MicroRNAs (miRNAs) have recently been recognized as targets for anti-metastatic therapy against cancer malignancy. Development of effective miRNA mediated therapies remains a challenge to both basic research and clinical practice. Here we presented the evidence for a miR-708-5p mediated replacement therapy against metastatic lung cancer. Expression of miR-708-5p was substantially reduced in metastatic lung cancer samples and cancer cell lines when compared to non-metastatic counterparts. Expression of the miRNA suppressed cell survival and metastasis in vitro through its direct target p21, and inhibited the PI3K/AKT pathway and stem cell-like characteristics of lung cancer cells. Systemic administration of this miRNA in a mouse model of NSCLC using polyethylenimine (PEI)-mediated delivery of unmodified miRNA mimics induced tumor specific apoptosis. It also effectively protected the tested animals from developing metastatic malignancy without causing any observed toxicity.The findings strongly support miR-708-5p as a novel and effective therapeutic agent against metastatic malignancy of non-small cell lung cancer.
A strategy was developed to couple
photocatalytic oxidation with
photocatalytic reduction technology to realize one-pot conversion
of MB into hydrocarbons for the first time. In this approach, organic
pollutants were first decomposed into CO2 by photodegradation
and then the as-obtained CO2 was converted into CH3OH, C2H5OH, and CH4 through
photocatalytic reduction of CO2 under solar spectrum irradiation
by using GQDs/V-TiO2 catalysts. The experimental results
show that 5%GQDs/V-TiO2 has the best photocatalytic activity
and the product rates of CH3OH, C2H5OH, and CH4 are 13.24, 5.65, and 0.445 μmol g–1 h–1, respectively. The corresponding
apparent quantum efficiency is 4.87% at 420 nm. The one-pot conversion
of MB into hydrocarbons was demonstrated by a series of experiments.
The photocatalytic mechanisms of one-pot conversion of MB into hydrocarbons
were proposed to explain the detailed photocatalytic process.
Aims: To establish a novel cell surface display system that would enable the display of target proteins on Lactobacillus plantarum.
Methods and Results: BlastP analysis of the amino acids sequence data revealed that the N‐terminus of the putative muropeptidase MurO from L. plantarum contained two putative lysin motif (LysM) repeat regions, implying that the MurO was involved in bacterial cell wall binding. To investigate the potential of MurO for surface display, green fluorescent protein (GFP) was fused to MurO at its C‐terminus and the resulting fusion protein was expressed in Escherichia coli. After being mixed with L. plantarum cells in vitro, GFP was successfully displayed on the surfaces of L. plantarum cells. Increases in the fluorescence intensities of chemically pretreated L. plantarum cells compared to those of nonpretreated cells suggested that the peptidoglycan was the binding ligand for MurO. SDS sensitivity assay showed that the GFP fluorescence intensity was reduced after being treated with SDS. To demonstrate the applicability of the MurO‐mediated surface display system, β‐galactosidase from Bifidobacterium bifidium, in place of GFP, was functionally displayed on the surface of L. plantarum cells via MurO.
Conclusions: The MurO was a novel anchor protein for constructing a surface display system for L. plantarum.
Significance and Impact of Study: The success in surface display of GFP and β‐galactosidase opened up the feasibility of employing the cell wall anchor of MurO for surface display in L. plantarum.
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