Micro/nanoelectrochemical probe and chip devices for evaluation of three-dimensional cultured cells

Ino, Kosuke; Şen, Mustafa; Shiku, Hitoshi; Matsue, Tomokazu

doi:10.1039/c7an01442b

Cited by 35 publications

(26 citation statements)

References 89 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the near future, this imaging technique will be used to monitor the release of dopamine from cells in the presence of ascorbic acid without potential scanning, e.g., by fast-scan voltammetry, although the interaction between dopamine and ascorbic acid during electrochemical detection would have to be carefully investigated. Previously, electrode arrays were applied to three-dimensional tissue organs; hence, (23)(24)(25)(26) we also aim to investigate the current system for possible application in this regard, such as in organs-on-a-chip. Figure 6(a) shows amperograms for various concentrations of PAP and PAPP.…”

Section: Resultsmentioning

confidence: 99%

Differential Electrochemicolor Imaging Using LSI-based Device for Simultaneous Detection of Multiple Analytes

Ino¹,

Onodera²,

Nashimoto³

et al. 2019

Sensors and Materials

Self Cite

View full text Add to dashboard Cite

Section: Resultsmentioning

confidence: 99%

Differential Electrochemicolor Imaging Using LSI-based Device for Simultaneous Detection of Multiple Analytes

Ino¹,

Onodera²,

Nashimoto³

et al. 2019

Sensors and Materials

Self Cite

View full text Add to dashboard Cite

“…For example, cell differentiation of embryonic stem cells and early‐stage bone differentiation have been electrochemically detected. Several types of integrated electrochemical devices have been developed for bioanalysis including cell analysis and they are discussed in detail elsewhere . The electrochemical approach can be utilized for organ transplantation and quality assurance of stem cells.…”

Section: Electrochemical Intracellular Sensing In Situmentioning

confidence: 99%

Intracellular Electrochemical Sensing

et al. 2018

Self Cite

View full text Add to dashboard Cite

Observing biochemical processes within living cell is imperative for biological and medical research. Fluoresce imaging is widely used for intracellular sensing of cell membranes, nuclei, lysosomes, and pH. Electrochemical assays have been proposed as an alternative to fluorescence‐based assays because of excellent analytical features of electrochemical devices. Notably, thanks to the rapid progress of micro/nanotechnologies and electrochemical techniques, intracellular electrochemical sensing is making rapid progress, leading to a successful detection of intracellular components. Such insight can provide a deep understanding of cellular biological processes and, ultimately, define the human healthy and diseased states. In this review, we present an overview of recent research progress in intracellular electrochemical sensing. We focus on two main topics, electrochemical extraction of cytosolic contents from cells and intracellular electrochemical sensing in situ.

show abstract

“…5,16 For non-scanning electrochemical bio-devices, electrode arrays can be integrated on a cellular chip for high-throughput real time monitoring of biological functions. [17][18][19][20][21][22] Previously, we have reviewed progress of whole-cell biosensors, especially incorporation of microfabrication technologies and genemodified engineering. 23 Originally, a cell-based sensor meant an electrode immobilized with whole-cells instead of purified enzymes.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Biosensing System for Single Cells, Cellular Aggregates and Microenvironments

Shiku

2018

ANAL. SCI.

Self Cite

View full text Add to dashboard Cite

Applications of electrochemical biosensing for surveying intact cells and tissues have been focus of attention. Two experimental approaches have been used when performing amperometric measurements on biological cells, the stylustype microelectrode probes and the electrode-integrated microdevices based on lithographic technologies. For the probe scanning approach, various types of microsensors were developed to monitor localized physical or chemical natures at a variety of surfaces in situ under wet conditions. Scanning electrochemical microscopy (SECM) has been applied for monitoring local oxygen, enzyme activity, and collection of transcripts. For the non-scanning type of approach, electrode array devices allow very rapid response, parallel monitoring, and multi-analyte assay. Sveral topics of on-chip-culture system were introduced especially concerning on gene expression monitoring by reporter system and reconstruction of in vivo-like nature by controlling microenvironments. Electrochemical reporter assay has been demonstrated to monitor the gene expression process of the gene-modified cultured cells. Long-term monitoring of cellular function of spheroids and three dimensionally-cultured cells were carried out by controlling microenvironments on the cellular chip.

show abstract

Micro/nanoelectrochemical probe and chip devices for evaluation of three-dimensional cultured cells

Cited by 35 publications

References 89 publications

Differential Electrochemicolor Imaging Using LSI-based Device for Simultaneous Detection of Multiple Analytes

Differential Electrochemicolor Imaging Using LSI-based Device for Simultaneous Detection of Multiple Analytes

Intracellular Electrochemical Sensing

Electrochemical Biosensing System for Single Cells, Cellular Aggregates and Microenvironments

Contact Info

Product

Resources

About