Study on occurrence and antibiogram pattern of Escherichia coli from raw milk samples in Anand, Gujarat, India

Abstract

Aim : The study was carried out with aim to isolate Escherichia coli from raw milk samples and determine antibiogram pattern of E. coli isolates. Materials and Methods: During 6 months duration of study a total of 100 raw milk samples were collected from different places in and around Anand city such as individual household, cattle farms, milk collection centres of Co-operative milk dairies and milk vendors. All raw milk samples were enriched in peptone water and inoculated on selective media and various biochemical tests were performed for confirmation of isolates. Antibiogram pattern of E.coli to antimicrobial agents was evaluated by disk diffusion method. E. coli isolates were sent to National Salmonella and Escherichia Centre, Central Research Institute, Kasauli, Himachal Pradesh for serotyping. Results: The result of present study revealed that out of 100 samples, 38 samples were found contaminated with E.coli. Antibiogram pattern revealed high resistance against ampicillin (100 %), whereas moderate resistance was observed for streptomycin (57.89 %), oxytetracycline (47.37 %) and amoxy-clav (42.11 %). Also lesser percentage of resistance was observed for co-trimoxazole (13.16 %) and chloramphenicol (5.26 %). Serotypes detected were O24 (7 isolates), followed by O36 (1 isolate), O89 (1 isolate), O91 (1 isolate) and O153(1 isolate). Conclusions: Results suggested a possibility of potential public health threat of E.coli originating from raw milk sources.

Prevalence and molecular characterization of extended-spectrum b-Lactamases (blaTEM) producing Escherichia coli isolated from humans and foods of animal origin in Chhattisgarh, India

“…However higher prevalence rates of 57% (Amin and Borah, 2002), 76% (Gangil et al, 2011) and 100% (Chaubey et al, 2004) were reported by different investigators. In present study, 20.19% milk samples were found positive for E. coli, however higher prevalence rates of 33.96% (Rashid et al, 2013), 38% (Thaker et al, 2012) and 43.3% (Rasheed et al, 2014) were reported from different regions. On the contrary lower prevalence rate of 15.5% (Gundogan and Avci, 2013) was recorded in raw milk samples in previous reports.…”

“…Imipenem is an antibiotic which is recently introduced and possibly resistance against it is not developed among E. coli isolates. Several other researchers have also reported similar resistance/sensitivity patterns of E. coli isolates against these antibiotics (Sharma et al, 2010, Thaker et al, 2012. Among the E. coli isolates, the highest value of MAR index reported was 1 for one isolate and the lowest MAR index reported was 0.16 for four isolates, two each from meat and milk.…”

Prevalence and Antimicrobial Susceptibility Pattern ofE. coliO157:H7 Isolated from Traditionally Marketed Raw Cow Milk in and around Asosa Town, Western Ethiopia

“…This prevalence is lower when compared to reports of [10] from Mekelle town, [16] from Britain, [17] from South India, and [18] from Malaysia who reported prevalence of E. coli from raw milk of 44.4%, 63%, 70%, and 65%, respectively. On the other hand, [19] reported a 38.0% prevalence from India…”

Occurrence and antibiotic resistance of Escherichia coli,Staphylococcus aureus and Bacillus cereus in raw milk and dairy products in Turkey

“…Escherichia coli is considered a faecal contamination indicator in foods because of its presence in the gut. The presence of E. coli in foods is a matter of concern because some strains may be pathogenic (Thaker et al 2012). Escherichia coli O157:H7 serotypes, identified as enterohaemorrhagic E. coli (EHEC) and grouped as verotoxin-producing E. coli (VTEC), are recognised as the primary cause of haemorrhagic colitis (HC) and the diarrhoea-associated form of haemolytic-uremic syndrome (HUS) (Rahimi et al 2011).…”

“…Consumable animal products have been suggested as a possible source of both resistant bacteria and resistant genes that can be transferred to humans directly (Pereira et al 2009). The antibiotic-resistant strains of a number of pathogenic bacteria, including S. aureus, B. cereus and E. coli, in foods which threaten public health have been the subject of many publications (Ozcelik and Citak 2009;Pereira et al 2009;Thaker et al 2012).…”