Study on capillary electrophoresis–amperometric detection profiles of different parts of Morus alba L.

Chu, Qingcui; Lin, Miao; Tian, Xin; Ye, Jiannong

doi:10.1016/j.chroma.2006.03.118

Cited by 54 publications

(40 citation statements)

References 15 publications

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“…34,35 A ±30 kV high-voltage DC power supply (Shanghai Institute of Nuclear Research, China) provided a voltage between the ends of the capillary. The inlet of the capillary was held at a positive potential and the outlet end was maintained at ground.…”

Section: Apparatusmentioning

confidence: 99%

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Simultaneous Determination of Phenolic Additives in Cosmetics by Micellar Electrokinetic Capillary Chromatography with Electrochemical Detection

et al. 2010

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A micellar electrokinetic capillary chromatography method with electrochemical detection (MECC-ED) has been developed for the simultaneous determination of eight phenolic additives, including propyl gallate (PG), tert-butylhydroquinone (TBHQ), butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT), methylparaben (MP), ethylparaben (EP), propylparaben (PP) and butylparaben (BP) in cosmetic products. Method development involved optimization of the working electrode, the pH value of running buffer, the concentration of sodium dodecyl sulfate (SDS), the separation voltage, and the sample injection time. Under the optimum conditions, all analytes can be well separated within 26 min at the separation voltage of 18 kV in a 9 mmol•L -1 sodium dodecyl sulfate (SDS) -60 mmol•L -1 borate running buffer (pH 8.0). A 300 µm diameter carbon disk electrode generated good response at ＋0.90 V (vs. SCE) for all analytes. Linearity of the present method was over three orders of magnitude of analyte concentration with detection limits (S/N＝3) ranging from 1.1×10 -7 to 1.2×10 -6 g•mL -1 for allanalytes. This proposed method has been successfully applied to the simultaneous determination of the above additives in commercial cosmetics, and the assay results were satisfactory.

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Section: Apparatusmentioning

confidence: 99%

“…In addition, with electrochemical detection (ED), CE-ED affords high sensitivity and good selectivity for electroactive species. 34,35 However, to the best of our knowledge, MECC-ED has not been sufficiently employed for the purpose.…”

Section: Introductionmentioning

confidence: 97%

Simultaneous Determination of Phenolic Additives in Cosmetics by Micellar Electrokinetic Capillary Chromatography with Electrochemical Detection

et al. 2010

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“…In diabetic and hypercholesterolemic subjects, the supplementation of mulberry leaves can significantly reduce the levels of blood glucose, total cholesterol (TC), total triglycerides (TG), low-density lipoprotein (LDL) cholesterol, and very low-density lipoprotein cholesterol; this supplementation can also significantly increase the levels of high-density lipoprotein (HDL) cholesterol (Andallu et al, 2003). Furthermore, other bioactive ingredients of mulberry leaves have been identified including polyphenolic compounds, such as rutin, quercetin, chlorogenic acid, apigenin, and luteolin (Chu et al, 2006;Zhishen et al1999). This finding indicated possible antioxidant property of mulberry leaves (Arabshahi-Delouee and Urooj, 2007).…”

Section: Introductionmentioning

confidence: 99%

Addition of Mulberry Leaf (Morus Alba L.) to a Diet Formula Impeded Its Hypoglycemic Effect and Exacerbated Dyslipidemia in High-Fructose- And High-Fat-Induced Cd-1 Mice

et al. 2017

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Backgrounds: Morus alba L. (mulberry leaf) is frequently used for therapeutic purposes in China. This study investigated the hypoglycemic and hypolipidemic effects of a reported antidiabetic herbal formula (PLCP) supplemented with mulberry leaves (MPLCP) in high-fructose- and high-fat- (HFF) fed mice. Methods: Six-week-old CD-1 male mice were fed on normal standard diet for 10 weeks. Fat emulsion with or without aqueous/ethanol extracts of PLCP/MPLCP was administrated by gavage daily for HFF groups. The normal group only received vehicle. 15% Fructose drinking water was supplied for HFF groups. Results: MPLCP was less effective than PLCP in hypoglycemic effect. The addition of mulberry leaves impeded not only the decrease in insulin resistance and serum free fatty acid content but also the increase in spleen index. The ethanol extract of MPLCP caused remarkable increase in low-density lipoprotein levels (p < 0.01) and significant increase in total cholesterol level (p < 0.05) compared with model group. Thus the effect of MPLCP was detrimental to abnormal serum lipid levels. Conclusion: The addition of mulberry leaves to PLCP weakened the hypoglycemic effects of the original formula and exacerbated dyslipidemia.

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“…4 Though the fruits are delicious and edible, the anti-oxidant levels are found to be much higher in the leaves. 5 The major constituents of plant leaves include Chalcomoracin, Morachalcone A-C and flavanoids such as Luteolin, Rutin and Chlorogenic acid. [6][7][8] Hence, the present study is aimed at screening of extracts prepared using solvents with increasing polarity from theMorus alba leaves for their potential to inhibit free radicals (anti-oxidant activity) as well as to protect the DNA from free radical induced breaks (anti-clastogenic activity).…”

Section: Introductionmentioning

confidence: 99%

Comparative Assessment of the Anti-oxidant and Anti-clastogenic Activity of Morus alba Leaves

Raj

Mruthunjaya

Madhunapantula

et al. 2016

FRA

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Objective: The present study was conducted to investigate the antioxidant and anti-clastogenic activity of fractions extracted from Morusalba (Moraceae) leaves. Methods: The leaves of Morus alba were sequentially extracted using n-Hexane, Petroleum Ether, Ethyl Acetate, Methanol and Water, and Total phenolic content was determined using Folin-Ciocalteau (FC) reagent. The anti-oxidant property of each fraction was determined using 2-Deoxy-D-ribose, 2, 2-Diphenyl-1-picryl-hydrazyl (DPPH) and Hydroxyl radical scavenging assay. Furthermore, anti-clastogenic potential of these fractions was determined using calf-thymus DNA in the presence of Fenton's reagent followed by analyzing the samples by agarose gel electrophoresis. Results: All fractions showed the presence of varied amounts of total phenols and exhibited anti-oxidant activity as determined by scavenging the corresponding radicals. Among these fractions the methanol fraction (MEMA) showed the maximum phenol content as well as anti-oxidant activity. In addition MEMA could inhibit the breakage of DNA strands (anti-clastogenic activity) compared to other fractions tested. Discussion: Results of this study suggests that MEMA is likely to possess better antioxidant and anti-clastogenic activity.

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Study on capillary electrophoresis–amperometric detection profiles of different parts of Morus alba L.

Cited by 54 publications

References 15 publications

Simultaneous Determination of Phenolic Additives in Cosmetics by Micellar Electrokinetic Capillary Chromatography with Electrochemical Detection

Simultaneous Determination of Phenolic Additives in Cosmetics by Micellar Electrokinetic Capillary Chromatography with Electrochemical Detection

Addition of Mulberry Leaf (Morus Alba L.) to a Diet Formula Impeded Its Hypoglycemic Effect and Exacerbated Dyslipidemia in High-Fructose- And High-Fat-Induced Cd-1 Mice

Comparative Assessment of the Anti-oxidant and Anti-clastogenic Activity of Morus alba Leaves

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