Comparative Assessment of the Anti-oxidant and Anti-clastogenic Activity of Morus alba Leaves

Abstract

Objective: The present study was conducted to investigate the antioxidant and anti-clastogenic activity of fractions extracted from Morusalba (Moraceae) leaves. Methods: The leaves of Morus alba were sequentially extracted using n-Hexane, Petroleum Ether, Ethyl Acetate, Methanol and Water, and Total phenolic content was determined using Folin-Ciocalteau (FC) reagent. The anti-oxidant property of each fraction was determined using 2-Deoxy-D-ribose, 2, 2-Diphenyl-1-picryl-hydrazyl (DPPH) and Hydroxyl radical scavenging assay. Furthermore, anti-clastogenic potential of these fractions was determined using calf-thymus DNA in the presence of Fenton's reagent followed by analyzing the samples by agarose gel electrophoresis. Results: All fractions showed the presence of varied amounts of total phenols and exhibited anti-oxidant activity as determined by scavenging the corresponding radicals. Among these fractions the methanol fraction (MEMA) showed the maximum phenol content as well as anti-oxidant activity. In addition MEMA could inhibit the breakage of DNA strands (anti-clastogenic activity) compared to other fractions tested. Discussion: Results of this study suggests that MEMA is likely to possess better antioxidant and anti-clastogenic activity.

Neuroprotective and Cognitive Enhancing Effect of Methanolic Morus Alba Leaf Fraction in U87mg Cell Lines and Experimental Rat Model

“…Our inhouse data suggest that the methanol fraction of MA (MEMA) leaves could be used as a protective agent owing to its hydroxyl radical scavenging and anticlastogenic activity [14]. Furthermore, our unpublished in-house data, the leaf extracts have shown protective effects in amyloid-beta and scopolamine-induced animal models by elevating the endogenous antioxidant enzyme levels providing a base to the present work [15]. The present work aims to identify the potent neuroprotective effect of MA leaf fraction by subjecting them to in vitro and in vivo antioxidant tests.…”

“…Spatial learning and memory of the experimental animals were tested by the Barnes maze [15,19]. The apparatus consisted of a clear circular platform (13 cm×29 cm×14 cm) with 12 equally spaced holes (10 cm diameter) located 2 cm from the border.…”

Antitumor Activity of Potent Fraction of Lawsonia Inermis Root Extract by in Vitro and in Vivo Models

“…The free radical scavenging activity of all the fractions of Lawsonia inermis was assessed by calibrating the decrease in absorbance of their methanolic solutions. A solution of 0.1 mM DPPH was prepared in methanol and 2.4 ml of this solution was mixed with 1.6 ml of all the fractions of Lawsonia inermis at different concentrations (20, 40, 60, 80 and 100 µg/ml) the reaction mixtures were incubated in dark for 30 minutes and their absorbance were recorded at 517 nm wavelength against methanol as blank 12 . Ascorbic acid was used as the standard in the concentration range of 2-10 µg/ml.…”