Study on the relationship between the expression of &lt;I&gt;BPI&lt;/I&gt; gene and &lt;I&gt;Escherichia coli&lt;/I&gt; F18 infection in piglets

Ye, Lan; Chen, Zi; Liu, Lu; Zhu, Jing; Xie, Kaizhou; Zhu, Guo-Qian; Huang, Xiao-Guo; Bao, Wenbin; Wu, Shenglong; Wang, Jinyu

doi:10.3724/sp.j.1005.2011.01225

Cited by 5 publications

(5 citation statements)

References 14 publications

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“…These results suggested that BPI is likely related to the intestinal infection caused by ETEC F18. This increased expression of BPI in the intestine may be connected to resistance to ETEC F18 (Ye et al, 2011). Coddens et al (2007) reported that the expression level of the ETEC F18 strain receptor increases with piglet age during the first 3 weeks after birth and that expression is maintained in older pigs, which is consistent with the BPI expression levels in our studies.…”

Section: Discussionsupporting

confidence: 81%

“…Christopher et al (2004) have identified BPI as the candidate gene for the susceptibility of swine to Salmonella. Differential expression of BPI between enterotoxigenic Escherichia coli F18 (ETEC F18)-resistant and ETEC F18-sensitive piglets was analyzed in our preliminary study, and we determined that expression of BPI is significantly high only in the duodenum and jejunum of pigs, suggesting that increased expression of BPI in the intestines is connected to resistance to ETEC F18 (Ye et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

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Age-dependent expression of the BPI gene in Sutai piglets

Zhu¹,

Chen²,

Wu³

et al. 2013

Genet. Mol. Res.

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ABSTRACT. We compared and analyzed expression of the BPI gene of Sutai piglets ranging from newborn to post-weaning days 8, 18, 30, and 35 by the real-time PCR method, in order to determine if it is involved in protection against disease caused by ETEC F18. There was a significant difference between 18-and 35-day expression in the jejunum. There were also significant differences between 35-day expression and expression at the other development stages in the duodenum. There were no significant differences in expression at 8, 18, and 30 days in the jejunum. We conclude that the porcine BPI gene may be the direct factor that caused resistance to ETEC F18 in weaning piglets, and that the resistance to ETEC F18 in weaning piglets is related to upregulation of mRNA expression of the BPI gene to a certain extent.

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Section: Discussionsupporting

confidence: 81%

Section: Introductionmentioning

confidence: 99%

Age-dependent expression of the BPI gene in Sutai piglets

Zhu¹,

Chen²,

Wu³

et al. 2013

Genet. Mol. Res.

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“…a higher immune response capability, which may be a potential molecular marker for piglets against E. coli F18. Meanwhile, Ye et al (17) further reported that high expression of the AA genotype is in connection with special disease resistance by detecting BPI gene expression in Sutai pigs with different genotypes, consistent with the results of Liu et al (16). With respect to genetic variation analysis of the exon 10 polymorphisms of the BPI gene among different pig breeds, the results suggested that individuals with the AA genotype may have high immune response ability against gram-negative bacteria.…”

Section: Discussionsupporting

confidence: 75%

Polymorphisms of exon 10 in the BPI gene and its association analysis with some reproductive traits and partial immune indexes in Meishan pigs

Qu¹,

Wei²,

Ye³

et al. 2019

Turk J Vet Anim Sci

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This study was conducted to detect polymorphisms of BPI gene exon 10 by PCR-RFLP in Meishan pigs and their associations with some important cytokine levels (IL-1β, IL-4, IL-6, IL-8, IL-10, TGF-1β, IFN-γ, TNF-α) and reproductive traits (total number born (TNB), number born alive (NBA), birth litter weight (BLW), and weaning litter weight (WLW)), with the aim of identifying effective genetic markers for molecular breeding. The results revealed three genotypes, including AA, AB, and BB, with the trend of AA > BB > AB in reproductive traits. Pigs with the AA genotype were significantly higher than the AB genotype in TNB and IFN-γ (P < 0.05), and extremely significantly higher in NBA and WLW (P < 0.01). Moreover, TGF-1β and TNF-α levels were significantly higher in the AA genotype than the BB genotype (P < 0.05). However, there were no significant differences among the three genotypes in the levels of other cytokines (P > 0.05). Comprehensive analysis indicated that the polymorphisms of exon 10 in the BPI gene have significant genetic effects on some reproductive traits and cytokines in Meishan pigs, for which the AA genotype was demonstrated to be the favorable genotype, which could be used as potential genetic markers for in-depth research and examination.

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“…Using these populations, we analyzed the expression level of the porcine BPI gene in vivo. Ye et al (2011) analyzed the differential expression between E. coli F18-resistant and sensitive piglets using real-time PCR. The results showed that expression of BPI gene was extremely low or undetectable in various tissues, including the heart, liver, spleen, lung, kidney, stomach, muscle, thymus, and lymph nodes.…”

Section: Discussionmentioning

confidence: 99%

“…In previous studies, based on the FUT1 genetic marker, we established E. coli F18-resistant and -susceptible resource populations in Sutai pigs. Differential expression of BPI between enterotoxigenic E. coli F18 (ETEC F18)-resistant and ETEC F18-sensitive piglets was analyzed in our preliminary study, and we determined that expression of BPI is significantly high only in the duodenum and jejunum of pigs, suggesting that increased expression of BPI in the intestines is related to the resistance to ETEC F18 (Ye et al, 2011). Moreover, Zhu et al (2013) observed significant differences between 35-day expression and expression during other development stages in the duodenum by analyzing the age-dependent tissue expression of porcine BPI in Sutai pigs (Zhu et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Identification of BPI protein produced in different expression system and its association with Escherichia coli F18 susceptibility

Liu

Dong

et al. 2015

Genet. Mol. Res.

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ABSTRACT. The super antibiotic bactericidal/permeability-increasing (BPI) protein is a member of a new generation of proteins that have been implicated as endotoxin-neutralizing agents. In this study, recombinant porcine BPI protein was obtained by generating porcine BPI encoding prokaryotic, eukaryotic, and yeast expression vectors. Recombinant protein expression was detected in yeast GS115, Escherichia coli, and 293-6E cells by gel electrophoresis and Western blotting. Escherichia coli F18 is the primary Gram-negative bacteria in the gut and the main pathogen leading to diarrhea and edema disease in weaning piglets. Therefore, E. coli F18-resistant and -sensitive Sutai piglets were used to test differential expression of BPI protein by Western blotting and to investigate the potential correlation between BPI protein expression and E. coli F18-susceptibility. Recombinant porcine BPI protein expression was not detected in the prokaryotic and yeast expression systems; however, soluble protein was detected in the eukaryotic expression system. These data indicate the strong bacterio- static action of the BPI protein and confirm the feasibility of obtaining large amounts of recombinant porcine BPI recombinant protein using this eukaryotic expression system. In addition, the BPI protein expression levels in the E. coli F18-resistant group were significantly higher than those in the sensitive group, indicating that high BPI protein expression is associated with resistance to E. coli F18. Our findings provide a basis for further investigations into the development of a drug designed to confer resistance to E. coli F18 in weaning piglets.

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Study on the relationship between the expression of <I>BPI</I> gene and <I>Escherichia coli</I> F18 infection in piglets

Cited by 5 publications

References 14 publications

Age-dependent expression of the BPI gene in Sutai piglets

Age-dependent expression of the BPI gene in Sutai piglets

Polymorphisms of exon 10 in the BPI gene and its association analysis with some reproductive traits and partial immune indexes in Meishan pigs

Identification of BPI protein produced in different expression system and its association with Escherichia coli F18 susceptibility

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