Subchondral bone trauma causes cartilage matrix degeneration: an immunohistochemical analysis in a canine model

Mrosek, Eike; Lahm, A.; Erggelet, Christoph; Uhl, Markus; Kurz, Haymo; Eissner, B.; Schagemann, Jan C.

doi:10.1016/j.joca.2005.08.004

Cited by 64 publications

(58 citation statements)

References 32 publications

(22 reference statements)

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“…Subchondral bone and articular cartilage are intimately related, such that alterations in one can affect the structure and functional integrity of the other (13,46). Surgical injury to either the subchondral bone or articular cartilage can lead to OA (13,46). Alterations in the subchondral bone can compromise the mechanical properties of the bone and change the distribution of force experienced by the cartilage (15,47).…”

Section: Discussionmentioning

confidence: 99%

“…Alterations in the subchondral bone can compromise the mechanical properties of the bone and change the distribution of force experienced by the cartilage (15,47). In this manner, abnormal impact loading and sheer forces may alter the capacity of chondrocytes to maintain cartilage integrity or may even induce chondrocyte apoptosis (13). In addition, disturbances of subchondral bone may promote the release of cytokines, metalloproteinases, and growth factors, each of which may contribute to cartilage alterations (12).…”

Section: Discussionmentioning

confidence: 99%

“…This is thought to relate to augmentation of the mechanical stress on the cartilage when these structures are dysfunctional (8). It has also been suggested that alterations in the subchondral bone can lead to OA (9)(10)(11)(12)(13). However, the nature and quality of the bone in OA have not been determined (14,15).…”

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confidence: 99%

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A type I collagen defect leads to rapidly progressive osteoarthritis in a mouse model

Blair-Levy

Watts

Fiorientino

et al. 2008

Arthritis & Rheumatism

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Objective. This study was undertaken to test the hypothesis that abnormalities of the subchondral bone can result in osteoarthritis (OA).Methods. We used a knockin model of human osteogenesis imperfecta, the Brittle IV (Brtl) mouse, in which defective type I collagen is expressed in bone. OA in individual mice was documented by micro-magnetic resonance imaging (micro-MRI) and micro-computed tomography (micro-CT). Alterations in the knee joints were confirmed by histopathologic and immunohistochemical analysis. In addition, atomic force microscopy (AFM) was used to assess the ultrastructure of the articular cartilage and subchondral bone matrix.Results. Brtl mice had decreased integrity of bone but initially normal articular cartilage. However, by the second month of life, Brtl mice developed alterations of the cartilage that were characteristic of OA, as documented by micro-CT, micro-MRI, and histologic evaluation. In addition, chondrocyte loss and breakdown of the collagen matrix in the residual cartilage were demonstrated using AFM.Conclusion. The Brtl mouse model demonstrates that progressive destruction of articular cartilage characteristic of OA may be secondary to altered architecture of the underlying subchondral bone.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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A type I collagen defect leads to rapidly progressive osteoarthritis in a mouse model

Blair-Levy

Watts

Fiorientino

et al. 2008

Arthritis & Rheumatism

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“…OA has been defined by the American College of Rheumatology (ACR) as a 'heterogeneous group of conditions that leads to joint symptoms and signs which are associated with defective integrity of articular cartilage, in addition to related changes in the underlying bone at the joint margins'. It remains an open question whether the initial changes take place in cartilage or bone or other articular tissues and, more importantly, what the causal relationship between these changes is (5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19) narrowing; JSW, joint space width; K, knee; KLG, Kellgren Lawrence grade; KOSS, knee osteoarthritis scoring system; LF, lateral femoral condyle; LT, lateral tibia; MRI, magnetic resonance imaging; MF, medial femoral condyle; MT, medial tibia; OA, osteoarthritis; OAI, Osteoarthritis Initiative; P, patella; PGs, proteoglycans; pLF, posterior aspect of the lateral femoral condyle; pMF, posterior aspect of the medial femoral condyle; qMRI, quantitative magnetic resonance imaging; SNR, signalto-noise ratio; SPGR, spoiled gradient echo; SSFP,steady-state free precession; T, tibia; T 1 , longitudinal relaxation time; T 1rho , T 1 in the rotating frame; T 2 , transverse relaxation time; tAB, total area of subchondral bone; ThC, thickness of the cartilage; ThCcAB, thickness of the cartilage, summarized over cAB (without including denuded areas as 0 mm cartilage thickness); ThCtAB, thickness of the cartilage, summarized over tAB (including denuded areas as 0 mm cartilage thickness); TKA, total knee arthroplasty; TrF, trochlear of femur; VC, volume of cartilage; VctAB, volume of cartilage normalized to the total area of subchondral bone (tAB); we, water excitation; WOMAC, Western Ontario McMaster University-Index; WORMS, whole-organ MRI score; mCT, micro-computed tomography.…”

Section: Rationale For Quantitative Mri Of Cartilage and Bone In Ostementioning

confidence: 99%

Quantitative MRI of cartilage and bone: degenerative changes in osteoarthritis

2006

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Magnetic resonance imaging (MRI) and quantitative image analysis technology has recently started to generate a great wealth of quantitative information on articular cartilage and bone physiology, pathophysiology and degenerative changes in osteoarthritis. This paper reviews semiquantitative scoring of changes of articular tissues (e.g. WORMS ¼ whole-organ MRI scoring or KOSS ¼ knee osteoarthritis scoring system), quantification of cartilage morphology (e.g. volume and thickness), quantitative measurements of cartilage composition (e.g. T 2 , T 1rho , T 1Gd ¼ dGEMRIC index) and quantitative measurement of bone structure (e.g. app. BV/TV, app. TbTh, app. Tb.N, app. Tb.Sp) in osteoarthritis. For each of these fields we describe the hardware and MRI sequences available, the image analysis systems and techniques used to derive semiquantitative and quantitative parameters, the technical accuracy and precision of the measurements reported to date and current results from cross-sectional and longitudinal studies in osteoarthritis. Moreover, the paper summarizes studies that have compared MRI-based measurements with radiography and discusses future perspectives of quantitative MRI in osteoarthritis. In summary, the above methodologies show great promise for elucidating the pathophysiology of various tissues and identifying risk factors of osteoarthritis, for developing structure modifying drugs (DMOADs) and for combating osteoarthritis with new and better therapy. Copyright # 2006 John Wiley & Sons, Ltd.KEYWORDS: osteoarthritis; joint; cartilage; bone; magnetic resonance imaging; knee; image analysis; disease-modifying drugs RATIONALE FOR QUANTITATIVE MRI OF CARTILAGE AND BONE IN OSTEOARTHRITISThe health and integrity of diarthrodial (synovial) joints are prerequisites for pain-free movement and have a major impact on the quality of life. However, the population of individuals above the age of 65 years is rapidly growing and 70% of the women and 60% of the men aged 65 years or older suffer from degenerative joint disease [¼ osteoarthritis (OA)] (1-7). OA has been defined by the American College of Rheumatology (ACR) as a 'heterogeneous group of conditions that leads to joint symptoms and signs which are associated with defective integrity of articular cartilage, in addition to related changes in the underlying bone at the joint margins'. It remains an open question whether the initial changes take place in cartilage or bone or other articular tissues and, more importantly, what the causal relationship between these changes is (5-19). Also, within each NMR IN BIOMEDICINE NMR Biomed. 2006; 19: 822-854 Published online in Wiley InterScience (www.interscience.wiley.com). DOI:10.1002/nbm.1063 narrowing; JSW, joint space width; K, knee; KLG, Kellgren Lawrence grade; KOSS, knee osteoarthritis scoring system; LF, lateral femoral condyle; LT, lateral tibia; MRI, magnetic resonance imaging; MF, medial femoral condyle; MT, medial tibia; OA, osteoarthritis; OAI, Osteoarthritis Initiative; P, patella; PGs, proteoglycans;...

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“…Although radiography is a low cost and broadly accessible method for veterinarians, its sensitivity in the diagnosis of DJD is low compared with techniques such as arthroscopy (Arias et al, 2003), computed tomography and magnetic resonance imaging (Mrosek et al 2006;Martel-Pelletier et al 2008). These techniques allow visualization of intra-articular structures without superimposition of other tissues, and in the case of magnetic resonance imaging, is also possible to evaluate the cartilage and its possible cracks quantitatively (MartelPelletier et al 2008;Crema et al, 2011).…”

Section: Groupmentioning

confidence: 99%

Glucosamine and chondroitin sulfate in the repair of osteochondral defects in dogs - clinical-radiographic analysis

Eleotério

Borges

Pontes³

et al. 2012

Rev. Ceres

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RESUMO Sulfato de condroitina e glucosamina na reparação de defeitos osteocondrais em cães -análise clínico-radiográficaDentre os tratamentos propostos para a doença articular degenerativa (DAD), os nutracêuticos condroprotetores à base de sulfato de condroitina constituem uma terapia não invasiva que favorece a manuteção da saúde da cartilagem. Além de utilizados em humanos, foram também disponibilizados para uso veterinário administrados na forma de suplemento nutricional independentemente de prescrição, uma vez que possuem somente o registro do Serviço de Inspeção Federal, que não exige testes de eficácia e segurança. A falta desses testes pelo Ministério da Agricultura Glucosamine and chondroitin sulfate in the repair of osteochondral defects in dogs -clinical-radiographic analysis 1Among the proposed treatments to repair lesions of degenerative joint disease (DJD), chondroprotective nutraceuticals composed by glucosamine and chondroitin sulfate are a non-invasive theraphy with properties that favors the health of the cartilage. Although used in human, it is also available for veterinary use with administration in the form of nutritional supplement independent of prescription, since they have registry only in the Inspection Service, which does not require safety and efficacy testing. The lack of such tests to prove efficacy and safety of veterinary medicines required by the Ministry of Agriculture and the lack of scientific studies proving its benefits raises doubts about the efficiency of the concentrations of such active substances. In this context, the objective of this study was to evaluate the efficacy of a veterinary chondroprotective nutraceutical based on chondroitin sulfate and glucosamine in the repair of osteochondral defects in lateral femoral condyle of 48 dogs, through clinical and radiographic analysis. The animals were divided into treatment group (TG) and control group (CG), so that only the TG received the nutraceutical every 24 hours at the rate recommended by the manufacturer. The results of the four treatment times (15, 30, 60 and 90 days) showed that the chondroprotective nutraceutical, in the rate, formulation and administration at the times used, did not improve clinical signs and radiologically did not influence in the repair process of the defects, since the treated and control groups showed similar radiographic findings at the end of the treatments.

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Subchondral bone trauma causes cartilage matrix degeneration: an immunohistochemical analysis in a canine model

Cited by 64 publications

References 32 publications

A type I collagen defect leads to rapidly progressive osteoarthritis in a mouse model

A type I collagen defect leads to rapidly progressive osteoarthritis in a mouse model

Quantitative MRI of cartilage and bone: degenerative changes in osteoarthritis

Glucosamine and chondroitin sulfate in the repair of osteochondral defects in dogs - clinical-radiographic analysis

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