Subcloning and Restriction Enzyme Mapping of Bovine Adenovirus Type 2

Salmon, Kirsty; Esford, Lesley E.; Haj-Ahmad, Yousef

doi:10.1159/000150324

Cited by 9 publications

(6 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Bovine adenoviruses type 2 (BAV2) and bovine adenovi ruses type 3 (BAV3) in subgroup 1 are among the best characterized BAVs. In general, their genomic organiza tion is similar to those of other Ads [2,3] This article is also accessible online at: ww\v. karger.com http://BioMcdNet.com/karger host range, however, BAV2 and BAV3 differ from each other.…”

Section: Introductionmentioning

confidence: 90%

Sequence Analysis of the Terminal Protein Precursor Coding Regions from Bovine Adenovirus Serotypes 2 and 3

et al. 1997

View full text Add to dashboard Cite

As part of our efforts to develop bovine adenoviruses into a vector system, we sequenced the region predicted to encode the terminal protein precursor of either bovine adenoviruses type 2 and bovine adenoviruses type 3. We examined the regions containing the terminal protein precursors and showed that the bovine adenovirus early region 2B had an identical organization to all adenoviruses so far examined. The bovine adenovirus terminal protein precursors and those of other adenoviruses were also compared in a sequence alignment analysis and several highly conserved structural domains were identified. Finally, we showed how the various terminal protein precursors were related in a sequence pair similarity analysis and concluded that the terminal protein precursors of bovine adenoviruses types 2 and 3 are significantly different from each other at the protein sequence level.

show abstract

Section: Introductionmentioning

confidence: 90%

Sequence Analysis of the Terminal Protein Precursor Coding Regions from Bovine Adenovirus Serotypes 2 and 3

et al. 1997

View full text Add to dashboard Cite

show abstract

“…We have previously reported the construction of a library of plasmids containing restriction fragments of the BAV2 strain 19 [8] and BAV3 strain WBR-1 [7] viral genomes cloned in pUC19. For BAV2, three plasmids containing 0-17.5% (pBB12), 5.1-19.3% (pPE30) and 19.3-30.6% (pEB113) of the viral genome were used for sequencing.…”

Section: Plasmidsmentioning

confidence: 99%

“…Our previous analysis of the BAV2 and BAV3 genomes revealed that their genetic organizations are similar to other Ads belonging to the Mastadenovirus subfamily [7][8][9][10]12]. Since all known sequences for Ad pol indicate that the gene is located between 10 and 24% of the genome, we targeted similar regions in the DNA of BAV2 and BAV3 for sequencing analysis.…”

Section: Identification Of Orfs For the Dna Polmentioning

confidence: 99%

“…We have previously reported the preliminary structural characterization of the genomes of these BAV serotypes with respect to their cloning and restriction enzyme analysis [7,8]. Sequencing analyses of the BAV3 E1 and pIX regions [9], BAV2 E1 region [10], BAV3 E3 [11], and BAV2 E3 [12] have provided evidence that the genomes of BAVs have similar genetic organizations to those of the well-characterized human Ads.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Sequencing Analysis of the Region Encoding the DNA Polymerase of Bovine Adenovirus Serotypes 2 and 3

et al. 1998

View full text Add to dashboard Cite

Bovine adenoviruses (BAVs) are important pathogens causing significant economic losses to the cattle industry. We have been interested in the differences among serotypes of these viruses, particularly in their pathogenicity and host range. As part of our efforts to better understand these viruses, we have determined the nucleotide sequences for serotype 3 (BAV3) at map coordinates beween 11.7 and 23.7% and for serotype 2 (BAV2) between 13.1 and 24.0%. Analyses of these sequences revealed large open reading frames (ORFs) encoded within the leftward-reading strand of the viral DNA. The coding capacity of the ORF in BAV3 is 1,167 amino acid residues and 1,138 in BAV2. A search in the GenEMBL protein sequence databank for homology to the predicted polypeptide products of these ORFs established their identity as that for the adenovirus (Ad) DNA polymerase (DNA pol). The deduced polypeptide sequences were aligned with each other and with other known Ad DNA pols to reveal regions of homology and similarity. The comparison at the amino acid sequence level not only showed that the bovine Ad DNA pols from the two serotypes are quite distinct from each other, but also revealed that Ad DNA pols contain multiple domains that are highly conserved among human, canine and bovine Ads. These conserved domains are likely important for the multiple functions attributed to Ad DNA pol, which include catalysis of its own initiation complex, elongation of nascent DNA strand, as well as correction of DNA replication errors.

show abstract

“…The size of the genome reported varies from 30.3 to 34.8 kb [9][10][11][12] and restriction maps of BAV2 (No. 19) for a num ber of enzymes are available [ 10,12], The main difference …”

mentioning

confidence: 99%

Sequence Analysis of the Putative E3 Region of Bovine Adenovirus Type 2

Esford

Haj-Ahmad²

1994

Intervirology

Self Cite

View full text Add to dashboard Cite

Adenoviruses are nonenveloped icosahedral-shaped particles. The double-stranded viral DNA genome contains four major early transcription units, designated El (a and b), E2 (a and b), E3 and E4, which are expressed in a regulated manner soon after infection. The gene products of the region E3, shown to be nonessential for viral replication in vitro, are believed to be involved in counteracting host immunosurveillance. Human adenovirus type 5 DNA sequences of transcription units L4 and L5 adjacent to E3 were used to localize E3 within the bovine adenovirus type 2. The DNA sequences between 74.8 and 84.4 mu containing E3 and the fiber gene were determined. The E3 region was found to consist of about 2.3 kb pairs and to encode four proteins longer than 60 amino acids. However, these four open reading frames did not show significant homology to any other known adenovirus DNA or protein sequence.

show abstract

Subcloning and Restriction Enzyme Mapping of Bovine Adenovirus Type 2

Cited by 9 publications

References 4 publications

Sequence Analysis of the Terminal Protein Precursor Coding Regions from Bovine Adenovirus Serotypes 2 and 3

Sequence Analysis of the Terminal Protein Precursor Coding Regions from Bovine Adenovirus Serotypes 2 and 3

Sequencing Analysis of the Region Encoding the DNA Polymerase of Bovine Adenovirus Serotypes 2 and 3

Sequence Analysis of the Putative E3 Region of Bovine Adenovirus Type 2

Contact Info

Product

Resources

About