Study on membrane characteristics of alginate–chitosan microcapsule with cell growth

Wu, Yu; Song, Huiyi; Zheng, Guoshuang; Liu, Xiudong; Zhang, Ying; Ma, Xiaojun

doi:10.1016/j.memsci.2011.04.053

Cited by 46 publications

(14 citation statements)

References 35 publications

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“…Apparently, the emulsification/external gelation microcapsule has weak anti-swelling ability while being liquefied due to the irregular and non-uniform shape. However, the droplet-shape beads produced by emulsification/internal gelation results in clear and symmetrical membrane with strong anti-swelling ability (Yu et al, 2009;Yu et al, 2011).…”

Section: Characterization Of Ac Microcapsules Entrapping Yeast Cells mentioning

confidence: 99%

Microencapsulated probiotics using emulsification technique coupled with internal or external gelation process

Song

Gao

et al. 2013

Carbohydrate Polymers

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113

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Section: Characterization Of Ac Microcapsules Entrapping Yeast Cells mentioning

confidence: 99%

Microencapsulated probiotics using emulsification technique coupled with internal or external gelation process

Song

Gao

et al. 2013

Carbohydrate Polymers

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113

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“…Chitosan is a naturally positively charged polymer which provides the nanoparticles or microcapsules with the propensity to easily interact with negatively charged sites [21,22]. Under simultaneous electrostatic interaction between protonated amino groups of chitosan and carboxyl groups of alginate, polyelectrolyte complex microcapsules membrane are formed between chitosan and sodium alginate [23][24][25][26].…”

Section: Introductionmentioning

confidence: 99%

Microencapsulation of Traditional Chinese Herbs—PentaHerbs extracts and potential application in healthcare textiles

Chiu

Wang

Kan

et al. 2013

Colloids and Surfaces B: Biointerfaces

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“…3), thinner membrane may be too weak for a long‐time cell culture. Based on the results of our laboratory, the decline of substance permeability could happen during cell culture, just as the phenomenon occurred among the microcapsules with 10 μm membrane thickness 26, 28…”

Section: Resultsmentioning

confidence: 99%

“…The substance permeability of the microcapsule membrane with different thicknesses was also investigated during cell‐culture process. The culture medium is too complicated to analyze, and hence BSA was used as the model protein to evaluate the inward substance diffusion to the microcapsule 28. At the time of 0, 7, 14, and 21 days, APA microcapsules were collected, rinsed, and immersed into BSA solution.…”

Section: Resultsmentioning

confidence: 99%

Study of the effect of membrane thickness on microcapsule strength, permeability, and cell proliferation

Zhang

Wang

et al. 2012

J Biomedical Materials Res

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Cell microencapsulation is one of the promising strategies for in vitro production of proteins or in vivo delivery of therapeutic products. Membrane thickness controls microcapsule strength and permeability, which may in return affect cell growth and metabolism. In this study, the strength, permeability, and encapsulated Chinese hamster ovary cell proliferation and metabolism of four groups of microcapsules with different membrane thicknesses were investigated. It was found that increasing membrane thickness increases microcapsule strength, whereas decreases membrane permeability. During the first 6 days, cells within microcapsules with 10 lm thickness membrane proliferated fast and could reach a cell density of 1.9 Â 10 7 cells/mL microcapsule with 92% cell density. A cell density of 5.5 Â 10 7 cells/mL microcapsule with >85% cell density was achieved within microcapsules with 15 lm membrane thickness and these microcapsules kept over 88% integrity ratio after 11 days, which was much higher than that of microcapsules with 10 lm membrane thickness. Membrane with more than 20 lm thickness was not suited for encapsulated cell culture owing to low-protein diffusion rate. These results indicated that cells survived shortly within the thinnest membrane thickness. There was a specific membrane thickness more suitable for cell growth for a long-time culture. These findings will be useful for preparing microcapsules with the desired membrane thickness for microencapsulated cell culture dependent on various purposes.

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Study on membrane characteristics of alginate–chitosan microcapsule with cell growth

Cited by 46 publications

References 35 publications

Microencapsulated probiotics using emulsification technique coupled with internal or external gelation process

Microencapsulated probiotics using emulsification technique coupled with internal or external gelation process

Microencapsulation of Traditional Chinese Herbs—PentaHerbs extracts and potential application in healthcare textiles

Study of the effect of membrane thickness on microcapsule strength, permeability, and cell proliferation

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