A study was conducted to determine the fungicidal activity of a sanitary contaminant of feeds – representatives of the genus Aspergillus Mich. – species of Aspergillus flavus using the ozone treatment method, treatment with the composition of Ag and Cu nanoparticles and the combined effect of these methods. When studying the combined effect of ozone, NPAg and NPCu, it was found that at the concentration ratio of 100:100 μg/cm3, there was a fungicidal effect on museum strains of Aspergillus flavus test cultures, i.e., the combination with ozone enhanced the antifungal properties of nanocomplex.