Substantiation of medical use of antimicrobial remedies, containing decamethoxin®

Paliy, V. G.; Назарчук, Олександр; Paliy, D. V.; Iakovets, K. I.

doi:10.24061/2413-0737.xxi.1.81.2017.21

Cited by 5 publications

(1 citation statement)

References 1 publication

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Субстанція декаметоксину (ДКМ ® ) являє собою речовину з групи четвертинних амонієвих сполук. За даними літературних джерел антимікробний ефект ДКМ® обумовлений механізмом, що поєднує ушкодження клітинної мембрани бактерій та лізис їх протопластів, а також змінює проникність клітинної оболонки мікробної клітини, викликаючи її деструкцію [12]. ДКМ® демонструє активність щодо грампозитивних і грамнегативних бактерій (стафілококи, стрептококи, дифтерійна і кишкова палички, сальмонели, протей, клебсієли, шигели, псевдомонади, клостридії), деяких грибів (дріжджеподібні гриби, окремі види пліснявих грибів) [13,14].…”

Section: обговорення результатів дослідженняunclassified

Impact of a Gel Composition Containing Various Substances on Indices of Immunity and Tissue Inflammation in Palatine Tonsils of Patients With Catarrhal Gingivitis During Chronic Tonsillitis

Bezhuk,

Martovlos (Hodovana)

2024

Act. Probl. of the Modern Med.

View full text Add to dashboard Cite

The study of pharmacotherapeutic (immunomodulating) action in conditions of in vitro cell cultures is currently considered appropriate and justified for determining the action of drugs at the preclinical level. It is also important to address the pathogenetic nature in using such agents, especially fro local application. This study investigated the efficacy of a multicomponent gel containing decamethoxine and hyaluronic acid in treating chronic catarrhal gingivitis among patients with chronic tonsillitis. The purpose of the work is to assess the efficacy of a gel containing decamethoxine and hyaluronic acid on the tissue of the palatine tonsils in the therapy of chronic catarrhal gingivitis in patients with chronic tonsillitis. Materials and methods. To investigate the effects of the main active components in the gel, we followed the recommendations of I.P. Kaidashev et al. for testing pharmacological preparations in in vitro cultures. Tissue samples from the palatine tonsils were obtained from 20 patients with chronic tonsillitis and chronic catarrhal gingivitis undergoing tonsillectomy. All procedures adhered to the guidelines set by the Laboratory of Pathophysiology and Immunology at Prof. O.S. Kolomiichenko Institute of Otolaryngology, National Academy of Sciences of Ukraine. Palatine tonsil tissue was incubated in 199 medium (Serva, Germany) containing gentamicin sulfate (Darnytsa, Ukraine) at a concentration of 120 μg/ml for 20 minutes at 4-8°C. After that, the hemorrhagic parts were removed from the whole tonsil and placed into Eagle's medium (Sigma, USA) and rinsed three to four times. Subsequently, the prepared tissue was placed in sterile vials containing enriched Eagle's medium supplemented with L-glutamine, sodium bicarbonate, 100x vitamin concentrate, 5% fetal calf serum, and 60 μg/mL gentamicin. Tonsil tissue was dissected using scissors and then washed repeatedly (5-7 times) with fresh medium to remove loosely attached cells, then, the resulting cell suspension was filtered. All procedures were conducted within a sterile laminar flow cabinet. Cell viability was assessed using light microscopy (Olympus-23, Japan) and a hemocytometer. A final cell suspension containing 2.5 million lymphocytes per milliliter of medium was prepared. Suspensions with a viability of less than 90% (blue, "dead" cells) were excluded from further experiments. Results. Treatment of in vitro palatine tonsil cells from patients with chronic catarrhal gingivitis and recurrent tonsillitis with the main components of the developed gel (decamethoxine and hyaluronic acid) did not induce significant changes in the concentration of α and γ-interferons. Our findings suggest that the gel composition reduces the levels of pro-inflammatory factors: interleukin-1β and immune complexes. Additionally, it appears to stimulate the production of the antimicrobial factor antistreptolysin-O by tonsil cells, potentially leading to increased antibody production against hemolytic streptococcus antigens. Conclusion. Based on the in vitro findings, the investigated gel composition containing decamethoxine and hyaluronic acid demonstrates potential anti-inflammatory and antimicrobial properties that stimulate the production of the antimicrobial factor antistreptolysin-O by tonsil cells, which might lead to increased antibody production against hemolytic streptococcus antigens.

show abstract

Section: обговорення результатів дослідженняunclassified

Impact of a Gel Composition Containing Various Substances on Indices of Immunity and Tissue Inflammation in Palatine Tonsils of Patients With Catarrhal Gingivitis During Chronic Tonsillitis

Bezhuk,

Martovlos (Hodovana)

2024

Act. Probl. of the Modern Med.

View full text Add to dashboard Cite

show abstract

Characteristics of antioxidant and NO-synthase systems of blood lymphocytes under the action of decamethoxine

2020

Eksp.klin.fiziol.biohim.

View full text Add to dashboard Cite

Introduction. Antiseptics are widely used in the system of antimicrobial measures for the prophylaxis and treatment of infectious diseases [1-4]. Antiseptic drugs have mostly the microbicidal effect on the skin surface, mucous membranes and surfaces of a wound and body cavities [1-4]. They are dissolved in water and lipids. One of the most widespread antiseptics is the drug 'Decamethoxine'. Other drugs such as horosten, decasan, auridexan, septephril were created on its basis [2,3]. Decamethoxine is a chemical compound [1, 10-decamethylene bis (N, N-dimethylmethoxycarbonylmethyl) ammonium dichloride] that has a wide range of the antimicrobial action on Gram-positive (staphylococcus, streptococcus, pneumococcus), Gram-negative (gonococcus, meningococcus) cocci, diphtheria corynebacteria, enterobacteria, pseudomonads, protozoa, dermatophytes, yeasts-like fungi of the genus Candida, chlamydia and viruses. When applied, the formation of variants of microorganisms resistant to decamethoxine occurs very slowly. Since decamethoxine is a surfactant, it changes the permeability of membranes of microbial cells which leads to the degradation and destruction of microorganisms and this determines its bactericidal action [5]. However, the antiseptic action of this substance is based not only on the bactericidal, but also on bacteriostatic activity. Pathological processes that occur in the cells of the body when inflammatory processes and pathological conditions are caused primarily by the violation of regulatory systems, the activation of lipid peroxidation processes and the violation of the antioxidant protective system [6-10]. Under the influence of extreme factors of different origin on organisms, the formation of reactive oxygen species (ROS) in living organisms increases. Under normal physiological conditions, ROS is not accumulated in cells. Any stress response of the organism is usually accompanied by a short-term increase in the number of ROS. ROS activates the expression of redox-sensitive genes in cells, many of which are required to protect cells from the toxic effects of oxidative stress, particularly glutathione peroxidase, catalase, superoxide dismutase, glutathione reductase, γ-glutamylcysteine synthetase. A number of antioxidant systems plays a major role in the neutralization of secondary peroxidation products and other oxidizing materials, namely, glutathione antiperoxide system as the most powerful one [8, 10]. Due to the catalytic activity of glutathione peroxidase (GP) in cells, the reduction of H 2 O 2 and hydroperoxides of organic molecules to corresponding hydroxy compounds occurs. This process is performed by using reduced glutathione.

show abstract

Effectiveness of Using Domestic Quaternary Ammonium Antiseptic in General Medicine and Dentistry (Modern View and Clinical Case)

Bezhuk

Hodovana

2023

Proc Shevchenko Sci Soc Med Sci

View full text Add to dashboard Cite

Decamethoxine (DCM®) is a substance from a group of quaternary ammonium compounds. According to literary sources, the antimicrobial effect is due to a mechanism that combines damage to the cell membrane of bacteria and lysis of their protoplasts, as well as changes the permeability of the microbial cell membrane, causing its destruction. DCM® demonstrates activity against gram-positive and gram-negative bacteria (staphylococci, streptococci, diphtheria and Escherichia coli, salmonella, proteus, klebsiella, shigella, pseudomonads, clostridia), some fungi (yeast fungi, mold fungi). There are also reports of the antiviral activity of this compound. The purpose of this study was to highlight the results of the current work of Ukrainian scientists and analyze the properties and broad aspects of DCM® antiseptic, as well as demonstrate their clinical case for its effectiveness in periodontal practice. The analysis of the results of various publications allowed us to evaluate the full range of DCM® properties in terms of its therapeutic potential in modern preclinical and clinical studies, particularly in periodontal practice.

show abstract

Substantiation of medical use of antimicrobial remedies, containing decamethoxin®

Cited by 5 publications

References 1 publication

Impact of a Gel Composition Containing Various Substances on Indices of Immunity and Tissue Inflammation in Palatine Tonsils of Patients With Catarrhal Gingivitis During Chronic Tonsillitis

Impact of a Gel Composition Containing Various Substances on Indices of Immunity and Tissue Inflammation in Palatine Tonsils of Patients With Catarrhal Gingivitis During Chronic Tonsillitis

Characteristics of antioxidant and NO-synthase systems of blood lymphocytes under the action of decamethoxine

Effectiveness of Using Domestic Quaternary Ammonium Antiseptic in General Medicine and Dentistry (Modern View and Clinical Case)

Contact Info

Product

Resources

About