A local redox cycling-based electrochemical chip device with nanocavities for multi-electrochemical evaluation of embryoid bodies

Kanno, Yusuke; Ino, Kosuke; Shiku, Hitoshi; Matsue, Tomokazu

doi:10.1039/c5lc01016k

Cited by 33 publications

(37 citation statements)

References 55 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Furthermore, recent advances in electrochemical methods have created innovative routes for amplifying the signal of biorecognition events . One such method is redox‐cycling, which is capable of resolving signals from single molecules . The principle of redox‐cycling amplification is based on repeating oxidation and reduction events of redox‐active molecules between two closely spaced and independently biased electrodes.…”

Section: Introductionmentioning

confidence: 99%

Flexible Microgap Electrodes by Direct Inkjet Printing for Biosensing Application

et al. 2017

View full text Add to dashboard Cite

A rapid fabrication method of microgap electrodes using inkjet printing is described. In this approach, the lateral spacing between two printed electrode lines is precisely controlled down to 1 µm without any surface modification or substrate patterning. The strong confinement, well below typical resolution of inkjet printing, relies on complete solvent evaporation between the printing of adjacent electrode structures, which is achieved by controlling the printing speed and temperature profiles. The feasibility of this method is demonstrated by writing electrode structures with two distinct inks, based on carbon and silver nanoparticles, with comparable results. As an application proof-of-principle, arrays of microgap electrodes are fabricated using a carbon nanoparticle ink for electrochemical detection based on redox-cycling, a technique in which the sensitivity of the device depends on the distance between the two electrodes. The redox-cycling amplification of electrochemical signals is demonstrated and it is shown that the printed microgap device can be used as an electrochemical biosensor for the determination of human immunodeficiency virus (HIV)-related single-stranded DNA. This work presents a promising new approach for fabricating low-cost and label-free redox-cycling biosensors using all-inkjet-printed electrodes. www.adv-biosys.com

show abstract

Section: Introductionmentioning

confidence: 99%

Flexible Microgap Electrodes by Direct Inkjet Printing for Biosensing Application

et al. 2017

View full text Add to dashboard Cite

show abstract

“…In addition, electrochemical methods can be applied to turbid and colored solutions as well as for cell suspensions. Using these techniques, a variety of systems employing electrodes as probes and chips for the amperometric detection of neurotransmitter release have been implemented. Although fast‐scan cyclic voltammetry techniques (FSCV) have been applied to selective and simultaneous detection of multiple analytes their use is limited due to the small numbers (≤16) of probes for FSCV.…”

Section: Figurementioning

confidence: 99%

Simultaneous and Selective Imaging of Dopamine and Glutamate Using an Enzyme‐modified Large‐scale Integration (LSI)‐based Amperometric Electrochemical Device

et al. 2018

Self Cite

View full text Add to dashboard Cite

Since neurotransmitters play critical roles in nervous systems, the imaging techniques that can visualize them are needed. This communication describes the development of a method for simultaneous imaging of dopamine (DA) and glutamate (Glu), typical neurotransmitters, using enzyme‐modified large‐scale integration (LSI)‐based amperometric devices, referred to “Bio‐LSI”. The DA was detected by using direct electrochemical oxidation while Glu was measured by using an indirect electrochemical reaction involving glutamate oxidase (GluOx)/horseradish peroxidase (HRP) and osmium (Os) polymer‐modified electrodes. The Os‐HRP polymer was modified on designated sensor electrodes through electrodeposition. The selective and simultaneous imaging of neurotransmitters such as DA and Glu was achieved by applying the enzyme‐modified Bio‐LSI.

show abstract

“…12 shows the manipulation of the EBs of ES cells using positive DEP ( pDEP). 70 As shown in Fig. 12A, an AC electric field was applied between the top and bottom electrodes to guide an EB into the microwell by DEP (Fig.…”

Section: Cell Manipulation Using Dielectrophoresis (Dep)mentioning

confidence: 99%

Micro/nanoelectrochemical probe and chip devices for evaluation of three-dimensional cultured cells

Ino

Şen

Shiku

et al. 2017

Analyst

Self Cite

View full text Add to dashboard Cite

Herein, we present an overview of recent research progress in the development of micro/nanoelectrochemical probe and chip devices for the evaluation of three-dimensional (3D) cultured cells. First, we discuss probe devices: a general outline, evaluation of O 2 consumption, enzyme-modified electrodes, evaluation of endogenous enzyme activity, and the collection of cell components from cell aggregates are discussed. The next section is focused on integrated chip devices: a general outline, electrode array devices, smart electrode array devices, droplet detection of 3D cultured cells, cell manipulation using dielectrophoresis (DEP), and electrodeposited hydrogels used for fabrication of 3D cultured cells on chip devices are discussed. Finally, we provide a summary and discussion of future directions of research in this field.

show abstract

A local redox cycling-based electrochemical chip device with nanocavities for multi-electrochemical evaluation of embryoid bodies

Cited by 33 publications

References 55 publications

Flexible Microgap Electrodes by Direct Inkjet Printing for Biosensing Application

Flexible Microgap Electrodes by Direct Inkjet Printing for Biosensing Application

Simultaneous and Selective Imaging of Dopamine and Glutamate Using an Enzyme‐modified Large‐scale Integration (LSI)‐based Amperometric Electrochemical Device

Micro/nanoelectrochemical probe and chip devices for evaluation of three-dimensional cultured cells

Contact Info

Product

Resources

About