SU-8-based Flexible Amperometric Device with IDA Electrodes to Regenerate Redox Species in Small Spaces

Kanno, Yusuke; Goto, Takehito; Ino, Kosuke; Inoue, Kumi Y.; Takahashi, Yasufumi; Shiku, Hitoshi; Matsue, Tomokazu

doi:10.2116/analsci.30.305

Cited by 9 publications

(5 citation statements)

References 21 publications

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“…Redox currents from the top and bottom ring electrodes were calculated using COMSOL Multiphysics (version 5.1, COMSOL, Inc., USA). 24,34,35 A three-dimensional model containing the nanocavities, ring electrodes and microwells is described in Fig. S2 and S3.…”

Section: Redox Current Simulationmentioning

confidence: 99%

A local redox cycling-based electrochemical chip device with nanocavities for multi-electrochemical evaluation of embryoid bodies

et al. 2015

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An electrochemical device, which consists of electrode arrays, nanocavities, and microwells, was developed for multi-electrochemical detection with high sensitivity. A local redox cycling-based electrochemical (LRC-EC) system was used for multi-electrochemical detection and signal amplification. The LRC-EC system consists of n(2) sensors with only 2n bonding pads for external connection. The nanocavities fabricated in the sensor microwells enable significant improvement of the signal amplification compared with the previous devices we have developed. The present device was successfully applied for evaluation of embryoid bodies (EBs) from embryonic stem (ES) cells via electrochemical measurements of alkaline phosphatase (ALP) activity in the EBs. In addition, the EBs were successfully trapped in the sensor microwells of the device using dielectrophoresis (DEP) manipulation, which led to high-throughput cell analysis. This device is considered to be useful for multi-electrochemical detection and imaging for bioassays including cell analysis.

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Section: Redox Current Simulationmentioning

confidence: 99%

A local redox cycling-based electrochemical chip device with nanocavities for multi-electrochemical evaluation of embryoid bodies

et al. 2015

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“…The development of several types of electrochemical devices for bioassay applications, including analyses of yeast and mammalian cells, has been reported. [29][30][31][32][33][34][35][36][37][38][39][40][41][42][43][44] IDA electrodes were incorporated to amplify signals from cells and provide highly sensitive assays. Yeast cellbased IDA electrodes were utilized for the detection of hormone active chemicals.…”

Section: Chip-based Devices For Electrochemical Bioanalysismentioning

confidence: 99%

Microchemistry- and MEMS-based Integrated Electrochemical Devices for Bioassay Applications

Ino

2015

Electrochemistry

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Here, we present an overview of our recent research progress in development of electrochemical devices/systems for bioassays. These devices/systems are based on microchemistry and micro-electromechanical systems (MEMS) for sophisticated analytical and electrochemical measurements. In particular, we exploit the unique chemical reactions occurring in micrometer-size spaces and/or involving micrometer-size structures for bioassays, including cell analyses. This paper addresses five topics: probe-, chip-, large-scale-integration chip-, and dielectrophoresisbased devices, and electrodeposition of hydrogels for bioassays.

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“…In addition, cell surface topography can be monitored at the nanometer scale, 9 and integrated electrodes on a chip, such as an interdigitated electrode array (IDA), can be used for signal amplification. 10 Moreover, integrated electrodes on a chip can be used for cell manipulation and cell separation by inducing dielectrophoresis (DEP). 11,12 In addition, highthroughput analysis and real-time electrochemical imaging have been performed using electrode arrays, 13 including complementary metal-oxide semiconductor (CMOS) sensors [14][15][16][17][18] and charge-coupled device (CCD) sensors.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Imaging of Cell Activity in Hydrogels Embedded in Grid-shaped Polycaprolactone Scaffolds Using a Large-scale Integration-based Amperometric Device

et al. 2018

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Tissue engineering requires analytical methods to monitor cell activity in hydrogels. Here, we present a method for the electrochemical imaging of cell activity in hydrogels embedded in printed polycaprolactone (PCL) scaffolds. Because a structure made of only hydrogel is fragile, PCL frameworks are used as a support material. A grid-shaped PCL was fabricated using an excluder printer. Photocured hydrogels containing cells were set at each grid hole, and cell activity was monitored using a large-scale integration-based amperometric device. The electrochemical device contains 400 microelectrodes for biomolecule detection, such as dissolved oxygen and enzymatic products. As proof of the concept, alkaline phosphatase and respiration activities of embryonic stem cells in the hydrogels were electrochemically monitored. The results indicate that the electrochemical imaging is useful for evaluating cells in printed scaffolds.

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SU-8-based Flexible Amperometric Device with IDA Electrodes to Regenerate Redox Species in Small Spaces

Cited by 9 publications

References 21 publications

A local redox cycling-based electrochemical chip device with nanocavities for multi-electrochemical evaluation of embryoid bodies

A local redox cycling-based electrochemical chip device with nanocavities for multi-electrochemical evaluation of embryoid bodies

Microchemistry- and MEMS-based Integrated Electrochemical Devices for Bioassay Applications

Electrochemical Imaging of Cell Activity in Hydrogels Embedded in Grid-shaped Polycaprolactone Scaffolds Using a Large-scale Integration-based Amperometric Device

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