Subtypes of bovine adenovirus type 2 exhibit major differences in region E3

Subcloning and Restriction Enzyme Mapping of Bovine Adenovirus Type 2

“…During this study, four EcoKl fragments and two BamHl fragments were observed as was similarly reported by Benko et al [5] and Belâk et al [3,4], The positions of these frag ments on the linear map were identical to that reported by Belâk et al [4]. The average sum of the restriction fragments generated in this study was 32.5 kbp (table 1).…”

“…The average sum of the restriction fragments generated in this study was 32.5 kbp (table 1). This is 0.1 kbp smaller than that reported by Benko et al [5], 0.5 kbp larger than that reported by Belâk et al [4], and 2. Î ----------------------- al.…”

“…Subgroup I, representing serotypes 1, 2, 3 and 9, possesses a common complement-fixing antigen and therefore cross-reacts with other mastadenoviruses. Subgroup-II serotypes (4,5,6, 7 and 8) lack this common antigen and do not cross-react with any other mastadenovirus.…”

“…Its genome consists of approximately 30.3 to 34.8 kbp in a linear double-stranded DNA molecule [3][4][5] and appears to cause pneumonia as well as pneumonic-like symptoms in cattle [6], Although, generally, most adenoviruses have a narrow host range, there have been several reported cases in which BAV2 strains, similar to the prototype strain (No. 19), have been isolated from sheep [7,8].…”

“…19) [4] consisting of only two restric tion enzyme maps for £coRI and BamYU. In this paper, we report on the molecular sub cloning of the internal £a>RI and Sal\ BAV2 (strain No.…”