Age-dependent expression of the BPI gene in Sutai piglets

Zhu, Jing; Chen, Zi; Wu, Zhengchang; Liu, L.; Zheng, Xingguo; Su, Xianmin; Zhu, Guoqiang; Huang, Xiao-Guo; Wu, Shenglong; Bao, Wenbin

doi:10.4238/2012.april.12.1

Cited by 7 publications

(4 citation statements)

References 17 publications

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“…A similar situation has been observed in vitro for ETEC, with LT expression modified by pH [ 52 ]. Also, it has been demonstrated that the porcine intestinal epithelium can develop a resistance to F18-mediated adherence [ 53 ] and the stress of early weaning reduces the ability of piglets to cope with ETEC infection [ 54 ]. This data indicates that whilst it is important to characterise pathogenic E. coli , host characteristics and the intestinal environment may play a role in altering pathogenicity and account for the variation in disease severity between these isolates given their highly similar virulence factor content.…”

Section: Discussionmentioning

confidence: 99%

Comparative genomic analysis of a multiple antimicrobial resistant enterotoxigenic E. coli O157 lineage from Australian pigs

et al. 2015

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BackgroundEnterotoxigenic Escherichia coli (ETEC) are a major economic threat to pig production globally, with serogroups O8, O9, O45, O101, O138, O139, O141, O149 and O157 implicated as the leading diarrhoeal pathogens affecting pigs below four weeks of age. A multiple antimicrobial resistant ETEC O157 (O157 SvETEC) representative of O157 isolates from a pig farm in New South Wales, Australia that experienced repeated bouts of pre- and post-weaning diarrhoea resulting in multiple fatalities was characterized here. Enterohaemorrhagic E. coli (EHEC) O157:H7 cause both sporadic and widespread outbreaks of foodborne disease, predominantly have a ruminant origin and belong to the ST11 clonal complex. Here, for the first time, we conducted comparative genomic analyses of two epidemiologically-unrelated porcine, disease-causing ETEC O157; E. coli O157 SvETEC and E. coli O157:K88 734/3, and examined their phylogenetic relationship with EHEC O157:H7.ResultsO157 SvETEC and O157:K88 734/3 belong to a novel sequence type (ST4245) that comprises part of the ST23 complex and are genetically distinct from EHEC O157. Comparative phylogenetic analysis using PhyloSift shows that E. coli O157 SvETEC and E. coli O157:K88 734/3 group into a single clade and are most similar to the extraintestinal avian pathogenic Escherichia coli (APEC) isolate O78 that clusters within the ST23 complex. Genome content was highly similar between E. coli O157 SvETEC, O157:K88 734/3 and APEC O78, with variability predominantly limited to laterally acquired elements, including prophages, plasmids and antimicrobial resistance gene loci. Putative ETEC virulence factors, including the toxins STb and LT and the K88 (F4) adhesin, were conserved between O157 SvETEC and O157:K88 734/3. The O157 SvETEC isolate also encoded the heat stable enterotoxin STa and a second allele of STb, whilst a prophage within O157:K88 734/3 encoded the serum survival gene bor. Both isolates harbor a large repertoire of antibiotic resistance genes but their association with mobile elements remains undetermined.ConclusionsWe present an analysis of the first draft genome sequences of two epidemiologically-unrelated, pathogenic ETEC O157. E. coli O157 SvETEC and E. coli O157:K88 734/3 belong to the ST23 complex and are phylogenetically distinct to EHEC O157 lineages that reside within the ST11 complex.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-015-1382-y) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Comparative genomic analysis of a multiple antimicrobial resistant enterotoxigenic E. coli O157 lineage from Australian pigs

et al. 2015

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“…E. coli F18 is the primary causative of diarrhea and edematous disease which are two major causes of mortality in postweaning piglets, and these disease lead to huge economic losses in the swine industry [6]. Our preliminary study suggested that BPI expression is connected to resistance against E. coli F18 [7]. …”

Section: Introductionmentioning

confidence: 99%

Correlation between BPI Gene Upstream CpG Island Methylation and mRNA Expression in Piglets

Wang

Yin

Sun

et al. 2014

IJMS

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Diarrhea and edematous disease are two major causes of mortality in postweaning piglets, and these conditions lead to huge economic losses in the swine industry. E. coli F18 is the primary causative agent of these two diseases. Bactericidal/permeability-increasing protein (BPI) plays an important role in the natural defense of the host. The aim of this study was to determine the correlation between BPI gene upstream CpG island methylation and mRNA expression. In this study, bisulfite sequencing PCR (BSP) was used to detect the methylation status of the BPI gene upstream CpG island and fluorescence quantitative PCR was used to detect BPI expression in the duodenum of piglets from birth to weaning age. BPI upstream CpG islands were shown to have many putative transcription factor binding sites, 10 CpG sites and every CpG site was methylated. The CpG island methylation level was lowest in 30-day piglets and was significantly lower than levels in 8-day piglets (p < 0.05). BPI mRNA expression was significantly higher in 30-day piglets than at any other age (p < 0.05). Pearson’s correlation analysis showed that the methylation status of the CpG island was negatively correlated with BPI mRNA expression. Statistical significances were found in CpG_1, CpG_3, CpG_4, CpG_7 and CpG_10 (p < 0.05). The data indicate that BPI expression is improved by demethylation of the BPI gene upstream CpG island. Furthermore, CpG_1, CpG_3, CpG_4, CpG_7 and CpG_10 may be critical sites in the regulation of BPI gene expression.

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“…The results of this study showed that expression of the BPI gene in the duodenum and jejunum of Sutai piglets was significantly higher than that in Yorkshire and Meishan piglets. In a previous study, we analyzed BPI gene expression in Sutai piglets over a time period ranging from newborns to post-weaning days 8, 18, 30, and 35 (Zhu et al, 2013). There were significant differences between 35-day expression and expression at other developmental stages in the duodenum, with the greatest vulnerability to E. coli F18 infection detected at 35 days of age.…”

Section: Discussionmentioning

confidence: 99%

“…Differential expression of BPI between enterotoxigenic E. coli F18 (ETEC F18)-resistant and ETEC F18-sensitive piglets was analyzed in our preliminary study, and we determined that expression of BPI is significantly high only in the duodenum and jejunum of pigs, suggesting that increased expression of BPI in the intestines is related to the resistance to ETEC F18 (Ye et al, 2011). Moreover, Zhu et al (2013) observed significant differences between 35-day expression and expression during other development stages in the duodenum by analyzing the age-dependent tissue expression of porcine BPI in Sutai pigs (Zhu et al, 2013). The greatest susceptibility to E. coli F18 infection was observed at 35 days of age, with predominant E. coli F18 colonization in the duodenum.…”

Section: Introductionmentioning

confidence: 99%

Identification of BPI protein produced in different expression system and its association with Escherichia coli F18 susceptibility

Liu

Dong

et al. 2015

Genet. Mol. Res.

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ABSTRACT. The super antibiotic bactericidal/permeability-increasing (BPI) protein is a member of a new generation of proteins that have been implicated as endotoxin-neutralizing agents. In this study, recombinant porcine BPI protein was obtained by generating porcine BPI encoding prokaryotic, eukaryotic, and yeast expression vectors. Recombinant protein expression was detected in yeast GS115, Escherichia coli, and 293-6E cells by gel electrophoresis and Western blotting. Escherichia coli F18 is the primary Gram-negative bacteria in the gut and the main pathogen leading to diarrhea and edema disease in weaning piglets. Therefore, E. coli F18-resistant and -sensitive Sutai piglets were used to test differential expression of BPI protein by Western blotting and to investigate the potential correlation between BPI protein expression and E. coli F18-susceptibility. Recombinant porcine BPI protein expression was not detected in the prokaryotic and yeast expression systems; however, soluble protein was detected in the eukaryotic expression system. These data indicate the strong bacterio- static action of the BPI protein and confirm the feasibility of obtaining large amounts of recombinant porcine BPI recombinant protein using this eukaryotic expression system. In addition, the BPI protein expression levels in the E. coli F18-resistant group were significantly higher than those in the sensitive group, indicating that high BPI protein expression is associated with resistance to E. coli F18. Our findings provide a basis for further investigations into the development of a drug designed to confer resistance to E. coli F18 in weaning piglets.

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Age-dependent expression of the BPI gene in Sutai piglets

Cited by 7 publications

References 17 publications

Comparative genomic analysis of a multiple antimicrobial resistant enterotoxigenic E. coli O157 lineage from Australian pigs

Comparative genomic analysis of a multiple antimicrobial resistant enterotoxigenic E. coli O157 lineage from Australian pigs

Correlation between BPI Gene Upstream CpG Island Methylation and mRNA Expression in Piglets

Identification of BPI protein produced in different expression system and its association with Escherichia coli F18 susceptibility

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