibrin glue (FG) is frequently used to seal and cover the anastomoses in many operations such as cardiovascular surgery or orthopaedic surgery. However, in case of gastrointestinal surgery, anastomoses are potentially contaminated, and FG may promote bacterial growth, increasing the risk of leakage. The purpose of this study was to examine the effect of cryoprecipitate-derived FG (CryoFG) on bacterial growth. Bacterial growth on the CryoFG and on the commercial FG (Beriplast P) was evaluated and compared with that on control medium. In addition, the complement activities were evaluated by heat inactivation or addition of guinea-pig complement to the experimental settings. The CryoFG inhibited the growth of Escherichia coli, whereas the commercial FG had no effect. Heat inactivation of the CryoFG inhibited the bactericidal effect of CryoFG. Addition of guinea-pig complement to the heat-inactivated CryoFG could almost restore the bactericidal activity, suggesting the important role of complement. This study showed that the CryoFG preserved the complement activity, which inhibited the in vitro growth of E. coli. Therefore, we concluded that the application of the CryoFG for gastrointestinal surgical anastomoses not only would be safe but also has the advantage of reducing bacterial infection.

show abstract

Subverting bacterial resistance using high dose, low solubility antibiotics in fibrin

Cited by 16 publications

References 22 publications

Fibrinogen Is at the Interface of Host Defense and Pathogen Virulence in Staphylococcus aureus Infection

Fibrinogen Is at the Interface of Host Defense and Pathogen Virulence in Staphylococcus aureus Infection

Beaded Plasma Clot: a Potent sustained-release, drug-delivery System

Bacteriological study of autologous cryoprecipitate‐derived fibrin glue as the operative sealant

Contact Info

Product

Resources

About