Subcritical carbon dioxide foaming of polycaprolactone for bone tissue regeneration

Duarte, Rui M.; Correia‐Pinto, Jorge; Reis, Rui L.; Duarte, Ana Rita C.

doi:10.1016/j.supflu.2018.05.019

Cited by 21 publications

(14 citation statements)

References 58 publications

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“…In a study by Duarte et al, Obtained scaffolds of PCL, β-TCP and dexamethasone using CO 2 as a foaming agent, obtaining supernatants for only 24 hours for MTT testing. They found that there is a decrease in cell viability from ~130% for PCL to ~122% for PCL/β-TCP and ~99% for PCL/β-TCP/5% dexamethasone, concluding that, although cell viability changes, surfaces do not affect the metabolic activity of L929 osteoblast cells 49 .…”

Section: Discussionmentioning

confidence: 99%

Functional evaluation and testing of a newly developed Teleost’s Fish Otolith derived biocomposite coating for healthcare

Montañez

Carreño

Escobar

et al. 2020

Sci Rep

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Polymers such as polycaprolactone (PCL) possess biodegradability, biocompatibility and affinitywith other organic media that makes them suitable for biomedical applications. in this work, a novel biocomposite coating was synthesised by mixing pcL with layers of calcium phosphate (hydroxyapatite, brushite and monetite) from a biomineral called otolith extracted from Teleost fish (Plagioscion Squamosissimus) and multiwalled carbon nanotubes in different concentrations (0.5, 1.0 and 1.5 g/L). The biocomposite coating was deposited on an osteosynthesis material Ti6Al4V by spin coating and various tests such as fourier transformation infrared spectroscopy (ftiR), Raman spectroscopy, scanning electron microscopy (SeM), transmission electron microscopy (teM), scratch tests, Mtt reduction cytotoxicity, HoS cell bioactivity (human osteosarcoma) by alkaline phosphatase (ALP) and fluorescence microscopy were performed to comprehensively evaluate the newly developed biocoating. it was found that an increase in the concentration of carbon nanotube induced microstructural phase changes of calcium phosphate (cp) leading to the formation of brushite, monetite and hydroxyapatite. While we discovered that an increase in the concentration of carbon nanotube generally improves the adhesion of the coating with the substrate, a certain threshold exists such that the best deposition surfaces were obtained as PCL/CP/CNT 0.0 g/L and PCL/CP/CNT 0.5 g/L.Metallic biomaterials used in implantology are usually inert, since there is no ion active exchange. This property allows the cells to recognize the microenvironment, proliferate and differentiate in a better way. For this reason, biocompatible materials in coatings such as calcium ceramics with a chemical nature like bone, or natural or synthetic polymers allows easy incorporation of bioactive materials or active ingredients making the recovery of hard tissue faster.One of the widely used polymers in tissue engineering is polycaprolactone (PCL -poly ε-caprolactone). It possesses semi-crystalline state, hydrophobic character, good solubility, low melting point, adequate degradation rate and excellent compatibility 1-3 , making it a good candidate for use as a biocoating material. One drawback as with many other polymers that the PCL has is its low mechanical strength which is proposed to be modified in this paper via addition of functionalised multiwalled carbon nanotubes (MWCNT) (Fig. 1). Recently, it has been shown that PCL and carbon nanotubes can forms an interface that improves certain properties in the composite 4 . Besides, carbon nanotubes can be functionalized, allowing other molecules to be adhered to the walls and ends, improving the properties of the materials. This strategy has been widely used to incorporate calcium phosphates (CP) like hydroxyapatite 5-7 , CaCO 3 2 or polymers for applications such as cancer treatment, drug transport, biotechnological applications and energy [8][9][10] . As Surmenev et al. indicate, much of the in vitro results of inorganic Ca-P coatings show...

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Section: Discussionmentioning

confidence: 99%

Functional evaluation and testing of a newly developed Teleost’s Fish Otolith derived biocomposite coating for healthcare

Montañez

Carreño

Escobar

et al. 2020

Sci Rep

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“…Typical functionalization of PCL for bone applications include reinforcement with a mineral component such as β‐TCP (Abbah, Lam, Hutmacher, Goh, & Wong, 2009; Abbah, Lam, Ramruttun, Goh, & Wong, 2011b), or CaP with or without further addition with BMP‐2 (Abbah, Lam, Ramruttun, Goh, & Wong, 2011a; Yong et al, 2014), yet herein, the combination of pDA and pMAA provided the adhesive properties to PCL, while simultaneously inducing mineralization of the structure (Figure 6). In a previous work, the authors have successfully incorporated dexamethasone and TCP via carbon dioxide foaming of PCL, which could be added to the formulation to further improve osteogenesis (R. M. Duarte et al, 2018). This ability to induce the formation of a bone‐like HA layer has been reported to be a factor that strongly enhances the integration of the scaffold with the bone tissue and therefore pivotal for a successful healing and tissue regeneration (El‐Ghannam & Ducheyne, 2011; Hill, 2011).…”

Section: Discussionmentioning

confidence: 99%

“…In previous work (R. M. Duarte, Correia‐Pinto, Reis, & Duarte, 2018), the authors reported successful carbon dioxide foaming of PCL in subcritical, physiologically friendly conditions (5.0 MPa and 45°C), while incorporating bioactive agents such as dexamethasone and β‐tricalcium phosphate (TCP), to aid osteogenesis. The use of CO 2 as a porogen agent has significant advantages over others, namely, the fact that this is a physical foaming agent.…”

Section: Introductionmentioning

confidence: 99%

Advancing spinal fusion: Interbody stabilization by in situ foaming of a chemically modified polycaprolactone

Duarte

Correia‐Pinto

Reis

et al. 2020

J Tissue Eng Regen Med

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Spinal fusion (SF) surgery relies on medical hardware such as screws, cages and rods, complemented by bone graft or substitute, to stabilize the interventioned spine and achieve adequate bone ingrowth. SF is technically demanding, lengthy and expensive. Advances in material science and processing technologies, proposed herein, allowed the development of an adhesive polymeric foam with the potential to dismiss the need for invasive hardware in SF. Herein, 3D foams of polycaprolactone doped with polydopamine and polymethacrylic acid (PCL pDA pMAA) were created. For immediate bone stabilization, in situ hardening of the foam is required; therefore, a portable high-pressure device was developed to allow CO 2 foaming within bone defects. Foams were characterized by scanning electron microscopy, Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy. Adhesive properties of PCL pDA pMAA outperformed PCL when tested using glass surfaces (p < 0.001) or spinal plugs (p < 0.05). No cytotoxicity was observed, and bioactivity was confirmed by the CaP layer formed upon 7 days immersion in simulated body fluid. As proof of concept, PCL pDA pMAA was extruded in-between ex vivo porcine vertebrae, and micro-computed tomography revealed similar properties to those of trabecular bone. This novel system presents great promise for instrumentation-free interbody fusion.

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“…Recently, the environmentally friendly packaging material designed especially for drugs and food is one of the most significant commercial applications of PCL. However, the commercially available PCL with a linear chain structure has insufficient heat resistance, low melt strength, and deficient thermomechanical strength, greatly restricting its processability such as thermoforming, foaming as well as blow molding . For that reason, many research studies have been performed on the modification of its physicochemical structure and properties by blending, copolymerization and grafting …”

Section: Introductionmentioning

confidence: 99%

Evaluation of electron beam‐induced crosslinking of poly(ε‐caprolactone)—Effect of elevated temperatures

Huang

Gohs²,

Müller

et al. 2019

J of Applied Polymer Sci

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The effect of elevated temperatures (40, 80, and 170 °C) on the electron beam (EB)‐induced crosslinking and degradation of poly(ε‐caprolactone) (PCL) in nitrogen atmosphere without adding crosslinking agents has been investigated. The increased mass average molecular mass of EB modified masticated PCL (mPCL) samples were measured by size exclusion chromatography. The sol–gel investigation showed that mPCL tended to dominating crosslinking at a temperature of 170 °C. At this temperature, the maximum gel content of 56% was observed for an EB treatment with a dose of 100 kGy. In addition, the ratio of chain scission density to density of crosslinked units amounted to 0.25. The dynamic rheological test demonstrated that EB treatment at 170 °C led to the most remarkable elasticity enhancement behavior with enhanced viscosity, modulus, and decreased loss factor (tan δ). The occurrence of branching structure was evidently detected by van Gurp–Palmen plot in combination with the Cole–Cole plot for dose values ≤50 kGy. Less δ and a broadened relaxation process can be observed due to the introduction of some branched structures into mPCL. The results of enhanced molar mass and gel content analysis were confirmed by the dynamic rheological measurements. © 2019 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2019, 136, 47866.

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Subcritical carbon dioxide foaming of polycaprolactone for bone tissue regeneration

Cited by 21 publications

References 58 publications

Functional evaluation and testing of a newly developed Teleost’s Fish Otolith derived biocomposite coating for healthcare

Functional evaluation and testing of a newly developed Teleost’s Fish Otolith derived biocomposite coating for healthcare

Advancing spinal fusion: Interbody stabilization by in situ foaming of a chemically modified polycaprolactone

Evaluation of electron beam‐induced crosslinking of poly(ε‐caprolactone)—Effect of elevated temperatures

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