Transcription mapping of mouse adenovirus type 1 early region 3

Beard, Clayton W.; Ball, Amy Oberhauser; Wooley, E.; Spindler, Katherine R.

doi:10.1016/0042-6822(90)90188-w

Cited by 35 publications

(21 citation statements)

References 48 publications

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“…However, the ORF for putative E3 glycoprotein (designated as E3 ORFA in MAdV-1 genome annotation AC_000012) of the novel virus lacked the correct start and stop codons. This seems to be in agreement with findings of Beard et al (5) indicating that only part of this ORF is an exon in MAdV-1 (start and stop codons are therefore irrelevant) belonging to the E3 class 3 unique region. Never- theless, this finding for the novel virus is only based on sequence analysis and needs confirmation by transcription mapping and cDNA sequencing.…”

Section: Virus Isolation and Identification By Pan-pcr Liver Tissue supporting

confidence: 82%

A Novel Cardiotropic Murine Adenovirus Representing a Distinct Species of Mastadenoviruses

Klempa

Krüger

Auste

et al. 2009

J Virol

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During cell culture isolation experiments to recover Dobrava hantavirus from a suspension of liver from a striped field mouse (Apodemus agrarius), an unknown virus was coisolated. Atypically for hantaviruses, it had extensive cytopathic effects. Using a random PCR approach, it was identified as a novel murine adenovirus, MAdV-3 (for MAdV type 3). A plaque-purified virus clone was prepared and further characterized. The complete genome sequence of MAdV-3 was determined to be 30,570 bp in length. Sequence comparisons to other adenovirus species revealed highest similarity to MAdV-1, the representative of the murine adenovirus A species. However, substantial differences were found in the E1, E3, and E4 genomic regions. The phylogenetic distance of MAdV-3 amino acid sequences for pVIII, protease, polymerase, and hexon from MAdV-1 is markedly higher than 0.1 exchange per position, and, based on our cross-neutralization experiments, MAdV-3 and MAdV-1 can be regarded as different serotypes. Therefore, we propose to classify MAdV-3 as the first isolate of a novel adenovirus species, designated murine adenovirus C (MAdV-C). The novel MAdV-3 virus is not only genetically and serologically distinct from MAdV-1 but also shows a unique organ tropism in infected mice. In contrast to MAdV-1, the virus was not detectable in brain but predominantly infected heart tissue. Thus, infection of mice with cardiotropic MAdV-3 might be an interesting animal model of adenovirus-induced myocarditis.

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Section: Virus Isolation and Identification By Pan-pcr Liver Tissue supporting

confidence: 82%

A Novel Cardiotropic Murine Adenovirus Representing a Distinct Species of Mastadenoviruses

Klempa

Krüger

Auste

et al. 2009

J Virol

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“…Several adenoviruses, originating from different animal species, show DNA homology with HAd-2 [8][9][10][11][12]. In the case of MAd-l, DNA regions recognized in HAd-2 are located in genes coding for structural components [1,[10][11][12][13][14]. The fact that a MAd-2 probe recognizes about the same sequences as MAd-1 in HAd-2 DNA suggests the synthesis of similar hexons and structural proteins by the two murine adenoviruses.…”

Section: Discussionmentioning

confidence: 99%

Genetic relationship between mouse adenovirus-2 (strain K87) and human adenovirus-2

Jacques

1994

FEMS Microbiology Letters

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The DNA of mouse adenovirus strain K87 (MAd-2) was cloned and mapped with restriction endonucleases BglII, ClaI, EcoRI, HindIII and SphI. Large differences were found between the MAd-2 and MAd-1 (strain FL) DNA molecules in terms of number and location of restriction sites. The MAd-2 genome also appeared as larger in size than the MAd-1 genome (34.72 kb vs. 30.14 kb). Our results confirm the existence of two distinct adenovirus species in the mouse. Hybridization experiments, on the other hand, indicate that both MAd-1 and MAd-2 are genetically related to human adenovirus type 2 (HAd-2). Overlapping regions of DNA homology are located in genes coding for HAd-2 structural components which could explain serological relationships observed between the human and the murine adenoviruses.

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“…9). The DNA regions recognized by the MAd-1 probe in HAd-2 contain genes coding for structural components of the virus (Ball et al, 1988(Ball et al, , 1989Beard et al, 1990;Gingeras et al, 1982;Larsen et al, 1979;Raviprakash et at., 1989). The observation that a MAd-2 probe recognizes approximately the same sequences as the MAd-1 probe in HAd-2 DNA suggests that the two MAds have genes encoding similar hexon and structural proteins.…”

Section: Discussionmentioning

confidence: 99%

Molecular cloning, physical mapping and cross-hybridization of the murine adenovirus type 1 and type 2 genomes

Jacques

Cousineau

D'Amours

et al. 1994

Journal of General Virology

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Murine adenovirus (MAd) type 1 strain FL and type 2 strain K87 genomes were cloned into plasmid pAT153 as HindIII restriction fragments. The MAd-1 and MAd-2 DNA genomes, 30"10kb and 34.71 kb in length respectively, were mapped using BgIII, ClaI, EcoRI, HindIII and SphI restriction endonuclease cleavage sites. In view of the large differences found between the MAd-1 and MAd-2 genomes in terms of the number and location of restriction sites, cross-hybridization experiments were performed. Homologous DNA sequences were located on the MAd-1 and MAd-2 physical maps. Both viruses are also genetically related to human adenovirus type 2 (HAd-2). Nucleotide sequences shared by HAd-2 and the MAds code for structural proteins, which may explain the antigenic similarities between these viruses from different origins. Our results confirm the existence of two distinct adenovirus species in the mouse.

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Transcription mapping of mouse adenovirus type 1 early region 3

Cited by 35 publications

References 48 publications

A Novel Cardiotropic Murine Adenovirus Representing a Distinct Species of Mastadenoviruses

A Novel Cardiotropic Murine Adenovirus Representing a Distinct Species of Mastadenoviruses

Genetic relationship between mouse adenovirus-2 (strain K87) and human adenovirus-2

Molecular cloning, physical mapping and cross-hybridization of the murine adenovirus type 1 and type 2 genomes

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