2011
DOI: 10.4155/tde.11.30
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Beaded Plasma Clot: a Potent sustained-release, drug-delivery System

Abstract: Aim: The aim of the study was to prepare a drug-entrapped, beaded form of blood plasma for possible sustained drug delivery. Method: Blood plasma mixed with various drugs was enriched with CaCl2 and transferred in the form of small droplets on to a glass slide covered with parafilm. Clot formation was induced by incubation at 37°C. Results: Plasma-bead entrapped tetracycline, amphotericin B and daunorubicin were released gradually in vitro. Crosslinking of the beads with glutaraldehyde decreased the release ra… Show more

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Cited by 13 publications
(11 citation statements)
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“…The PB preprations were leaky for daunorubicin and tetracycline but release of amphotericin B was remarkably slow, apparently because of the binding of the antifungal to the bead proteins [104,105]. Release rates of PBentrapped drugs could be markedly lowered by treating the PBs with glutaraldehyde [36]. The in vivo release of PB-entrapped cefotaxime injected subcutaneously in the Swiss mice was slow and Page 13 of 51 A c c e p t e d M a n u s c r i p t 13 sustained; cefotaxime administered in the fibrin formulation was detectable in circulation even after 144 h, while in those receiving the free drug, no drug was detectable beyond 108 h. The remarkable advantages of the PBs and PMPs include the possibility of using autologous plasma, non-requirement of fibrinogen purification, as well as extraneous thrombin or transglutaminase.…”
Section: Protection Against Proteolysismentioning
confidence: 96%
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“…The PB preprations were leaky for daunorubicin and tetracycline but release of amphotericin B was remarkably slow, apparently because of the binding of the antifungal to the bead proteins [104,105]. Release rates of PBentrapped drugs could be markedly lowered by treating the PBs with glutaraldehyde [36]. The in vivo release of PB-entrapped cefotaxime injected subcutaneously in the Swiss mice was slow and Page 13 of 51 A c c e p t e d M a n u s c r i p t 13 sustained; cefotaxime administered in the fibrin formulation was detectable in circulation even after 144 h, while in those receiving the free drug, no drug was detectable beyond 108 h. The remarkable advantages of the PBs and PMPs include the possibility of using autologous plasma, non-requirement of fibrinogen purification, as well as extraneous thrombin or transglutaminase.…”
Section: Protection Against Proteolysismentioning
confidence: 96%
“…These include Fibrin Microbeads (FMB) [29], Fibrin Films (FF) [30], Fibrin Discs (FD) [31], Fibrin Threads (FT) [32], Fibrin Microparticles (FMP) [33] Fibrin Nanoparticles (FNP) [34] and Fibrin Nano Constructs (FNC) [35]. More recently, whole blood plasma, induced to clot with help of endogenous thrombin and transglutaminase and formulated as plasma beads (PB) or plasma microparticles (PMPs), has also been studied for its therapeutic delivery potential [36,37]. Pharmaceutical-entrapped fibrin matrices may also be created in situ by injecting mixtures of fibrinogen, the pharmaceutical and thrombin [38].…”
Section: Components Of the Fibrin Matricesmentioning
confidence: 99%
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“…We have reported a procedure for the preparation of plasma beads (PB) from autologous plasma, necessitating the addition of no exogenous enzymes/proteins, with remarkable potential for sustained drug/antigen delivery [ 22 , 23 , 24 ]. In this paper, we report the use of plasma-alginate composite matrices, fibrinolytic inhibitors (aprotinin and 6-amino caproic acid) as well as dual delivery via various liposomal formulations to achieve superior sustained release of entrapped molecules in PB.…”
Section: Introductionmentioning
confidence: 99%