Fibrin matrices: The versatile therapeutic delivery systems

Ahmad, Ejaj; Fatima, Mahvish; Hoque, Mehboob; Owais, Mohammad; Saleemuddin, M.

doi:10.1016/j.ijbiomac.2015.07.054

Cited by 46 publications

(28 citation statements)

References 174 publications

(155 reference statements)

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“…The FDA approved its commercial use as a hemostat in 1998 22) . It has since been used as a topical hemostat for sealing the dura mater incision line, or filling dead space in spinal surgery 2,16,23,25,28) . In case of venous sinus injury during craniotomy, many neurosurgeons use this item to achieve bleeding control as well as to physically defend the sinuses 27,32) .…”

Section: Discussionmentioning

confidence: 99%

“…This natural polymer can maintain its continuous antibacterial activity until it is degraded over time for several weeks. However, it can be influenced by structural limitations of fibrin glue or a saturated state of affinity to vancomycin 2) .…”

Section: Discussionmentioning

confidence: 99%

“…Since it is naturally present in the human body, it has several advantages over other sealants due to low inflammatory response, lack of immune interference, and natural absorption. Recent studies indicate that fibrin sealant acts as a biodegradable drug delivery agent as well as in hemostasis 2,25,28,31) . Morishima et al 23) and Ozaki et al 25) reported that vancomycin-impregnated fibrin sealant effectively prevented prosthetic graft infection.…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of Cumulative and Conditional Antibiotic Release from Vancomycin-Embedded Fibrin Sealant and Its Antibacterial Activity : An In Vitro Study

Shin

Sohn

Cho

et al. 2020

J Korean Neurosurg Soc

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Objective : Fibrin sealants have been used for hemostasis, sealant for cerebrospinal fluid leakage, and adhesive barrier in neurosurgery. Further, as its clinical use and role of an effective drug delivery vehicle have been proposed. This study was performed to measure antibacterial activity and continuous local antibiotic release from different concentrations of vancomycin-impregnated fibrin sealant in vitro. Methods : Antibacterial activity was investigated by disk diffusion test by measuring the diameter of the growth inhibition zone of bacteria (methicillin-resistant Staphylococcus aureus, ATCC29213) from vancomycin-embedded fibrin sealant disc diluted at five different concentrations (C1-C5; 8.33, 4.167, 0.83, 0.083, and 0.0083 mg/disc, respectively). Continuous and conditioned release of vancomycin concentration (for 2 weeks and for 5 days, respectively) were also measured using high-performance liquid chromatography (HPLC) method. To mimic the physiologic wound conditions with in vitro, conditioned vancomycin release in phosphate buffer solution (PBS) was measured and replaced PBS for five consecutive days, half a day or completely daily. Results : In the disk diffusion test, the mean diameters of bacterial inhibition zone were 2.54±0.07 cm, 2.61±0.12 cm, and 2.13±0.15 cm (C1, C2, and C3 respectively) but 1.67±0.06 cm and 1.23±0.15 cm in C4 and C5, respectively. Continuous elution test elicited the peak release of vancomycin from the fibrin sealant at 48 hours, with continued release until 2 weeks. However, conditioned vancomycin release decreased to half or more on day 2, however, the sustainable release was measured over the therapeutic dose (10-20 µg/mL) for 5 days and 4 days in assays of half and total exchange of PBS. Conclusion : This study suggests that fibrin sealant can provide an efficient vehicle for antibiotic drug release in a wide range of neurosurgical procedures and the safe and effective therapeutic dose will be at the concentration embedded of 4.167 mg/disc or more of vancomycin.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Evaluation of Cumulative and Conditional Antibiotic Release from Vancomycin-Embedded Fibrin Sealant and Its Antibacterial Activity : An In Vitro Study

Shin

Sohn

Cho

et al. 2020

J Korean Neurosurg Soc

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“…[78][79][80][81] Gentamicin undergoes controlled release from CS/fucoidan NPs, which renders the NPs into a multiple-function drug delivery system with antibacterial and antioxidant activities that can be used to treat pneumonia. 82 In addition, CS-coated alginate NPs increase the ability of daptomycin to permeate the limbal epithelium of the eye and also increase its ocular accumulation and effect duration.…”

Section: Np-based Antibiotic Delivery Systemsmentioning

confidence: 99%

The antimicrobial activity of nanoparticles: present situation and prospects for the future

Wang¹,

Hu²,

Shao³

2017

IJN

2,715

1,792

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Nanoparticles (NPs) are increasingly used to target bacteria as an alternative to antibiotics. Nanotechnology may be particularly advantageous in treating bacterial infections. Examples include the utilization of NPs in antibacterial coatings for implantable devices and medicinal materials to prevent infection and promote wound healing, in antibiotic delivery systems to treat disease, in bacterial detection systems to generate microbial diagnostics, and in antibacterial vaccines to control bacterial infections. The antibacterial mechanisms of NPs are poorly understood, but the currently accepted mechanisms include oxidative stress induction, metal ion release, and non-oxidative mechanisms. The multiple simultaneous mechanisms of action against microbes would require multiple simultaneous gene mutations in the same bacterial cell for antibacterial resistance to develop; therefore, it is difficult for bacterial cells to become resistant to NPs. In this review, we discuss the antibacterial mechanisms of NPs against bacteria and the factors that are involved. The limitations of current research are also discussed.

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“…Clinical studies have shown that TISSEEL fibrin glue is biocompatible and biostable in the brain of human patients 18 . Importantly, these properties have led to the use of fibrin scaffolds for tissue engineering of adipose, skin, liver, and bone tissues 19 , delivery of therapeutic molecule for various diseases 19,20 , and brachytherapy treatment of brain tumors 21–23 .…”

Section: Introductionmentioning

confidence: 99%

Fibrin matrices enhance the transplant and efficacy of cytotoxic stem cell therapy for post-surgical cancer

et al. 2016

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Tumor-homing cytotoxic stem cell (SC) therapy is a promising new approach for treating the incurable brain cancer glioblastoma (GBM). However, problems of retaining cytotoxic SCs within the post-surgical GBM resection cavity are likely to significantly limit the clinical utility of this strategy. Here, we describe a new fibrin-based transplant approach capable of increasing cytotoxic SC retention and persistence within the resection cavity, yet remaining permissive to tumoritropic migration. This fibrin-based transplant can effectively treat both solid and post-surgical human GBM in mice. Using our murine model of image-guided model of GBM resection, we discovered that suspending human mesenchymal stem cells (hMSCS) in a fibrin matrix increased initial retention in the surgical resection cavity 2-fold and prolonged persistence in the cavity 3-fold compared to conventional delivery strategies. Time-lapse motion analysis revealed that cytotoxic hMSCs in the fibrin matrix remain tumoritropic, rapidly migrating from the fibrin matrix to co-localize with cultured human GBM cells. We encapsulated hMSCs releasing the cytotoxic agent TRAIL (hMSC-sTR) in fibrin, and found hMSC-sTR/fibrin therapy reduced the viability of multiple 3-D human GBM spheroids and regressed established human GBM xenografts 3-fold in 11 days. Mimicking clinical therapy of surgically resected GBM, intra-cavity seeding of therapeutic hMSC-sTR encapsulated in fibrin reduced post-surgical GBM volumes 6-fold, increased time to recurrence 4-fold, and prolonged median survival from 15 to 36 days compared to control-treated animals. Fibrin-based SC therapy could represent a clinically compatible, viable treatment to suppress recurrence of post-surgical GBM and other lethal cancer types.

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Fibrin matrices: The versatile therapeutic delivery systems

Cited by 46 publications

References 174 publications

Evaluation of Cumulative and Conditional Antibiotic Release from Vancomycin-Embedded Fibrin Sealant and Its Antibacterial Activity : An In Vitro Study

Evaluation of Cumulative and Conditional Antibiotic Release from Vancomycin-Embedded Fibrin Sealant and Its Antibacterial Activity : An In Vitro Study

The antimicrobial activity of nanoparticles: present situation and prospects for the future

Fibrin matrices enhance the transplant and efficacy of cytotoxic stem cell therapy for post-surgical cancer

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